Abstract
Abstract: Tissue culture is considered as an alternative tool for garlic breeding as traditional breeding is not possible due to its sterility. A study was carried out to develop a protocol of regeneration in Bangladesh local garlic. Shoot and root meristems of garlic cv. Bangladesh local were cultured on Murashige and Skoog (MS) medium containing various growth regulator combinations. Regeneration from shoot meristem was found with all medium composition but highest (95.55%) in growth regulator-free medium. Shoots were transferred onto media containing BA or high level of sucrose. Forty per cent (40.00%) of the root meristem regenerated shoots on MS medium supplemented with 1 FM NAA and 10 FM BA. The shoots were multiplied on MS medium containing 0.5 FM BA. Bulblets were obtained from the regenerated shoots of garlic in all the media. It is the first to report regeneration from any Bangladeshi garlic. Key words: Allium sativum L., bulblets, meristem culture, regeneration, root tip culture Introduction variation through callus culture and production of new Garlic (Allium sativum L.) is an important and widely clones through somatic hybridization are the possible cultivated crop used for food and medicinal purposes. It steps that can be considered for the varietal improvement represents a significant crop in many regions of of garlic in Bangladesh. Production of virus-free clones Bangladesh. It is cultivated in 13,000 ha of land mostly through meristem tip culture has been reported elsewhere
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Claims 5
Claim · model-written summary
Shoot meristems regenerated in every tested medium composition.
- Taxon
- garlic
Allium sativume1 - Material
- Bangladesh locale1
- Organ / stage
- shoot meristeme0
- Outcome
- Regeneratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regeneration from shoot meristem was found with all medium composition”
e1“garlic cv. Bangladesh local”
Claim · model-written summary
Growth regulator-free medium yielded the highest shoot meristem regeneration, 95.55%.
- Taxon
- garlicAllium sativume1
- Treatment
- growth regulator-free mediume0
- Outcome
- Regeneration from shoot meristeme0
- Direction
- higheste0
- Value
- 95.55%e0
- Comparator
- all medium compositione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Regeneration from shoot meristem was found with all medium composition but highest (95.55%) in growth regulator-free medium”
e1“garlic cv. Bangladesh local”
Claim · model-written summary
Root meristems regenerated shoots at 40.00% on MS medium with NAA and BA.
- Taxon
- garlicAllium sativume1
- Treatment
- MS medium supplemented with 1 FM NAA and 10 FM BAe0
- Outcome
- root meristem regenerated shootse0
- Value
- 40.00%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Forty per cent (40.00%) of the root meristem regenerated shoots on MS medium supplemented with 1 FM NAA and 10 FM BA”
e1“garlic cv. Bangladesh local”
Claim · model-written summary
Shoots multiplied on MS medium containing 0.5 FM BA.
- Taxon
- garlicAllium sativume1
- Treatment
- MS medium containing 0.5 FM BAe0
- Outcome
- shoots were multipliede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The shoots were multiplied on MS medium containing 0.5 FM BA”
e1“garlic cv. Bangladesh local”
Claim · model-written summary
Regenerated garlic shoots produced bulblets in all media.
- Taxon
- garlicAllium sativume0
- Organ / stage
- regenerated shootse0
- Outcome
- Bulblets were obtainede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bulblets were obtained from the regenerated shoots of garlic in all the media”
The same questions in other papers
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See all 26 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.