Abstract
The Sinorhizobium fredii HH103 rkp-1 region, which is involved in capsular polysaccharides (KPS) production, was isolated and sequenced. The organization of the S. fredii genes identified, rkpUAGHIJ and kpsF3, was identical to that described for S. meliloti 1021 but different from that of S. meliloti AK631. The long rkpA gene (7.5 kb) of S. fredii HH103 and S. meliloti 1021 appears as a fusion of six clustered AK631 genes, rkpABCDEF. S. fredii HH103-Rif(r) mutants affected in rkpH or rkpG were constructed. An exoA mutant unable to produce exopolysaccharide (EPS) and a double mutant exoA rkpH also were obtained. Glycine max (soybean) and Cajanus cajan (pigeon pea) plants inoculated with the rkpH, rkpG, and rkpH exoA derivatives of S. fredii HH103 showed reduced nodulation and severe symptoms of nitrogen starvation. The symbiotic capacity of the exoA mutant was not significantly altered. All these results indicate that KPS, but not EPS, is of crucial importance for the symbiotic capacity of S. fredii HH103-Rif(r). S. meliloti strains that produce only EPS or KPS are still effective with alfalfa. In S. fredii HH103, however, EPS and KPS are not equivalent, because mutants in rkp genes are symbiotically impaired regardless of whether or not EPS is produced.
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Claims 8
Claim · model-written summary
The S. fredii rkp-1 region was isolated and sequenced.
- Taxon
- Soybean
Glycine maxt0 - Interactor
- Sinorhizobium fredii HH103Sinorhizobium fredii HH103e0symbiont
- Outcome
- rkp-1 region, which is involved in capsular polysaccharides (KPS) production, was isolated and sequencede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean and Cajanus cajan”
e0“The Sinorhizobium fredii HH103 rkp-1 region, which is involved in capsular polysaccharides (KPS) production, was isolated and sequenced.”
Claim · model-written summary
The identified S. fredii genes had the same organization as S. meliloti 1021 and a different organization from AK631.
- Taxon
- SoybeanGlycine maxt0
- Gene
- rkpUAGHIJ and kpsF3e0
- Outcome
- identical to that described for S. meliloti 1021 but different from that of S. meliloti AK631e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean and Cajanus cajan”
e0“The organization of the S. fredii genes identified, rkpUAGHIJ and kpsF3, was identical to that described for S. meliloti 1021 but different from that of S. meliloti AK631.”
Claim · model-written summary
The S. fredii rkpA gene appears to fuse six AK631 genes.
- Taxon
- SoybeanGlycine maxt0
- Gene
- rkpAe0
- Outcome
- appears as a fusion of six clustered AK631 genes, rkpABCDEFe0
- Value
- 7.5 kbe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean and Cajanus cajan”
e0“The long rkpA gene (7.5 kb) of S. fredii HH103 and S. meliloti 1021 appears as a fusion of six clustered AK631 genes, rkpABCDEF.”
Claim · model-written summary
Soybean inoculated with the rkpH, rkpG and rkpH exoA derivatives had reduced nodulation and severe nitrogen starvation.
- Taxon
- Glycine max (soybean)Glycine maxe0
- Treatment
- inoculated with the rkpH, rkpG, and rkpH exoA derivatives of S. fredii HH103e0
- Interactor
- S. fredii HH103
.JPG?width=60)
Sinorhizobium frediie0symbiont - Outcome
- nodulation and severe symptoms of nitrogen starvatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.JPG?width=500)
Verbatim
e0“Glycine max (soybean) and Cajanus cajan (pigeon pea) plants inoculated with the rkpH, rkpG, and rkpH exoA derivatives of S. fredii HH103 showed reduced nodulation and severe symptoms of nitrogen starvation.”
Claim · model-written summary
Pigeon pea inoculated with the rkpH, rkpG and rkpH exoA derivatives had reduced nodulation and severe nitrogen starvation.
- Taxon
- Cajanus cajan (pigeon pea)
Cajanus cajane0 - Treatment
- inoculated with the rkpH, rkpG, and rkpH exoA derivatives of S. fredii HH103e0
- Interactor
- S. fredii HH103Sinorhizobium frediie0symbiont
- Outcome
- nodulation and severe symptoms of nitrogen starvatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glycine max (soybean) and Cajanus cajan (pigeon pea) plants inoculated with the rkpH, rkpG, and rkpH exoA derivatives of S. fredii HH103 showed reduced nodulation and severe symptoms of nitrogen starvation.”
Claim · model-written summary
The exoA mutant did not significantly alter symbiotic capacity.
- Taxon
- SoybeanGlycine maxt0
- Material
- exoA mutante0
- Outcome
- symbiotic capacitye0
- Direction
- not significantly alterede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean and Cajanus cajan”
e0“The symbiotic capacity of the exoA mutant was not significantly altered.”
Claim · model-written summary
The authors infer KPS is crucial for S. fredii symbiotic capacity whereas EPS is not.
- Taxon
- SoybeanGlycine maxt0
- Interactor
- S. fredii HH103-Rif(r)Sinorhizobium frediie0symbiont
- Outcome
- symbiotic capacity of S. fredii HH103-Rif(r)e0
- Qualifier
- KPS, but not EPS, is of crucial importancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean and Cajanus cajan”
e0“All these results indicate that KPS, but not EPS, is of crucial importance for the symbiotic capacity of S. fredii HH103-Rif(r).”
Claim · model-written summary
S. meliloti strains producing only EPS or KPS remained effective with alfalfa.
- Taxon
- alfalfa
Medicago sativae0 - Interactor
- S. meliloti
Sinorhizobium melilotie0symbiont - Outcome
- still effective with alfalfae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“S. meliloti strains that produce only EPS or KPS are still effective with alfalfa.”
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See all 127 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.