Abstract
Cannabis sativa L. is an extraordinarily versatile plant that has been valued since Neolithic times. Since C. sativa is a cross pollinated crop, the allogamous (cross fertilization) nature of the species makes it impossible to maintain elite cultivar/clones by seed. Synthetic seed technology could be a valuable aid for economical large-scale clonal propagation and germplasm conservation of the screened and selected elite germplasm of C. sativa. Nodal segements containing axillary buds of Cannabis sativa isolated from multiple shoot cultures were encapsulated in 5% sodium alginate hydrogel. Regrowth and conversion after encapsulation was evaluated both under in vitro and in vivo conditions on different planting substrates. The addition of antimicrobial substance – Plant Preservative Mixture, PPM had a positive effect on overall plantlet development. Encapsulated explants exhibited the best regrowth and conversion frequency on Murashige and Skoog medium supplemented with TDZ (0.5 µM) and PPM (0.075%) under in vitro conditions and 100% conversion was obtained on 1:1 potting mix- fertilome with coco natural growth medium, moistened with full strength MS medium without plant growth regulators supplemented with 3% sucrose and 0.5% PPM under in vivo conditions. Plantlets regenerated from the encapsulated explants were hardened off and successfully transferred to soil. The advantages of the encapsulation procedure for clonal mass propagation of elite varieties of C. sativa over the presently employed shoot organogenesis are discussed in relation to economy of space, time and overall cost. Acknowledgement: The work was supported in part by National Institute of Drug Abuse (NIDA), Contract No. N01DA-0-7707 and United States Department of Agriculture Agricultural Research Service Specific Cooperative Agreement No. 58-6408-6-067.
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Claims 4
Claim · model-written summary
PPM improved overall plantlet development.
- Taxon
- Cannabis sativa
Cannabis sativae1 - Treatment
- Plant Preservative Mixture, PPMe0
- Outcome
- overall plantlet developmente0
- Direction
- positive effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of antimicrobial substance – Plant Preservative Mixture, PPM had a positive effect on overall plantlet development.”
e1“Nodal segements containing axillary buds of Cannabis sativa”
Claim · model-written summary
Encapsulated explants had their best in vitro regrowth and conversion on MS medium with TDZ and PPM.
- Taxon
- Cannabis sativaCannabis sativae1
- Treatment
- Murashige and Skoog medium supplemented with TDZ (0.5 µM) and PPM (0.075%)e0
- Outcome
- regrowth and conversion frequencye0
- Direction
- beste0
- Setting
- in vitro conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Encapsulated explants exhibited the best regrowth and conversion frequency on Murashige and Skoog medium supplemented with TDZ (0.5 µM) and PPM (0.075%) under in vitro conditions”
e1“Nodal segements containing axillary buds of Cannabis sativa”
Claim · model-written summary
In vivo conversion reached 100% on the described potting mixture and medium.
- Taxon
- Cannabis sativaCannabis sativae1
- Treatment
- 1:1 potting mix- fertilome with coco natural growth medium, moistened with full strength MS medium without plant growth regulators supplemented with 3% sucrose and 0.5% PPMe0
- Outcome
- conversione0
- Value
- 100%e0
- Setting
- in vivo conditionse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“100% conversion was obtained on 1:1 potting mix- fertilome with coco natural growth medium, moistened with full strength MS medium without plant growth regulators supplemented with 3% sucrose and 0.5% PPM under in vivo conditions.”
e1“Nodal segements containing axillary buds of Cannabis sativa”
Claim · model-written summary
Regenerated plantlets were hardened and transferred to soil.
- Taxon
- Cannabis sativaCannabis sativae1
- Outcome
- successfully transferred to soile0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plantlets regenerated from the encapsulated explants were hardened off and successfully transferred to soil.”
e1“Nodal segements containing axillary buds of Cannabis sativa”
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