Paper · 1965 · Plant and Cell Physiology

CHANGES IN RESPONSE TO PURINE AND PYRIMIDINE DERIVATIVES OF CARROT ROOT CALLUS DURING SUCCESSIVE CULTURING

KUNIHIKO SYŌNO

15
claims
32
verbatim quotes
0
citations
1
research areas
—
Not open access

Abstract

The growth of the carrot root callus which had been subcultured for a long period (CCL) was promoted by the addition of 5×l0−8 and 5×l0−7 M kinetin, whereas in the callus subcultured for a short period (CCS) no growth promotion was observed at any concentrations of kinetin tested. CCL showed an increased growth in response to the application of kinetin, guanine, adenine, hypoxanthine, uracil, thymine, and cytosine in the presence of fractions A and C of carrot root extract, whereas no such response was observed in CCS. CCL required fraction C to respond to uracil and probably purine and pyrimidine derivatives in general. The growth of CCL was promoted by kinetin, guanine, adenine, or hypoxanthine in the medium containing inositol and amino acids mixture. In this case the growth-promoting action of guanine, adenine, or hypoxanthine was nullified by kinetin.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 15

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Kinetin at 5×l0−8 and 5×l0−7 M promoted growth of long-subcultured carrot root callus (CCL).

Taxon
carrotDaucus carotat0
Material
subcultured for a long period (CCL)e1
Organ / stage
root calluse1
Treatment
addition of 5×l0−8 and 5×l0−7 M kinetine1
Outcome
growthe1
Direction
promotede1
Comparator
callus subcultured for a short period (CCS)e2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“CARROT ROOT CALLUS”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
e1

“The growth of the carrot root callus which had been subcultured for a long period (CCL) was promoted by the addition of 5×l0−8 and 5×l0−7 M kinetin”

✓ located verbatim in the source · abstract, characters 0–147 · show in text ↑
e2

“callus subcultured for a short period (CCS)”

✓ located verbatim in the source · abstract, characters 164–207 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Short-subcultured carrot root callus (CCS) showed no growth promotion by any tested kinetin concentration.

Taxon
carrotDaucus carotat0
Material
callus subcultured for a short period (CCS)e2
Treatment
kinetine2
Outcome
growthe2
Direction
no growth promotione2
Comparator
subcultured for a long period (CCL)e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“CARROT ROOT CALLUS”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
e1

“The growth of the carrot root callus which had been subcultured for a long period (CCL) was promoted by the addition of 5×l0−8 and 5×l0−7 M kinetin”

✓ located verbatim in the source · abstract, characters 0–147 · show in text ↑
e2

“whereas in the callus subcultured for a short period (CCS) no growth promotion was observed at any concentrations of kinetin tested”

✓ located verbatim in the source · abstract, characters 149–280 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Guanine in the presence of fractions A and C increased growth of CCL, but not of CCS.

Taxon
carrotDaucus carotat0
Material
CCLe3
Treatment
guaninee3
Outcome
growthe3
Direction
increasede3
Qualifier
in the presence of fractions A and C of carrot root extracte3
Comparator
CCSe3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“CARROT ROOT CALLUS”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
e3

“CCL showed an increased growth in response to the application of kinetin, guanine, adenine, hypoxanthine, uracil, thymine, and cytosine in the presence of fractions A and C of carrot root extract, whereas no such response was observed in CCS”

✓ located verbatim in the source · abstract, characters 282–523 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Adenine in the presence of fractions A and C increased growth of CCL, but not of CCS.

Taxon
carrotDaucus carotat0
Material
CCLe3
Treatment
adeninee3
Outcome
growthe3
Direction
increasede3
Qualifier
in the presence of fractions A and C of carrot root extracte3
Comparator
CCSe3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“CARROT ROOT CALLUS”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
e3

“CCL showed an increased growth in response to the application of kinetin, guanine, adenine, hypoxanthine, uracil, thymine, and cytosine in the presence of fractions A and C of carrot root extract, whereas no such response was observed in CCS”

✓ located verbatim in the source · abstract, characters 282–523 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Hypoxanthine in the presence of fractions A and C increased growth of CCL, but not of CCS.

Taxon
carrotDaucus carotat0
Material
CCLe3
Treatment
hypoxanthinee3
Outcome
growthe3
Direction
increasede3
Qualifier
in the presence of fractions A and C of carrot root extracte3
Comparator
CCSe3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“CARROT ROOT CALLUS”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
e3

“CCL showed an increased growth in response to the application of kinetin, guanine, adenine, hypoxanthine, uracil, thymine, and cytosine in the presence of fractions A and C of carrot root extract, whereas no such response was observed in CCS”

✓ located verbatim in the source · abstract, characters 282–523 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Uracil in the presence of fractions A and C increased growth of CCL, but not of CCS.

Taxon
carrotDaucus carotat0
Material
CCLe3
Treatment
uracile3
Outcome
growthe3
Direction
increasede3
Qualifier
in the presence of fractions A and C of carrot root extracte3
Comparator
CCSe3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“CARROT ROOT CALLUS”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
e3

“CCL showed an increased growth in response to the application of kinetin, guanine, adenine, hypoxanthine, uracil, thymine, and cytosine in the presence of fractions A and C of carrot root extract, whereas no such response was observed in CCS”

✓ located verbatim in the source · abstract, characters 282–523 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Thymine in the presence of fractions A and C increased growth of CCL, but not of CCS.

