Abstract
Calli were initiated from 2,627 of approximately 65,000 anthers of oats ( Avena sativa L.) placed on various culture media. One haploid (n = 3x = 21) and two diploid (2n = 6x = 42) plants were recovered from an anther of the cultivar ‘Stout’. These were the first plants known to be producebdy anther culture in oats. Ther elated cultivars Stout and ‘Clintford’ were the source of most of the anthers which formed callus. These lines responded with more than 30% anthers callusing under defined culture conditions, while no other genotypes among over 100 initially tested had more than 6% anthers callusing and most had none. When Clintford was crossed with five nonresponding genotypes, the F 1 plants from three of the crosses produced relatively high frequency anther‐callusing responses while F 1 plants of the other two crosses gave low responses indicating a complex inheritance of the trait. The highest frequency of callusing anthers was achieved with a Stout ✕ Clintford F 2 plant where 12 of 15 cultured anthers responded. Murashige and Skoog basal medium with 10% sucrose and no hormonegs ave the highest anther callus initiation frequencies among media tested, but the only anther to produce plants had been initially plated on a modified potato extract medium containing 2 mg/ liter 2,4‐dichlorophenoxy acetic acid and 0.5 mg/liter kinetin. This anther had also been heat‐shocked at 35 C for 24 hours immediately after plating and prior to standard incubation at 22 C. Cold shocks reduced sucrose levels, and the addition of various growth substances had either no or detrimental effects on callus initiation frequencies and did not promote plant differentiation. Means to regenerate plants from initiated calli on a consistent basis are needed to make oat anther culture a useful technique.
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Claims 13
Claim · model-written summary
Calli were initiated from 2,627 of approximately 65,000 oat anthers.
- Taxon
- Oat
Avena sativat0 - Organ / stage
- antherse0
- Outcome
- Calli were initiatede0
- Value
- 2,627e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oat Anther Culture”
e0“Calli were initiated from 2,627 of approximately 65,000 anthers of oats ( Avena sativa L.)”
Claim · model-written summary
One haploid and two diploid plants were recovered from an anther of the cultivar Stout.
- Taxon
- OatAvena sativat0
- Material
- Stoute1
- Organ / stage
- an anthere1
- Outcome
- plants were recoverede1
- Value
- One haploid (n = 3x = 21) and two diploid (2n = 6x = 42) plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oat Anther Culture”
e1“One haploid (n = 3x = 21) and two diploid (2n = 6x = 42) plants were recovered from an anther of the cultivar ‘Stout’. These were the first plants known to be producebdy anther culture in oats”
Claim · model-written summary
These were the first plants known to be produced by anther culture in oats.
- Taxon
- oatsAvena sativae1
- Outcome
- These were the first plants knowne1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oat Anther Culture”
e1“One haploid (n = 3x = 21) and two diploid (2n = 6x = 42) plants were recovered from an anther of the cultivar ‘Stout’. These were the first plants known to be producebdy anther culture in oats”
Claim · model-written summary
Anthers of the cultivar Stout responded with more than 30% callusing under defined culture conditions.
- Taxon
- OatAvena sativat0
- Material
- Stoute2
- Outcome
- anthers callusinge2
- Value
- more than 30%e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oat Anther Culture”
e2“Ther elated cultivars Stout and ‘Clintford’ were the source of most of the anthers which formed callus. These lines responded with more than 30% anthers callusing under defined culture conditions”
Claim · model-written summary
No other of over 100 initially tested genotypes had more than 6% anther callusing.
- Taxon
- OatAvena sativat0
- Outcome
- anthers callusinge3
- Value
- more than 6%e3
- Qualifier
- no other genotypes among over 100 initially testede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oat Anther Culture”
e3“while no other genotypes among over 100 initially tested had more than 6% anthers callusing”
Claim · model-written summary
F1 plants from three of the crosses of Clintford with five nonresponding genotypes produced relatively high frequencies of anther-callusing responses.
- Taxon
- OatAvena sativat0
- Material
- Clintforde4
- Treatment
- crossed with five nonresponding genotypese4
- Outcome
- anther‐callusing responsese4
- Direction
- relatively highe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oat Anther Culture”
e4“When Clintford was crossed with five nonresponding genotypes, the F 1 plants from three of the crosses produced relatively high frequency anther‐callusing responses while F 1 plants of the other two crosses gave low responses indicating a complex inheritance of the trait”
Claim · model-written summary
F1 plants of the other two Clintford crosses gave low anther-callusing responses.
