Abstract
Phytic acid (myo-inositol hexakisphosphate) is the storage form of phosphorus in plant seeds. Reduction of phytic acid levels in seeds leads to both an increase in the availability of phosphorus and a reduction in the environmental load. Myo-inositol-1-phosphate (Ins(3)P1) synthase (EC 5.5.1.4) catalyzes the first step in phytic acid biosynthesis. We attempted to reduce the phytic acid content of seeds through manipulation of the expression of the Ins(3)P1 synthase gene. A rice Ins(3)P1 synthase gene, RINO1, was transformed into rice plants in the antisense or sense orientation under the control of the CaMV 35S promoter or the RINO1 promoter. Some T1 seeds that transformed both with RINO1 promoter::antisense RINO1 gene and with 35S promoter::sense RINO1 gene increased the amount of inorganic phosphates, as compared to those of non-transgenic plants. This result suggested a molar-equivalent decrease in phytic acid. We also examined RINO1 promoter activity using a β-glucuronidase (GUS) reporter gene. Finally, we discuss the strategy of molecular breeding to reduce phytic acid levels in seeds.
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Claims 3
Claim · model-written summary
Seeds transformed with RINO1 promoter::antisense RINO1 had increased inorganic phosphate relative to non-transgenic plants.
- Taxon
- rice
Oryza sativat0 - Organ / stage
- T1 seedse0
- Treatment
- RINO1 promoter::antisense RINO1 genee0
- Gene
- RINO1e0
- Outcome
- amount of inorganic phosphatese0
- Direction
- increasede0
- Comparator
- non-transgenic plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Some T1 seeds that transformed both with RINO1 promoter::antisense RINO1 gene and with 35S promoter::sense RINO1 gene increased the amount of inorganic phosphates, as compared to those of non-transgenic plants”
Claim · model-written summary
Seeds transformed with 35S promoter::sense RINO1 had increased inorganic phosphate relative to non-transgenic plants.
- Taxon
- riceOryza sativat0
- Organ / stage
- T1 seedse0
- Treatment
- 35S promoter::sense RINO1 genee0
- Gene
- RINO1e0
- Outcome
- amount of inorganic phosphatese0
- Direction
- increasede0
- Comparator
- non-transgenic plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“Some T1 seeds that transformed both with RINO1 promoter::antisense RINO1 gene and with 35S promoter::sense RINO1 gene increased the amount of inorganic phosphates, as compared to those of non-transgenic plants”
Claim · model-written summary
The phosphate increase suggested a molar-equivalent decrease in phytic acid.
- Taxon
- riceOryza sativat0
- Outcome
- molar-equivalent decrease in phytic acide0
- Qualifier
- This result suggestede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice”
e0“This result suggested a molar-equivalent decrease in phytic acid”