Abstract
Protein ubiquitination is a posttranslational regulatory process essential for plant growth and interaction with the environment. E3 ligases, to which the seven in absentia (SINA) proteins belong, determine the specificity by selecting the target proteins for ubiquitination. SINA proteins are found in animals as well as in plants, and a small gene family with highly related members has been identified in the genome of rice (Oryza sativa), Arabidopsis (Arabidopsis thaliana), Medicago truncatula, and poplar (Populus trichocarpa). To acquire insight into the function of SINA proteins in nodulation, a dominant negative form of the Arabidopsis SINAT5 was ectopically expressed in the model legume M. truncatula. After rhizobial inoculation of the 35S:SINAT5DN transgenic plants, fewer nodules were formed than in control plants, and most nodules remained small and white, a sign of impaired symbiosis. Defects in rhizobial infection and symbiosome formation were observed by extensive microscopic analysis. Besides the nodulation phenotype, transgenic plants were affected in shoot growth, leaf size, and lateral root number. This work illustrates a function for SINA E3 ligases in a broad spectrum of plant developmental processes, including nodulation.
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Claims 5
Claim · model-written summary
Rhizobially inoculated SINAT5DN plants formed fewer nodules than controls.
- Taxon
- Medicago truncatula
Medicago truncatulat0 - Material
- 35S:SINAT5DN transgenic plantse0
- Treatment
- rhizobial inoculatione0
- Interactor
- rhizobiale0symbiont
- Outcome
- nodulese0
- Direction
- fewere0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After rhizobial inoculation of the 35S:SINAT5DN transgenic plants, fewer nodules were formed than in control plants”
t0“Medicago truncatula”
Claim · model-written summary
Most nodules remained small and white, indicating impaired symbiosis.
- Taxon
- Medicago truncatulaMedicago truncatulat0
- Outcome
- nodules remained small and whitee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most nodules remained small and white, a sign of impaired symbiosis.”
t0“Medicago truncatula”
Claim · model-written summary
Microscopy showed defects in rhizobial infection and symbiosome formation.
- Taxon
- Medicago truncatulaMedicago truncatulat0
- Interactor
- rhizobiale0symbiont
- Outcome
- Defects in rhizobial infection and symbiosome formatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Defects in rhizobial infection and symbiosome formation were observed by extensive microscopic analysis.”
t0“Medicago truncatula”
Claim · model-written summary
Transgenic plants had altered shoot growth, leaf size and lateral root number.
- Taxon
- Medicago truncatulaMedicago truncatulat0
- Material
- transgenic plantse0
- Outcome
- shoot growth, leaf size, and lateral root numbere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic plants were affected in shoot growth, leaf size, and lateral root number.”
t0“Medicago truncatula”
Claim · model-written summary
The authors infer SINA E3 ligases function in development and nodulation.
- Taxon
- Medicago truncatulaMedicago truncatulat0
- Gene
- SINA E3 ligasese0
- Outcome
- plant developmental processes, including nodulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This work illustrates a function for SINA E3 ligases in a broad spectrum of plant developmental processes, including nodulation.”
t0“Medicago truncatula”
The same questions in other papers
inoculation · symbiosis · Medicago truncatula (barrel medic)Positive and negative results
1 paper in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.