Abstract
The phytohormone abscisic acid (ABA) induces genes-encoding proteins involved in desiccation tolerance and dormancy in seeds, but ABA also suppresses gibberellin (GA)-responsive genes encoding hydrolytic enzymes essential for postgermination growth. A unique serine/threonine protein kinase, PKABA1 mRNA, up-regulated by ABA in seeds, has been identified. In this report, the effect of PKABA1 on the signal transduction pathway mediating ABA induction and suppression of genes has been determined in aleurone layers of barley seeds. Two groups of gene constructs were introduced to barley aleurone layers by using particle bombardment: the reporter constructs containing the coding sequence of beta-glucuronidase gene linked to hormone-responsive promoters and the effector constructs containing the coding region of protein kinases linked to a constitutive promoter. Constitutive expression of PKABA1 drastically suppressed expression of low- and high-pI alpha-amylase and protease genes induced by GA. However, the presence of PKABA1 had only a small effect on the ABA induction of a gene encoding a late embryogenesis abundant protein, HVA1. Our results indicate that PKABA1 acts as a key intermediate in the signal transduction pathway leading to the suppression of GA-inducible gene expression in cereal aleurone layers.
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Claims 3
Claim · model-written summary
Constitutive PKABA1 expression strongly suppressed GA-induced alpha-amylase and protease genes.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaret0 - Treatment
- Constitutive expression of PKABA1e0
- Gene
- PKABA1e0
- Outcome
- expression of low- and high-pI alpha-amylase and protease genese0
- Direction
- drastically suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Constitutive expression of PKABA1 drastically suppressed expression of low- and high-pI alpha-amylase and protease genes induced by GA”
e1“Constitutive expression of PKABA1 drastically suppressed expression of low- and high-pI alpha-amylase and protease genes induced by GA”
t0“barley”
Claim · model-written summary
PKABA1 had a small effect on ABA induction of HVA1.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- presence of PKABA1e0
- Gene
- PKABA1e0
- Outcome
- ABA induction of a gene encoding a late embryogenesis abundant protein, HVA1e0
- Qualifier
- only a small effecte0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the presence of PKABA1 had only a small effect on the ABA induction of a gene encoding a late embryogenesis abundant protein, HVA1”
e1“the presence of PKABA1 had only a small effect on the ABA induction of a gene encoding a late embryogenesis abundant protein, HVA1”
t0“barley”
Claim · model-written summary
The authors interpret PKABA1 as an intermediate in suppression of GA-inducible expression.
- Taxon
- barleyHordeum vulgaret0
- Gene
- PKABA1e0
- Outcome
- suppression of GA-inducible gene expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PKABA1 acts as a key intermediate in the signal transduction pathway leading to the suppression of GA-inducible gene expression”
e1“PKABA1 acts as a key intermediate in the signal transduction pathway leading to the suppression of GA-inducible gene expression”
t0“barley”
The same questions in other papers
overexpression · molecular: expression · Hordeum vulgare (barley)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.