Paper · 2014 · Russian Journal of Genetics

Use of the common winter wheat homozygous population for genetic analysis of beta-amylase and evaluation of its aggregation ability

V. P. Netsvetaev; O. V. Akinshina; L. S. Bondarenko

9
claims
20
verbatim quotes
3
citations
3
research areas
—
Not open access

Abstract

We investigated a self-pollinated homozygous population of common winter wheat, F(-> ∞) 24/04 x Odesskaya krasnokolosaya, for variants of beta-amylase and the aggregation ability of the protein complex of weevil via disulfide bonds. It was found that variation in the electrophoretic types of this enzyme was due to four isoenzymes. Two of them (a and b) are doubled and controlled by separate loci with independent inheritance. Isoenzyme c was due to three dominant factors, and four loci were responsible for d. Analysis of the number of -S-S-bonds of five genotypes, which were harvested in 2013 and differed in the types of beta-amylase, showed that some of them were significantly different from others in this indicator. In general, the samples were grouped by the type of this enzyme, forming the following continuous series with respect to aggregation ability: I ≥ B ≥ F ≥ D ≥ G or 59.13 ± 3.18 ≥ 56.65 ± 2.46 ≥ 52.54 ± 2.24 ≥ 50.16 ± 1.67 ≥ 48.63 ± 6.25 of cond. units. Significant differences were observed for this property between groups B > D and I > D. Therefore, genotypes having types I and B have a positive influence on the rheological properties of dough.

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Claims 9

Observationobservedoriginal resultmeasured

Claim · model-written summary

Four isoenzymes explained electrophoretic variation in wheat beta-amylase.

Taxon
common winter wheate1
Gene
beta-amylasee1
Outcome
variation in the electrophoretic types of this enzymee0
Value
four isoenzymese0

Verbatim

e0

“variation in the electrophoretic types of this enzyme was due to four isoenzymes”

✓ located verbatim in the source · abstract, characters 251–331 · show in text ↑
e1

“common winter wheat, F(-> ∞) 24/04 x Odesskaya krasnokolosaya, for variants of beta-amylase”

✓ located verbatim in the source · abstract, characters 59–150 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Isoenzymes a and b were doubled and inherited at separate independent loci.

Taxon
common winter wheate1
Gene
a and be0
Outcome
doubled and controlled by separate loci with independent inheritancee0

Verbatim

e0

“Two of them (a and b) are doubled and controlled by separate loci with independent inheritance.”

✓ located verbatim in the source · abstract, characters 333–428 · show in text ↑
e1

“common winter wheat”

✓ located verbatim in the source · abstract, characters 59–78 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Three dominant factors controlled isoenzyme c.

Taxon
common winter wheate1
Gene
Isoenzyme ce0
Outcome
Isoenzyme c was due to three dominant factorse0
Value
three dominant factorse0

Verbatim

e0

“Isoenzyme c was due to three dominant factors”

✓ located verbatim in the source · abstract, characters 429–474 · show in text ↑
e1

“common winter wheat”

✓ located verbatim in the source · abstract, characters 59–78 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Four loci controlled isoenzyme d.

Taxon
common winter wheate1
Gene
de0
Outcome
four loci were responsible for de0
Value
four locie0

Verbatim

e0

“four loci were responsible for d”

✓ located verbatim in the source · abstract, characters 480–512 · show in text ↑
e1

“common winter wheat”

✓ located verbatim in the source · abstract, characters 59–78 · show in text ↑
Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Some of five wheat genotypes differed significantly in disulfide bond number.

Taxon
common winter wheate1
Material
five genotypese2
Outcome
number of -S-S-bondse2

Verbatim

e0

“some of them were significantly different from others in this indicator”

✓ located verbatim in the source · abstract, characters 654–725 · show in text ↑
e1

“common winter wheat”

✓ located verbatim in the source · abstract, characters 59–78 · show in text ↑
e2

“number of -S-S-bonds of five genotypes”

✓ located verbatim in the source · abstract, characters 530–568 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Aggregation ability ranked I ≥ B ≥ F ≥ D ≥ G.

Taxon
common winter wheate1
Outcome
aggregation abilitye2
Direction
I ≥ B ≥ F ≥ D ≥ Ge0
Value
59.13 ± 3.18 ≥ 56.65 ± 2.46 ≥ 52.54 ± 2.24 ≥ 50.16 ± 1.67 ≥ 48.63 ± 6.25 of cond. unitse0

Verbatim

e0

“I ≥ B ≥ F ≥ D ≥ G or 59.13 ± 3.18 ≥ 56.65 ± 2.46 ≥ 52.54 ± 2.24 ≥ 50.16 ± 1.67 ≥ 48.63 ± 6.25 of cond. units”

✓ located verbatim in the source · abstract, characters 869–977 · show in text ↑
e1

“common winter wheat”

✓ located verbatim in the source · abstract, characters 59–78 · show in text ↑
e2

“aggregation ability:”

✓ located verbatim in the source · abstract, characters 848–868 · show in text ↑
Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Aggregation ability was significantly greater for group B than D.

Taxon
common winter wheate1
Material
groups Be0
Outcome
this propertye0
Direction
B > De0
Comparator
De0

Verbatim

e0

“Significant differences were observed for this property between groups B > D and I > D.”

✓ located verbatim in the source · abstract, characters 979–1066 · show in text ↑
e1

“common winter wheat”

✓ located verbatim in the source · abstract, characters 59–78 · show in text ↑
Observationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Aggregation ability was significantly greater for group I than D.

Taxon
common winter wheate1
Material
Ie0
Outcome
this propertye0
Direction
I > De0
Comparator
De0

Verbatim

e0

“Significant differences were observed for this property between groups B > D and I > D.”

✓ located verbatim in the source · abstract, characters 979–1066 · show in text ↑
e1

“common winter wheat”

✓ located verbatim in the source · abstract, characters 59–78 · show in text ↑
Mechanismauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Genotypes I and B were interpreted to improve dough rheology.

Taxon
common winter wheate1
Material
types I and Be0
Outcome
rheological properties of doughe0

Verbatim

e0

“genotypes having types I and B have a positive influence on the rheological properties of dough”

✓ located verbatim in the source · abstract, characters 1078–1173 · show in text ↑
e1

“common winter wheat”

✓ located verbatim in the source · abstract, characters 59–78 · show in text ↑