Abstract
Smooth microsomal membranes were isolated from axes of soybean (Glycine max L. Merr.) seeds at the dehydration-tolerant (6 hours of imbibition) and dehydration-susceptible (36 hours of imbibition) stages of development and were exposed to free radicals in vitro using xanthine-xanthine oxidase as a free radical source. Wide angle x-ray diffraction studies indicated that the lipid phase transition temperature of the microsomal membranes from the dehydration-tolerant axes increased from 7 to 14 degrees C after exposure to free radicals, whereas those from the dehydration-susceptible axes increased from 9 to 40 degrees C by the same free radical dose. The increased phase transition temperature was associated with a decrease in the phospholipid:sterol ratio, and an increase in the free fatty acid:phospholipid ratio. There was no significant change in total fatty acid saturation, which indicated that free radical treatment induced deesterification of membrane phospholipid, and not a change in fatty acid saturation. Similar compositional and structural changes have been previously observed in dehydration-injured soybean axes suggesting that dehydration may induce free radical injury to cellular membranes. Further, these membranes differ in their susceptibility to free radical injury, presumably reflecting compositional differences in the membrane since these membranes were exposed to free radicals in the absence of cytosol.
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Claims 7
Claim · model-written summary
Free radicals increased membrane lipid phase transition temperature from 7 to 14 degrees C in tolerant axes.
- Taxon
- soybean
Glycine maxt0 - Organ / stage
- dehydration-tolerant axese0
- Treatment
- exposure to free radicalse0
- Outcome
- lipid phase transition temperaturee0
- Direction
- increasede0
- Value
- 14 degrees Ce0
- Comparator
- 7 to 14 degrees Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the lipid phase transition temperature of the microsomal membranes from the dehydration-tolerant axes increased from 7 to 14 degrees C after exposure to free radicals”
t0“Soybean Axes”
t1“Soybean Axes”
Claim · model-written summary
Free radicals increased membrane lipid phase transition temperature from 9 to 40 degrees C in susceptible axes.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- dehydration-susceptible axese0
- Treatment
- free radical dosee0
- Outcome
- those from the dehydration-susceptible axese0
- Direction
- increasede0
- Value
- 40 degrees Ce0
- Comparator
- 9 to 40 degrees Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“those from the dehydration-susceptible axes increased from 9 to 40 degrees C by the same free radical dose.”
t0“Soybean Axes”
t1“Soybean Axes”
Claim · model-written summary
Higher transition temperature was associated with lower phospholipid-to-sterol ratio.
- Taxon
- soybeanGlycine maxt0
- Compound
- phospholipide0
- Outcome
- phospholipid:sterol ratioe0
- Direction
- decreasee0
- Qualifier
- increased phase transition temperaturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increased phase transition temperature was associated with a decrease in the phospholipid:sterol ratio, and an increase in the free fatty acid:phospholipid ratio.”
t0“Soybean Axes”
t1“Soybean Axes”
Claim · model-written summary
Higher transition temperature was associated with higher free-fatty-acid-to-phospholipid ratio.
- Taxon
- soybeanGlycine maxt0
- Compound
- phospholipide0
- Outcome
- free fatty acid:phospholipid ratioe0
- Direction
- increasee0
- Qualifier
- increased phase transition temperaturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increased phase transition temperature was associated with a decrease in the phospholipid:sterol ratio, and an increase in the free fatty acid:phospholipid ratio.”
t0“Soybean Axes”
t1“Soybean Axes”
Claim · model-written summary
Total fatty acid saturation did not change significantly under free radical treatment.
- Taxon
- soybeanGlycine maxt0
- Treatment
- free radical treatmente0
- Outcome
- total fatty acid saturatione0
- Direction
- no significant changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was no significant change in total fatty acid saturation, which indicated that free radical treatment induced deesterification of membrane phospholipid, and not a change in fatty acid saturation.”
t0“Soybean Axes”
t1“Soybean Axes”
Claim · model-written summary
Free radical exposure induced membrane phospholipid deesterification.
- Taxon
- soybeanGlycine maxt0
- Treatment
- free radical treatmente0
- Outcome
- deesterification of membrane phospholipide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“free radical treatment induced deesterification of membrane phospholipid”
t0“Soybean Axes”
t1“Soybean Axes”
Claim · model-written summary
Membranes from tolerant and susceptible axes differed in susceptibility to free radical injury.
- Taxon
- soybeanGlycine maxt0
- Outcome
- susceptibility to free radical injurye0
- Qualifier
- presumably reflecting compositional differences in the membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these membranes differ in their susceptibility to free radical injury, presumably reflecting compositional differences in the membrane since these membranes were exposed to free radicals in the absence of cytosol.”
t0“Soybean Axes”
t1“Soybean Axes”