- Taxon
- OatAvena sativat0
- Material
- Clintforde4
- Treatment
- crossed with five nonresponding genotypese4
- Outcome
- anther‐callusing responsese4
- Direction
- low responsese4
- Comparator
- three of the crossese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oat Anther Culture”
e4“When Clintford was crossed with five nonresponding genotypes, the F 1 plants from three of the crosses produced relatively high frequency anther‐callusing responses while F 1 plants of the other two crosses gave low responses indicating a complex inheritance of the trait”
Claim · model-written summary
Murashige and Skoog basal medium with 10% sucrose gave the highest anther callus initiation frequencies among the media tested.
- Taxon
- OatAvena sativat0
- Treatment
- Murashige and Skoog basal medium with 10% sucrosee6
- Outcome
- anther callus initiation frequenciese6
- Direction
- higheste6
- Comparator
- media testede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oat Anther Culture”
e6“Murashige and Skoog basal medium with 10% sucrose and no hormonegs ave the highest anther callus initiation frequencies among media tested, but the only anther to produce plants had been initially plated on a modified potato extract medium containing 2 mg/ liter 2,4‐dichlorophenoxy acetic acid and 0.5 mg/liter kinetin”
Claim · model-written summary
The only anther that produced plants had been plated initially on a modified potato extract medium.
- Taxon
- OatAvena sativat0
- Organ / stage
- anthere6
- Treatment
- modified potato extract mediume6
- Outcome
- produce plantse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oat Anther Culture”
e6“Murashige and Skoog basal medium with 10% sucrose and no hormonegs ave the highest anther callus initiation frequencies among media tested, but the only anther to produce plants had been initially plated on a modified potato extract medium containing 2 mg/ liter 2,4‐dichlorophenoxy acetic acid and 0.5 mg/liter kinetin”
Claim · model-written summary
Cold shocks reduced sucrose levels.
- Taxon
- OatAvena sativat0
- Treatment
- Cold shockse7
- Outcome
- sucrose levelse7
- Direction
- reducede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oat Anther Culture”
e7“Cold shocks reduced sucrose levels, and the addition of various growth substances had either no or detrimental effects on callus initiation frequencies and did not promote plant differentiation. Means to regenerate plants from initiated calli on a consistent basis are needed to make oat anther culture a useful technique”
Claim · model-written summary
Growth substances had either no or detrimental effects on callus initiation frequencies.
- Taxon
- OatAvena sativat0
- Treatment
- growth substancese7
- Outcome
- callus initiation frequenciese7
- Direction
- detrimental effectse7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oat Anther Culture”
e7“Cold shocks reduced sucrose levels, and the addition of various growth substances had either no or detrimental effects on callus initiation frequencies and did not promote plant differentiation. Means to regenerate plants from initiated calli on a consistent basis are needed to make oat anther culture a useful technique”
Claim · model-written summary
Growth substances did not promote plant differentiation.
- Taxon
- OatAvena sativat0
- Treatment
- growth substancese7
- Outcome
- plant differentiatione7
- Direction
- did not promotee7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oat Anther Culture”
e7“Cold shocks reduced sucrose levels, and the addition of various growth substances had either no or detrimental effects on callus initiation frequencies and did not promote plant differentiation. Means to regenerate plants from initiated calli on a consistent basis are needed to make oat anther culture a useful technique”
Claim · model-written summary
Means to regenerate plants from initiated calli on a consistent basis are needed for oat anther culture to be useful.
- Taxon
- OatAvena sativat0
- Outcome
- Means to regenerate plants from initiated callie7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oat Anther Culture”
e7“Cold shocks reduced sucrose levels, and the addition of various growth substances had either no or detrimental effects on callus initiation frequencies and did not promote plant differentiation. Means to regenerate plants from initiated calli on a consistent basis are needed to make oat anther culture a useful technique”
The same questions in other papers
sucrose · growth · Avena sativa (oats)Positive and negative results
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- negative
- positive
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See all 5 papers →in vitro · growth · Avena sativa (oats)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction · 2 reporting no difference from an alternative
- negative
- no effect
- no effect
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.