Abstract
Glucosinolates are important phytochemicals in Brassicaceae. We investigated the effect of CaCl2-HCl electrolyzed water (CHEW) on glucosinolates biosynthesis in broccoli sprouts. The results showed that CHEW treatment significantly decreased reactive oxygen species (ROS) and malondialdeh yde (MDA) contents in broccoli sprouts. On the the 8th day, compared to tap water treatment, the the total glucosinolate content of broccoli sprouts with CHEW treatment increased by 10.6% and calcium content was dramatically enhanced from 14.4 mg/g DW to 22.7 mg/g DW. Comparative transcriptome and metabolome analyses revealed that CHEW treatment activated ROS and calcium signaling transduction pathways in broccoli sprouts and they interacted through MAPK cascades. Besides, CHEW treatment not only promoted the biosynthesis of amino acids, but also enhanced the expression of structural genes in glucosinolate synthesis through transcription factors (MYBs, bHLHs, WRKYs, etc.). The results of this study provided new insights into the regulatory network of glucosinolates biosynthesis in broccoli sprouts under CHEW treatment.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
CHEW treatment significantly decreased ROS and MDA contents in broccoli sprouts.
- Taxon
- broccoli
Brassica oleracea var. italicae1 - Treatment
- CHEW treatmente1
- Outcome
- reactive oxygen species (ROS) and malondialdeh yde (MDA) contentse1
- Direction
- decreasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in broccoli sprouts”
e1“The results showed that CHEW treatment significantly decreased reactive oxygen species (ROS) and malondialdeh yde (MDA) contents in broccoli sprouts”
Claim · model-written summary
Total glucosinolate content of broccoli sprouts under CHEW treatment increased by 10.6% compared with tap water.
- Taxon
- broccoliBrassica oleracea var. italicae2
- Treatment
- CHEW treatmente2
- Outcome
- total glucosinolate contente2
- Direction
- increasede2
- Value
- 10.6%e2
- Comparator
- tap water treatmente2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in broccoli sprouts”
e2“compared to tap water treatment, the the total glucosinolate content of broccoli sprouts with CHEW treatment increased by 10.6%”
Claim · model-written summary
Calcium content in broccoli sprouts was dramatically enhanced under CHEW treatment, from 14.4 to 22.7 mg/g DW.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Treatment
- CHEW treatmente2
- Outcome
- calcium contente3
- Direction
- enhancede3
- Value
- from 14.4 mg/g DW to 22.7 mg/g DWe3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in broccoli sprouts”
e2“compared to tap water treatment, the the total glucosinolate content of broccoli sprouts with CHEW treatment increased by 10.6%”
e3“calcium content was dramatically enhanced from 14.4 mg/g DW to 22.7 mg/g DW”
Claim · model-written summary
CHEW treatment activated ROS and calcium signaling pathways in broccoli sprouts, which interacted through MAPK cascades.
- Taxon
- broccoliBrassica oleracea var. italicae4
- Treatment
- CHEW treatmente4
- Outcome
- ROS and calcium signaling transduction pathwayse4
- Qualifier
- interacted through MAPK cascadese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in broccoli sprouts”
e4“Comparative transcriptome and metabolome analyses revealed that CHEW treatment activated ROS and calcium signaling transduction pathways in broccoli sprouts and they interacted through MAPK cascades”
Claim · model-written summary
CHEW treatment promoted the biosynthesis of amino acids in broccoli sprouts.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Treatment
- CHEW treatmente5
- Outcome
- biosynthesis of amino acidse5
- Direction
- promotede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in broccoli sprouts”
e5“CHEW treatment not only promoted the biosynthesis of amino acids”
Claim · model-written summary
CHEW treatment enhanced the expression of structural genes in glucosinolate synthesis through transcription factors.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Treatment
- CHEW treatmente5
- Gene
- structural genese6
- Outcome
- expression of structural genes in glucosinolate synthesise6
- Direction
- enhancede6
- Qualifier
- through transcription factors (MYBs, bHLHs, WRKYs, etc.)e6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in broccoli sprouts”
e5“CHEW treatment not only promoted the biosynthesis of amino acids”
e6“but also enhanced the expression of structural genes in glucosinolate synthesis through transcription factors (MYBs, bHLHs, WRKYs, etc.)”
The same questions in other papers
calcium chloride dihydrate · nutrient: content · Brassica oleracea (wild cabbage)Results agree
1 paper in the same direction as this paper
- positive
calcium chloride dihydrate · molecular: metabolites · Brassica oleracea (wild cabbage)Results agree
1 paper in the same direction as this paper
- positive
calcium chloride dihydrate · quality: phytochemicals · Brassica oleracea (wild cabbage)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.