Taxon
carrotDaucus carotat0
Material
CCLe3
Treatment
thyminee3
Outcome
growthe3
Direction
increasede3
Qualifier
in the presence of fractions A and C of carrot root extracte3
Comparator
CCSe3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“CARROT ROOT CALLUS”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
e3

“CCL showed an increased growth in response to the application of kinetin, guanine, adenine, hypoxanthine, uracil, thymine, and cytosine in the presence of fractions A and C of carrot root extract, whereas no such response was observed in CCS”

✓ located verbatim in the source · abstract, characters 282–523 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cytosine in the presence of fractions A and C increased growth of CCL, but not of CCS.

Taxon
carrotDaucus carotat0
Material
CCLe3
Treatment
cytosinee3
Outcome
growthe3
Direction
increasede3
Qualifier
in the presence of fractions A and C of carrot root extracte3
Comparator
CCSe3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“CARROT ROOT CALLUS”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
e3

“CCL showed an increased growth in response to the application of kinetin, guanine, adenine, hypoxanthine, uracil, thymine, and cytosine in the presence of fractions A and C of carrot root extract, whereas no such response was observed in CCS”

✓ located verbatim in the source · abstract, characters 282–523 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Kinetin promoted growth of CCL in a medium containing inositol and an amino acid mixture.

Taxon
carrotDaucus carotat0
Material
CCLe5
Treatment
kinetine5
Outcome
growthe5
Direction
promotede5
Qualifier
in the medium containing inositol and amino acids mixturee5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“CARROT ROOT CALLUS”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
e5

“The growth of CCL was promoted by kinetin, guanine, adenine, or hypoxanthine in the medium containing inositol and amino acids mixture”

✓ located verbatim in the source · abstract, characters 629–763 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Guanine promoted growth of CCL in a medium containing inositol and an amino acid mixture.

Taxon
carrotDaucus carotat0
Material
CCLe5
Treatment
guaninee5
Outcome
growthe5
Direction
promotede5
Qualifier
in the medium containing inositol and amino acids mixturee5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“CARROT ROOT CALLUS”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
e5

“The growth of CCL was promoted by kinetin, guanine, adenine, or hypoxanthine in the medium containing inositol and amino acids mixture”

✓ located verbatim in the source · abstract, characters 629–763 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Adenine promoted growth of CCL in a medium containing inositol and an amino acid mixture.

Taxon
carrotDaucus carotat0
Material
CCLe5
Treatment
adeninee5
Outcome
growthe5
Direction
promotede5
Qualifier
in the medium containing inositol and amino acids mixturee5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“CARROT ROOT CALLUS”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
e5

“The growth of CCL was promoted by kinetin, guanine, adenine, or hypoxanthine in the medium containing inositol and amino acids mixture”

✓ located verbatim in the source · abstract, characters 629–763 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Hypoxanthine promoted growth of CCL in a medium containing inositol and an amino acid mixture.

Taxon
carrotDaucus carotat0
Material
CCLe5
Treatment
hypoxanthinee5
Outcome
growthe5
Direction
promotede5
Qualifier
in the medium containing inositol and amino acids mixturee5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“CARROT ROOT CALLUS”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
e5

“The growth of CCL was promoted by kinetin, guanine, adenine, or hypoxanthine in the medium containing inositol and amino acids mixture”

✓ located verbatim in the source · abstract, characters 629–763 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Kinetin nullified the growth-promoting action of guanine, adenine or hypoxanthine.

Taxon
carrotDaucus carotat0
Treatment
kinetine6
Outcome
growth-promoting action of guanine, adenine, or hypoxanthinee6
Direction
nullifiede6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“CARROT ROOT CALLUS”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
e6

“In this case the growth-promoting action of guanine, adenine, or hypoxanthine was nullified by kinetin”

✓ located verbatim in the source · abstract, characters 765–867 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

CCL required fraction C to respond to uracil.

Taxon
carrotDaucus carotat0
Outcome
respond to uracile4
Qualifier
fraction Ce4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“CARROT ROOT CALLUS”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
e4

“CCL required fraction C to respond to uracil and probably purine and pyrimidine derivatives in general”

✓ located verbatim in the source · abstract, characters 525–627 · show in text ↑
Observationhypothesisoriginal resultmeasured

Claim · model-written summary

CCL probably also responds to purine and pyrimidine derivatives in general, requiring fraction C.

Taxon
carrotDaucus carotat0
Outcome
probably purine and pyrimidine derivatives in generale4
Qualifier
fraction Ce4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Daucus carota
Daucus carota · TaxonZeynel Cebeci · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“CARROT ROOT CALLUS”

✓ located verbatim in the source · title, characters 60–78 · show in text ↑
e4

“CCL required fraction C to respond to uracil and probably purine and pyrimidine derivatives in general”

✓ located verbatim in the source · abstract, characters 525–627 · show in text ↑

kinetin · growth · Daucus carota (carrot)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.