Abstract
Soybean is a widely cultivated crop; however, it is sensitive to flooding and drought stresses. The adverse environmental cues cause the endoplasmic reticulum (ER) stress due to accumulation of unfolded or misfolded proteins. To investigate the mechanisms in response to flooding and drought stresses, ER proteomics was performed in soybean root tips. The enzyme activity of NADH cytochrome c reductase was two-fold higher in the ER than other fractions, indicating that the ER was isolated with high purity. Protein abundance of ribosomal proteins was decreased under both stresses compared to control condition; however, the percentage of increased ribosomes was two-fold higher in flooding compared to drought. The ER proteins related to protein glycosylation and signaling were in response to both stresses. Compared to control condition, calnexin was decreased under both stresses; however, protein disulfide isomerase-like proteins and heat shock proteins were markedly decreased under flooding and drought conditions, respectively. Furthermore, fewer glycoproteins and higher levels of cytosolic calcium were identified under both stresses compared to control condition. These results suggest that reduced accumulation of glycoproteins in response to both stresses might be due to dysfunction of protein folding through calnexin/calreticulin cycle. Additionally, the increased cytosolic calcium levels induced by flooding and drought stresses might disturb the ER environment for proper protein folding in soybean root tips.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
ER NADH cytochrome c reductase activity was twice that in other fractions, indicating high ER purity.
- Taxon
- Soybean
Glycine maxe0t0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enzyme activity of NADH cytochrome c reductase was two-fold higher in the ER than other fractions, indicating that the ER was isolated with high purity.”
t0“Soybean”
Claim · model-written summary
Ribosomal protein abundance declined under flooding and drought relative to control.
- Taxon
- soybeanGlycine maxe0e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Protein abundance of ribosomal proteins was decreased under both stresses compared to control condition”
e1“response to flooding and drought stresses, ER proteomics was performed in soybean root tips.”
Claim · model-written summary
The proportion of increased ribosomes was twofold greater under flooding than drought.
- Taxon
- soybeanGlycine maxe0e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the percentage of increased ribosomes was two-fold higher in flooding compared to drought”
e1“ER proteomics was performed in soybean root tips”
Claim · model-written summary
ER proteins related to glycosylation and signaling responded to both stresses.
- Taxon
- soybeanGlycine maxe0e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ER proteins related to protein glycosylation and signaling were in response to both stresses.”
e1“ER proteomics was performed in soybean root tips”
Claim · model-written summary
Calnexin decreased under both stresses against control.
- Taxon
- soybeanGlycine maxe0e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to control condition, calnexin was decreased under both stresses”
e1“flooding and drought stresses, ER proteomics was performed in soybean root tips”
Claim · model-written summary
Protein disulfide isomerase-like proteins markedly decreased under flooding.
- Taxon
- soybeanGlycine maxe0e1e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“protein disulfide isomerase-like proteins and heat shock proteins were markedly decreased under flooding and drought conditions, respectively”
e1“Compared to control condition, calnexin was decreased under both stresses”
e2“ER proteomics was performed in soybean root tips”
Claim · model-written summary
Heat shock proteins markedly decreased under drought.
- Taxon
- soybeanGlycine maxe0e1e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“protein disulfide isomerase-like proteins and heat shock proteins were markedly decreased under flooding and drought conditions, respectively”
e1“Compared to control condition, calnexin was decreased under both stresses”
e2“ER proteomics was performed in soybean root tips”
Claim · model-written summary
Both stresses reduced glycoproteins relative to control.
- Taxon
- soybeanGlycine maxe0e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fewer glycoproteins and higher levels of cytosolic calcium were identified under both stresses compared to control condition”
e1“flooding and drought stresses, ER proteomics was performed in soybean root tips”
Claim · model-written summary
Both stresses increased cytosolic calcium relative to control.
- Taxon
- soybeanGlycine maxe0e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fewer glycoproteins and higher levels of cytosolic calcium were identified under both stresses compared to control condition”
e1“flooding and drought stresses, ER proteomics was performed in soybean root tips”
Claim · model-written summary
The authors propose that calnexin/calreticulin folding dysfunction explains reduced glycoprotein accumulation.
- Taxon
- soybeanGlycine maxe0e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduced accumulation of glycoproteins in response to both stresses might be due to dysfunction of protein folding through calnexin/calreticulin cycle”
e1“ER proteomics was performed in soybean root tips”
Claim · model-written summary
The authors propose that increased cytosolic calcium may disturb ER protein folding.
- Taxon
- soybeanGlycine maxe0
- Compound
- cytosolic calciume0
- Outcome
- proper protein foldinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the increased cytosolic calcium levels induced by flooding and drought stresses might disturb the ER environment for proper protein folding in soybean root tips”
The same questions in other papers
flooding · quality: protein · Glycine max (soybean)Results differ
4 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →flooding · molecular: expression · Glycine max (soybean)Positive and negative results
5 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- negative
3 papers more in this group
See all 7 papers →flooding · molecular: metabolites · Glycine max (soybean)Positive and negative results
11 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- negative
12 papers more in this group
See all 16 papers →drought · molecular: expression · Glycine max (soybean)Positive and negative results
21 papers in the same direction as this paper · 35 in the opposite direction
- positive
- positive
- positive
53 papers more in this group
See all 57 papers →drought · molecular: metabolites · Glycine max (soybean)Positive and negative results
39 papers in the same direction as this paper · 20 in the opposite direction
- positive
- positive
- positive
56 papers more in this group
See all 60 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.