Abstract
• In an effort to understand processes that are related to heavy metal sequestration, we characterized six metallothionein genes (PtdMTs) in the hybrid cottonwood, Populus trichocarpa × deltoides. • cDNA microarrays and reverse transcriptase-polymerase chain reaction were used to examine PtdMT expression in poplar tissues. They displayed differential gene expression patterns, which may be associated with the diverse roles and functions PtdMTs have in coping with particular developmental (e.g. root development and leaf senescence) and environmental cues. • The heterologous expression in a cadmium (Cd)-hypersensitive yeast mutant showed the ability of PtdMT cDNAs to confer Cd tolerance. The concentration of PtdMT mRNAs were increased by zinc, but not by copper and Cd. • Further studies will help to clarify the role of metallothionein genes in metal homeostasis and poplar development, and help to isolate poplar genotypes particularly tolerant to stress for use in experiments of phytoremediation.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Six metallothionein genes were characterized in hybrid cottonwood.
- Taxon
- Populus trichocarpa × deltoidesPopulus trichocarpa ×Populus deltoidese0
- Gene
- PtdMTse0
- Outcome
- six metallothionein genese0
- Value
- sixe0
Verbatim
e0“we characterized six metallothionein genes (PtdMTs) in the hybrid cottonwood, Populus trichocarpa × deltoides.”
Claim · model-written summary
PtdMTs showed differential expression among poplar tissues.
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtdMT expression in poplar tissues. They displayed differential gene expression patterns”
Claim · model-written summary
PtdMT cDNAs conferred cadmium tolerance in a hypersensitive yeast mutant.
- Treatment
- heterologous expressione0
- Gene
- PtdMT cDNAse0
- Outcome
- Cd tolerancee0
- Qualifier
- cadmium (Cd)-hypersensitive yeast mutante0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“hybrid poplar”
e0“The heterologous expression in a cadmium (Cd)-hypersensitive yeast mutant showed the ability of PtdMT cDNAs to confer Cd tolerance.”
Claim · model-written summary
Zinc increased PtdMT mRNA concentrations.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“hybrid poplar”
e0“The concentration of PtdMT mRNAs were increased by zinc, but not by copper and Cd.”
Claim · model-written summary
Copper did not increase PtdMT mRNA concentrations.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“hybrid poplar”
e0“The concentration of PtdMT mRNAs were increased by zinc, but not by copper and Cd.”
Claim · model-written summary
Cadmium did not increase PtdMT mRNA concentrations.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“hybrid poplar”
e0“The concentration of PtdMT mRNAs were increased by zinc, but not by copper and Cd.”
The same questions in other papers
copper atom · molecular: metabolites · Populus (poplar)Positive and negative results
2 in the opposite direction
- positive
- positive
cadmium atom · molecular: metabolites · Populus (poplar)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
zinc atom · molecular: metabolites · Populus (poplar)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.