Abstract
A gene (At4g20010) involved in regulating flowering time in Arabidopsis thaliana (L.) Heynh. was identified by promoter trap T-DNA tagging. Plants containing a T-DNA insert in the 3'-UTR of At4g20010 flowered later under both long- and short-day conditions compared with control plants. Histochemical assays of the mutant plants showed that the promoterless gus gene is expressed predominantly in the shoot apex, but it is also expressed in root tips, stem nodes and in the abscission zone of developing siliques. Measurement of endogenous gibberellin (GA) showed that bioactive GA4 levels in mutant plants were reduced compared with wild type (WT) plants. Like other known mutants defective in GA biosynthesis, the late-flowering phenotype observed in our T-DNA-tagged line could be largely repressed by application of exogenous GA3. The T-DNA-tagged gene At4g20010 encodes a previously uncharacterised protein belonging to the DUF731 family. Sequence analysis showed similarity to a single-stranded binding domain and to an RNA-binding protein of Chlamydomonas reinhardtii. Considering the above results (sequence similarity, mutant phenotype and level of endogenous GA), we propose that At4g20010 is an RNA-binding protein involved in regulating GA biosynthesis, possibly at the post-transcriptional level.
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Claims 8
Claim · model-written summary
At4g20010 was identified as a flowering-time gene in Arabidopsis.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Gene
- At4g20010e0
- Outcome
- regulating flowering timee0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A gene (At4g20010) involved in regulating flowering time in Arabidopsis thaliana (L.) Heynh. was identified by promoter trap T-DNA tagging.”
Claim · model-written summary
At4g20010 T-DNA insertion plants flowered later than controls under long and short days.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- T-DNA insert in the 3'-UTR of At4g20010e1
- Gene
- At4g20010e1
- Outcome
- flowerede1
- Direction
- latere1
- Qualifier
- under both long- and short-day conditionse1
- Comparator
- control plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A gene (At4g20010) involved in regulating flowering time in Arabidopsis thaliana (L.) Heynh. was identified by promoter trap T-DNA tagging.”
e1“Plants containing a T-DNA insert in the 3'-UTR of At4g20010 flowered later under both long- and short-day conditions compared with control plants.”
Claim · model-written summary
The gus reporter was expressed mainly in the shoot apex and also in root tips, stem nodes and developing silique abscission zones.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- guse2
- Outcome
- expressed predominantly in the shoot apex, but it is also expressed in root tips, stem nodes and in the abscission zone of developing siliquese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A gene (At4g20010) involved in regulating flowering time in Arabidopsis thaliana (L.) Heynh. was identified by promoter trap T-DNA tagging.”
e2“the promoterless gus gene is expressed predominantly in the shoot apex, but it is also expressed in root tips, stem nodes and in the abscission zone of developing siliques.”
Claim · model-written summary
Mutant plants had reduced bioactive GA4 levels relative to wild type.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- mutant plantse3
- Outcome
- bioactive GA4 levelse3
- Direction
- reducede3
- Comparator
- wild type (WT) plantse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A gene (At4g20010) involved in regulating flowering time in Arabidopsis thaliana (L.) Heynh. was identified by promoter trap T-DNA tagging.”
e3“bioactive GA4 levels in mutant plants were reduced compared with wild type (WT) plants.”
Claim · model-written summary
Exogenous GA3 largely suppressed late flowering in the At4g20010 T-DNA line.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- T-DNA-tagged linee4
- Treatment
- application of exogenous GA3e4
- Outcome
- late-flowering phenotypee4
- Direction
- repressede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A gene (At4g20010) involved in regulating flowering time in Arabidopsis thaliana (L.) Heynh. was identified by promoter trap T-DNA tagging.”
e4“the late-flowering phenotype observed in our T-DNA-tagged line could be largely repressed by application of exogenous GA3.”
Claim · model-written summary
At4g20010 encodes a previously uncharacterised DUF731 family protein.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- At4g20010e5
- Outcome
- previously uncharacterised protein belonging to the DUF731 familye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A gene (At4g20010) involved in regulating flowering time in Arabidopsis thaliana (L.) Heynh. was identified by promoter trap T-DNA tagging.”
e5“The T-DNA-tagged gene At4g20010 encodes a previously uncharacterised protein belonging to the DUF731 family.”
Claim · model-written summary
At4g20010 sequence resembles a single-stranded binding domain and an RNA-binding protein of Chlamydomonas reinhardtii.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- At4g20010e0
- Outcome
- similarity to a single-stranded binding domain and to an RNA-binding protein of Chlamydomonas reinhardtiie6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A gene (At4g20010) involved in regulating flowering time in Arabidopsis thaliana (L.) Heynh. was identified by promoter trap T-DNA tagging.”
e6“Sequence analysis showed similarity to a single-stranded binding domain and to an RNA-binding protein of Chlamydomonas reinhardtii.”
Claim · model-written summary
The authors propose that At4g20010 regulates GA biosynthesis as an RNA-binding protein, possibly post-transcriptionally.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- At4g20010e7
- Outcome
- regulating GA biosynthesise7
- Qualifier
- possibly at the post-transcriptional levele7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A gene (At4g20010) involved in regulating flowering time in Arabidopsis thaliana (L.) Heynh. was identified by promoter trap T-DNA tagging.”
e7“we propose that At4g20010 is an RNA-binding protein involved in regulating GA biosynthesis, possibly at the post-transcriptional level.”