Abstract
The effect of protein additives on acetylene reduction (N(2) fixation) by Rhizobium associated with soybean cells (Glycine max [L.] Merr.) in vitro was studied. Acetylene reduction was promoted on the basal medium supplemented with 1.4 mg of N/ml supplied as aqueous extracts of hexane-extracted soybean, red kidney beans (Phaseolus vulgaris L.), or peas (Pisum sativum L.). Commercial samples of alpha-casein, or bovine serum albumin also promoted acetylene reduction at a concentration of 1.4 mg of N/ml of basal medium, but egg albumin supplying an equal amount of nitrogen to the basal medium completely suppressed acetylene reduction. Autoclaving the aqueous extract of hexane-extracted soybean meal had no effect on its ability to promote acetylene reduction. The presence of 40 mm succinate decreased acetylene reduction with leguminous proteins supplying 1.4 mg of N/ml but promoted acetylene reduction by Rhizobium 32H1-soybean cell associations on media containing alpha-casein, bovine serum albumin, or egg albumin suppling 1.4 mg of N/ml. Similar results were obtained with both cowpea Rhizobium 32H1 and Rhizobium japonicum 61A96. Pure cultures of Rhizobium 32H1 developed acetylene-reducing activity in the presence of soybean extract on basal agar medium and in vermiculite supplied with N-free mineral salts plus crude soybean meal. The results suggest that in certain situations, free living Rhizobium may reduce N(2) under field conditions.
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Claims 10
Claim · model-written summary
Legume protein extracts promoted acetylene reduction in soybean-associated Rhizobium.
- Taxon
- soybean
Glycine maxe1 - Treatment
- aqueous extracts of hexane-extracted soybean, red kidney beans (Phaseolus vulgaris L.), or peas (Pisum sativum L.)e0
- Outcome
- Acetylene reductione0
- Direction
- promotede0
- Value
- 1.4 mg of N/mle0
RhizobiumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Acetylene reduction was promoted on the basal medium supplemented with 1.4 mg of N/ml supplied as aqueous extracts of hexane-extracted soybean, red kidney beans (Phaseolus vulgaris L.), or peas (Pisum sativum L.).”
e1“Rhizobium associated with soybean cells (Glycine max [L.] Merr.)”
Claim · model-written summary
Alpha-casein promoted acetylene reduction in soybean-associated Rhizobium.
- Taxon
- soybeanGlycine maxe1
- Treatment
- alpha-caseine0
- Outcome
- acetylene reductione0
- Direction
- promotede0
- Value
- 1.4 mg of N/mle0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Commercial samples of alpha-casein, or bovine serum albumin also promoted acetylene reduction at a concentration of 1.4 mg of N/ml of basal medium”
e1“Rhizobium associated with soybean cells (Glycine max [L.] Merr.)”
Claim · model-written summary
Bovine serum albumin promoted acetylene reduction in soybean-associated Rhizobium.
- Taxon
- soybeanGlycine maxe1
- Treatment
- bovine serum albumine0
- Outcome
- acetylene reductione0
- Direction
- promotede0
- Value
- 1.4 mg of N/mle0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Commercial samples of alpha-casein, or bovine serum albumin also promoted acetylene reduction at a concentration of 1.4 mg of N/ml of basal medium”
e1“Rhizobium associated with soybean cells (Glycine max [L.] Merr.)”
Claim · model-written summary
Egg albumin completely suppressed acetylene reduction.
- Taxon
- soybeanGlycine maxe1
- Treatment
- egg albumine0
- Outcome
- acetylene reductione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“egg albumin supplying an equal amount of nitrogen to the basal medium completely suppressed acetylene reduction”
e1“Rhizobium associated with soybean cells (Glycine max [L.] Merr.)”
Claim · model-written summary
Autoclaving soybean meal extract did not change its promotion of acetylene reduction.
- Taxon
- soybeanGlycine maxe1
- Treatment
- Autoclaving the aqueous extract of hexane-extracted soybean meale0
- Outcome
- ability to promote acetylene reductione0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Autoclaving the aqueous extract of hexane-extracted soybean meal had no effect on its ability to promote acetylene reduction.”
e1“Rhizobium associated with soybean cells (Glycine max [L.] Merr.)”
Claim · model-written summary
Succinate decreased acetylene reduction with leguminous proteins.
- Taxon
- soybeanGlycine maxe1
- Treatment
- 40 mm succinatee0
- Outcome
- acetylene reductione0
- Direction
- decreasede0
- Qualifier
- leguminous proteins supplying 1.4 mg of N/mle0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of 40 mm succinate decreased acetylene reduction with leguminous proteins supplying 1.4 mg of N/ml”
e1“Rhizobium associated with soybean cells (Glycine max [L.] Merr.)”
Claim · model-written summary
Succinate promoted acetylene reduction by Rhizobium 32H1 soybean cell associations with animal proteins.
- Taxon
- soybeanGlycine maxe0
- Treatment
- 40 mm succinatee1
- Interactor
- Rhizobium 32H1e0symbiont
- Outcome
- acetylene reduction by Rhizobium 32H1e0
- Direction
- promotede0
- Qualifier
- media containing alpha-casein, bovine serum albumin, or egg albumin suppling 1.4 mg of N/mle0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promoted acetylene reduction by Rhizobium 32H1-soybean cell associations on media containing alpha-casein, bovine serum albumin, or egg albumin suppling 1.4 mg of N/ml”
e1“The presence of 40 mm succinate”
Claim · model-written summary
The response was similar for cowpea Rhizobium 32H1 and Rhizobium japonicum 61A96.
- Taxon
- soybeanGlycine maxe1
- Interactor
- cowpea Rhizobium 32H1e0symbiont
- Second interactor
- Rhizobium japonicum 61A96e0symbiont
- Outcome
- Similar resultse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Similar results were obtained with both cowpea Rhizobium 32H1 and Rhizobium japonicum 61A96.”
e1“soybean cells (Glycine max [L.] Merr.)”
Claim · model-written summary
Pure Rhizobium 32H1 cultures developed acetylene-reducing activity with soybean extract on agar.
- Taxon
- soybeanGlycine maxe0
- Interactor
- Rhizobium 32H1e0symbiont
- Outcome
- Rhizobium 32H1 developed acetylene-reducing activitye0
- Setting
- basal agar mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pure cultures of Rhizobium 32H1 developed acetylene-reducing activity in the presence of soybean extract on basal agar medium”
Claim · model-written summary
Pure Rhizobium 32H1 cultures developed acetylene-reducing activity with crude soybean meal in vermiculite.
- Taxon
- soybeanGlycine maxe0
- Interactor
- Rhizobium 32H1e0symbiont
- Outcome
- Rhizobium 32H1 developed acetylene-reducing activitye0
- Setting
- vermiculitee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pure cultures of Rhizobium 32H1 developed acetylene-reducing activity in the presence of soybean extract on basal agar medium and in vermiculite supplied with N-free mineral salts plus crude soybean meal.”
The same questions in other papers
succinate · symbiosis · Glycine max (soybean)Results agree
1 paper in the same direction as this paper
- positive
botanical · Rhizobium · Glycine max (soybean)Positive and negative results
1 in the opposite direction
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.