Paper · 2004 · Journal of the Japanese Society for Horticultural Science

Changes in Sugar Metabolism Enzyme Activities in Cultivars of Two Pumpkin Species (Cucurbita maxima and C. moschata) during Fruit Development

Akira Tateishi; Taikai Nakayama; Katsunori Isobe; et al.

8
claims
17
verbatim quotes
7
citations
1
research areas
✓
Open access

Abstract

Sugar accumulation and sugar metabolism enzyme activities were measured in two distinct pumpkin cultivars, 'Miyako' (C. maxima) and 'Hayato' (C. moschata) during fruit development. Sugar accumulation levels in the fruits were different between these cultivars. At the harvesting stage, more glucose and fructose were accumulated in 'Hayato' than in 'Miyako'. Sucrose content was low in young fruit when its catabolic enzyme activities were highest. The accumulation of sucrose with fruit development in both cultivars coincided with increasing sucrose phosphate synthase (SPS) activity and decreasing catabolic enzyme activities. The amount of sucrose content was indistinguishable in both cultivars, whereas, higher SPS activity was observed in 'Hayato', SPS contributes to sucrose accumulation in the late stage of pumpkin fruit development, but the amount of accumulated sucrose could not be explained solely on SPS activity.

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Claims 8

Observationobservedoriginal resultmeasured

Claim · model-written summary

At harvest, Hayato accumulated more glucose and fructose than Miyako.

Taxon
Cucurbita maxima and C. moschataCucurbita maximaCucurbita moschatat0
Material
'Hayato'e0
Organ / stage
harvesting stagee0
Outcome
glucose and fructosee0
Direction
moree0
Comparator
'Miyako'e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucurbita maxima
Cucurbita maxima · Taxonmikami-kun · CC BY 4.0 · iNaturalist ↗
Cucurbita moschata
Cucurbita moschata · Taxonfr:User:Jeantosti · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“At the harvesting stage, more glucose and fructose were accumulated in 'Hayato' than in 'Miyako'”

✓ located verbatim in the source · abstract, characters 261–357 · show in text ↑
t0

“Cucurbita maxima and C. moschata”

✓ located verbatim in the source · title, characters 83–115 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Sucrose content was low in young fruit when catabolic enzyme activity was highest.

Taxon
Cucurbita maxima and C. moschataCucurbita maximaCucurbita moschatat0
Organ / stage
young fruite0
Gene
catabolic enzymee0
Outcome
Sucrose contente0
Direction
lowe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucurbita maxima
Cucurbita maxima · Taxonmikami-kun · CC BY 4.0 · iNaturalist ↗
Cucurbita moschata
Cucurbita moschata · Taxonfr:User:Jeantosti · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Sucrose content was low in young fruit when its catabolic enzyme activities were highest”

✓ located verbatim in the source · abstract, characters 359–447 · show in text ↑
t0

“Cucurbita maxima and C. moschata”

✓ located verbatim in the source · title, characters 83–115 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Sucrose accumulation during fruit development coincided with increasing SPS activity in both cultivars.

Taxon
Cucurbita maxima and C. moschataCucurbita maximaCucurbita moschatat0
Gene
sucrose phosphate synthase (SPS)e0
Outcome
accumulation of sucrosee0
Direction
accumulatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucurbita maxima
Cucurbita maxima · Taxonmikami-kun · CC BY 4.0 · iNaturalist ↗
Cucurbita moschata
Cucurbita moschata · Taxonfr:User:Jeantosti · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The accumulation of sucrose with fruit development in both cultivars coincided with increasing sucrose phosphate synthase (SPS) activity”

✓ located verbatim in the source · abstract, characters 449–585 · show in text ↑
t0

“Cucurbita maxima and C. moschata”

✓ located verbatim in the source · title, characters 83–115 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Sucrose accumulation during fruit development coincided with decreasing catabolic enzyme activity.

Taxon
Cucurbita maxima and C. moschataCucurbita maximaCucurbita moschatat0
Organ / stage
fruit developmente0
Outcome
catabolic enzyme activitiese0
Direction
decreasinge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucurbita maxima
Cucurbita maxima · Taxonmikami-kun · CC BY 4.0 · iNaturalist ↗
Cucurbita moschata
Cucurbita moschata · Taxonfr:User:Jeantosti · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The accumulation of sucrose with fruit development in both cultivars coincided with increasing sucrose phosphate synthase (SPS) activity and decreasing catabolic enzyme activities”

✓ located verbatim in the source · abstract, characters 449–628 · show in text ↑
t0

“Cucurbita maxima and C. moschata”

✓ located verbatim in the source · title, characters 83–115 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

Both cultivars had indistinguishable sucrose contents.

Taxon
Cucurbita maxima and C. moschataCucurbita maximaCucurbita moschatat0
Outcome
sucrose contente0
Direction
indistinguishablee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucurbita maxima
Cucurbita maxima · Taxonmikami-kun · CC BY 4.0 · iNaturalist ↗
Cucurbita moschata
Cucurbita moschata · Taxonfr:User:Jeantosti · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The amount of sucrose content was indistinguishable in both cultivars”

✓ located verbatim in the source · abstract, characters 630–699 · show in text ↑
t0

“Cucurbita maxima and C. moschata”

✓ located verbatim in the source · title, characters 83–115 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Hayato had higher SPS activity than Miyako.

Taxon
Cucurbita maxima and C. moschataCucurbita maximaCucurbita moschatat0
Material
'Hayato'e0
Outcome
SPS activitye0
Direction
highere0
Comparator
'Miyako'e1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucurbita maxima
Cucurbita maxima · Taxonmikami-kun · CC BY 4.0 · iNaturalist ↗
Cucurbita moschata
Cucurbita moschata · Taxonfr:User:Jeantosti · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“higher SPS activity was observed in 'Hayato'”

✓ located verbatim in the source · abstract, characters 710–754 · show in text ↑
e1

“'Miyako' (C. maxima) and 'Hayato' (C. moschata)”

✓ located verbatim in the source · abstract, characters 107–154 · show in text ↑
t0

“Cucurbita maxima and C. moschata”

✓ located verbatim in the source · title, characters 83–115 · show in text ↑
Mechanismauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

SPS contributes to sucrose accumulation late in pumpkin fruit development.

Taxon
Cucurbita maxima and C. moschataCucurbita maximaCucurbita moschatat0
Organ / stage
late stage of pumpkin fruit developmente0
Gene
SPSe0
Outcome
sucrose accumulatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucurbita maxima
Cucurbita maxima · Taxonmikami-kun · CC BY 4.0 · iNaturalist ↗
Cucurbita moschata
Cucurbita moschata · Taxonfr:User:Jeantosti · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“SPS contributes to sucrose accumulation in the late stage of pumpkin fruit development”

✓ located verbatim in the source · abstract, characters 756–842 · show in text ↑
t0

“Cucurbita maxima and C. moschata”

✓ located verbatim in the source · title, characters 83–115 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

SPS activity alone could not explain accumulated sucrose amounts.

Taxon
Cucurbita maxima and C. moschataCucurbita maximaCucurbita moschatat0
Gene
SPSe0
Outcome
amount of accumulated sucrosee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucurbita maxima
Cucurbita maxima · Taxonmikami-kun · CC BY 4.0 · iNaturalist ↗
Cucurbita moschata
Cucurbita moschata · Taxonfr:User:Jeantosti · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the amount of accumulated sucrose could not be explained solely on SPS activity”

✓ located verbatim in the source · abstract, characters 848–927 · show in text ↑
t0

“Cucurbita maxima and C. moschata”

✓ located verbatim in the source · title, characters 83–115 · show in text ↑