Abstract
Carrot ( Daucus carota L.) In Vitro Regeneration Experiments were carried out for optimization of conditions for morphogenic callus production and regeneration of carrot ( Daucus carota L.) using four cultivars cultivated in Iran. Hypocotyl segments were put on media supplemented with 0.2 mg·L -1 2,4-D for callus initiation and proliferation. Small portions of callus (25 mg) were cut and transferred to new media with different concentrations of 2,4-D including 0.2, 0.5 and 1 mg·L -1 . MS (Murashige & Skoog 1962) and MSm (Masuda et al. 1981) media were used in the experiments to identify the differences between these two media according to callus, embryoid and plantlet production. Obtained results showed that low level of 2,4-D (0.2 mg·L -1 ) was more effective in morphogenic callus production although higher amounts of it (0.5 mg·L -1 and 1 mg·L -1 ) caused more callus growth. Kinetin in concentration of 0.1 mg·L -1 was more effective for regeneration than BAP used in concentration 1 mg·L -1 especially according to embryoid production. MSm medium was more useful than MS for callus production and regeneration of carrot plantlets. Among tested cultivars Nantes Improved had more capability to produce viable plantlets.
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Claims 5
Claim · model-written summary
Low 2,4-D at 0.2 mg·L -1 was more effective for morphogenic callus.
- Taxon
- carrot
Daucus carotat0 - Treatment
- 2,4-D (0.2 mg·L -1 )e0
- Outcome
- morphogenic callus productione0
- Direction
- more effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“low level of 2,4-D (0.2 mg·L -1 ) was more effective in morphogenic callus production”
t0“Carrot”
Claim · model-written summary
Higher 2,4-D at 0.5 and 1 mg·L -1 caused more callus growth.
- Taxon
- carrotDaucus carotat0
- Treatment
- 0.5 mg·L -1 and 1 mg·L -1e0
- Outcome
- callus growthe0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher amounts of it (0.5 mg·L -1 and 1 mg·L -1 ) caused more callus growth.”
t0“Carrot”
Claim · model-written summary
Kinetin at 0.1 mg·L -1 was better than BAP at 1 mg·L -1 for regeneration, especially embryoids.
- Taxon
- carrotDaucus carotat0
- Organ / stage
- embryoide0
- Treatment
- Kinetin in concentration of 0.1 mg·L -1e0
- Outcome
- regeneratione0
- Direction
- more effectivee0
- Comparator
- BAP used in concentration 1 mg·L -1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Kinetin in concentration of 0.1 mg·L -1 was more effective for regeneration than BAP used in concentration 1 mg·L -1 especially according to embryoid production.”
t0“Carrot”
Claim · model-written summary
MSm medium was more useful than MS for carrot callus production and plantlet regeneration.
- Taxon
- carrotDaucus carotae0
- Treatment
- MSm mediume0
- Outcome
- callus production and regeneration of carrot plantletse0
- Direction
- more usefule0
- Comparator
- MSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MSm medium was more useful than MS for callus production and regeneration of carrot plantlets.”
Claim · model-written summary
Nantes Improved produced more viable plantlets among tested cultivars.
- Taxon
- carrotDaucus carotat0
- Material
- Nantes Improvede0
- Outcome
- capability to produce viable plantletse0
- Direction
- moree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among tested cultivars Nantes Improved had more capability to produce viable plantlets.”
t0“Carrot”
The same questions in other papers
kinetin · growth · Daucus carota (carrot)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
in vitro · yield · Daucus carota (carrot)Positive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- no effect
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.