Abstract
Cucumber mosaic virus (CMV), a member of the Cucumovirus genus, is the causal agent of several plant diseases in a wide range of host species, causing important economic losses in agriculture. Because of the lack of natural resistance genes in most crops, different genetic engineering strategies have been adopted to obtain virus-resistant plants. In a previous study, we described the engineering of transgenic tomato plants expressing a single-chain variable fragment antibody (scFv G4) that are specifically protected from CMV infection. In this work, we characterized the leaf proteome expressed during compatible plant-virus interaction in wild type and transgenic tomato. Protein changes in both inoculated and apical leaves were revealed using two-dimensional gel electrophoresis (2-DE) coupled to differential in gel electrophoresis (DIGE) technology. A total of 2084 spots were detected, and 50 differentially expressed proteins were identified by nanoscale liquid chromatographic-electrospray ionization-ion trap-tandem mass spectrometry (nLC-ESI-IT-MS/MS). The majority of these proteins were related to photosynthesis (38%), primary metabolism (18%), and defense activity (14%) and demonstrated to be actively down regulated by CMV in infected leaves. Moreover, our analysis revealed that asymptomatic apical leaves of transgenic inoculated plants had no protein profile alteration as compared to control wild type uninfected plants demonstrating that virus infection is confined to the inoculated leaves and systemic spread is hindered by the CMV coat protein (CP)-specific scFv G4 molecules. Our work is the first comparative study on compatible plant-virus interactions between engineered immunoprotected and susceptible wild type tomato plants, contributing to the understanding of antibody-mediated disease resistance mechanisms.
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Claims 8
Claim · model-written summary
Proteome analysis detected 2084 spots.
- Taxon
- tomato
Solanum lycopersicumt0 - Outcome
- spotse0
- Value
- 2084e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 2084 spots were detected”
t0“Proteomic Analysis of the Plant−Virus Interaction in Cucumber Mosaic Virus (CMV) Resistant Transgenic Tomato”
Claim · model-written summary
Fifty differentially expressed proteins were identified.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- differentially expressed proteinse0
- Value
- 50e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“50 differentially expressed proteins were identified”
t0“tomato”
Claim · model-written summary
Of identified proteins, 38% related to photosynthesis.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- proteins were related to photosynthesise0
- Value
- 38%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The majority of these proteins were related to photosynthesis (38%), primary metabolism (18%), and defense activity (14%)”
t0“tomato”
Claim · model-written summary
Of identified proteins, 18% related to primary metabolism.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- primary metabolisme0
- Value
- 18%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The majority of these proteins were related to photosynthesis (38%), primary metabolism (18%), and defense activity (14%)”
t0“tomato”
Claim · model-written summary
Of identified proteins, 14% related to defense activity.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- defense activitye0
- Value
- 14%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The majority of these proteins were related to photosynthesis (38%), primary metabolism (18%), and defense activity (14%)”
t0“tomato”
Claim · model-written summary
CMV downregulated the photosynthesis, primary metabolism, and defense proteins in infected leaves.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- CMVe0
- Interactor
- CMV
cucumber mosaic cucumoviruse0pathogen - Outcome
- these proteinse0
- Direction
- down regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these proteins were related to photosynthesis (38%), primary metabolism (18%), and defense activity (14%) and demonstrated to be actively down regulated by CMV in infected leaves”
t0“tomato”
Claim · model-written summary
Apical leaves of inoculated transgenic plants had no protein profile alteration versus uninfected wild type.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- apical leavese0
- Outcome
- protein profile alteratione0
- Direction
- no protein profile alteratione0
- Comparator
- control wild type uninfected plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“asymptomatic apical leaves of transgenic inoculated plants had no protein profile alteration as compared to control wild type uninfected plants”
t0“tomato”
Claim · model-written summary
The authors infer CMV infection stayed in inoculated leaves and systemic spread was hindered by scFv G4.
- Taxon
- tomatoSolanum lycopersicumt0
- Interactor
- CMVcucumber mosaic cucumoviruse0pathogen
- Gene
- scFv G4e0
- Outcome
- systemic spreade0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“virus infection is confined to the inoculated leaves and systemic spread is hindered by the CMV coat protein (CP)-specific scFv G4 molecules”
t0“tomato”
The same questions in other papers
Cucumis sativus · cucumber mosaic cucumovirus · Solanum lycopersicum (tomato)Positive and negative results
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