Abstract
Garlic cultivars are sexually sterile under standard growth conditions, with direct implications for commercial production costs as well as breeding programs. Garlic is propagated commercially via bulblets, which facilitates disease transmission and virus load accumulation over vegetative generations. Tissue culture produces virus-free clones that are more productive, while keeping the desired traits of the cultivar. Consequently, this technique allows studies of garlic genetics as well as guarantees genetic conservation of varieties. We aimed at analyzing the in vitro regeneration of eight marketable cultivars of garlic using root segments as explants. For each genotype, bulblet-derived explants were isolated and introduced into MS medium supplemented with 2,4-D and 2-iP. Calli were transferred to MS medium supplemented with 8.8 mM BAP and 0.1 mM NAA (regeneration medium A), or with 4.6 mM kinetin alone (regeneration medium B). The calli were then evaluated for regeneration frequency after sixty days of in vitro cultivation. The noble cultivar 'Jonas' presented the highest rates of plant regeneration among the cultivars tested. The medium A, which contained auxin and cytokinin, induced the highest regeneration rates of all cultivars. The process described herein is simple, reproducible and can potentially be used as a tool in molecular breeding strategies for other marketable cultivars and genotypes of garlic.
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Claims 3
Claim · model-written summary
Garlic cultivar 'Jonas' had the highest in vitro plant regeneration rate among the cultivars tested.
- Taxon
- garlic
Allium sativume3 - Material
- Jonase1
- Outcome
- rates of plant regeneratione1
- Direction
- higheste1
- Comparator
- the cultivars testede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The noble cultivar 'Jonas' presented the highest rates of plant regeneration among the cultivars tested”
e3“analyzing the in vitro regeneration of eight marketable cultivars of garlic”
Claim · model-written summary
Regeneration medium A, containing auxin and cytokinin, gave the highest regeneration rates across garlic cultivars.
- Taxon
- garlicAllium sativume3
- Treatment
- medium A, which contained auxin and cytokinine2
- Outcome
- regeneration ratese2
- Direction
- higheste2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“The medium A, which contained auxin and cytokinin, induced the highest regeneration rates of all cultivars”
e3“analyzing the in vitro regeneration of eight marketable cultivars of garlic”
Claim · model-written summary
The in vitro regeneration process is simple, reproducible and usable as a tool in garlic molecular breeding.
- Taxon
- garlicAllium sativume4
- Outcome
- tool in molecular breeding strategiese4
- Qualifier
- simple, reproduciblee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“The process described herein is simple, reproducible and can potentially be used as a tool in molecular breeding strategies for other marketable cultivars and genotypes of garlic”
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See all 26 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.