Abstract
Preharvest calcium application has been shown to increase broccoli microgreen yield and extend shelf life. In this study, we investigated the effect of calcium application on its metabolome using ultra-high-performance liquid chromatography with mass spectrometry. The data collected were analyzed using principal component analysis and orthogonal projection to latent structural discriminate analysis. Chemical composition comparison shows that glucosinolates, a very important group of phytochemicals, are the major compounds enhanced by preharvest treatment with 10 mM calcium chloride (CaCl2). Aliphatic glucosinolates (glucoerucin, glucoiberin, glucoiberverin, glucoraphanin, pentyl glucosinolate, and hexyl glucosinolate) and indolic glucosinolates (glucobrassicin, neoglucobrassicin, and 4-hydroxyglucobrassicin) were increased significantly in the CaCl2 treated microgreens using metabolomic approaches. Targeted glucosinolate analysis using the ISO 9167-1 method was further employed to confirm the findings. Results indicate that glucosinolates can be considered as a class of compounds that are responsible for the difference between two groups and a higher glucosinolate level was found in CaCl2 treated groups at each time point after harvest in comparison with the control group.
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Claims 5
Claim · model-written summary
Preharvest calcium chloride enhanced glucosinolates.
- Taxon
- Broccoli
Brassica oleracea var. italicat0 - Organ / stage
- Microgreenst0
- Treatment
- preharvest treatment with 10 mM calcium chloride (CaCl2)e0
- Outcome
- glucosinolatese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glucosinolates, a very important group of phytochemicals, are the major compounds enhanced by preharvest treatment with 10 mM calcium chloride (CaCl2)”
t0“Broccoli Microgreens”
Claim · model-written summary
Aliphatic glucosinolates increased significantly in treated microgreens.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- CaCl2e0
- Outcome
- Aliphatic glucosinolatese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aliphatic glucosinolates (glucoerucin, glucoiberin, glucoiberverin, glucoraphanin, pentyl glucosinolate, and hexyl glucosinolate) and indolic glucosinolates (glucobrassicin, neoglucobrassicin, and 4-hydroxyglucobrassicin) were increased significantly in the CaCl2 treated microgreens”
t0“Broccoli Microgreens”
Claim · model-written summary
Indolic glucosinolates increased significantly in treated microgreens.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- CaCl2e0
- Outcome
- indolic glucosinolatese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aliphatic glucosinolates (glucoerucin, glucoiberin, glucoiberverin, glucoraphanin, pentyl glucosinolate, and hexyl glucosinolate) and indolic glucosinolates (glucobrassicin, neoglucobrassicin, and 4-hydroxyglucobrassicin) were increased significantly in the CaCl2 treated microgreens”
t0“Broccoli Microgreens”
Claim · model-written summary
Targeted ISO analysis confirmed the glucosinolate findings.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Outcome
- confirm the findingse0
- Qualifier
- ISO 9167-1 methode0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Targeted glucosinolate analysis using the ISO 9167-1 method was further employed to confirm the findings”
t0“Broccoli Microgreens”
Claim · model-written summary
Treated microgreens had higher glucosinolate levels at every postharvest time point than controls.
- Taxon
- BroccoliBrassica oleracea var. italicat0
- Treatment
- CaCl2e0
- Outcome
- glucosinolate levele0
- Direction
- highere0
- Qualifier
- at each time point after harveste0
- Comparator
- control groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a higher glucosinolate level was found in CaCl2 treated groups at each time point after harvest in comparison with the control group”
t0“Broccoli Microgreens”
The same questions in other papers
calcium chloride dihydrate · quality: phytochemicals · Brassica oleracea (wild cabbage)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.