Abstract
Flooding is a serious problem for soybeans because it reduces growth and grain yield. Proteomic and metabolomic techniques were used to examine whether mitochondrial function is altered in soybeans by flooding stress. Mitochondrial fractions were purified from the roots and hypocotyls of 4-day-old soybean seedlings that had been flooded for 2 days. Mitochondrial matrix and membrane proteins were separated by two-dimensional polyacrylamide gel electrophoresis and blue-native polyacrylamide gel electrophoresis, respectively. Differentially expressed proteins and metabolites were identified using mass spectrometry. Proteins and metabolites related to the tricarboxylic acid cycle and γ-amino butyrate shunt were up-regulated by flooding stress, while inner membrane carrier proteins and proteins related to complexes III, IV, and V of the electron transport chains were down-regulated. The amounts of NADH and NAD were increased; however, ATP was significantly decreased by flooding stress. These results suggest that flooding directly impairs electron transport chains, although NADH production increases in the mitochondria through the tricarboxylic acid cycle.
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Claims 5
Claim · model-written summary
Flooding up-regulated proteins and metabolites related to the tricarboxylic acid cycle and γ-amino butyrate shunt.
- Taxon
- Soybean
Glycine maxt0 - Treatment
- flooding stresse0
- Outcome
- Proteins and metabolites related to the tricarboxylic acid cycle and γ-amino butyrate shunte0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“Proteins and metabolites related to the tricarboxylic acid cycle and γ-amino butyrate shunt were up-regulated by flooding stress”
Claim · model-written summary
Flooding down-regulated inner membrane carrier and electron transport complex III, IV and V proteins.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- flooding stresse1
- Outcome
- inner membrane carrier proteins and proteins related to complexes III, IV, and V of the electron transport chainse0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“inner membrane carrier proteins and proteins related to complexes III, IV, and V of the electron transport chains were down-regulated”
e1“were up-regulated by flooding stress, while inner membrane carrier proteins”
Claim · model-written summary
Flooding increased mitochondrial NADH and NAD amounts.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- flooding stresse0
- Outcome
- amounts of NADH and NADe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“The amounts of NADH and NAD were increased; however, ATP was significantly decreased by flooding stress”
Claim · model-written summary
Flooding significantly decreased ATP.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- flooding stresse0
- Outcome
- ATPe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“ATP was significantly decreased by flooding stress”
Claim · model-written summary
The authors infer that flooding directly impairs mitochondrial electron transport despite increased NADH production through the tricarboxylic acid cycle.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- floodinge0
- Outcome
- electron transport chainse0
- Qualifier
- NADH production increases in the mitochondria through the tricarboxylic acid cyclee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“flooding directly impairs electron transport chains, although NADH production increases in the mitochondria through the tricarboxylic acid cycle”
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See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.