Abstract
Treatment with methyl jasmonate (MeJA) stimulates a multicomponent defense response in leaves and suspension-cultured cells of grapevine (Vitis vinifera L. cv. Limberger). MeJA induces development of necrotic lesions, similar to that normally associated with resistance to avirulent pathogens. Sustained exposure of both leaves and cell-suspension cultures to 50 μM MeJA provoked hypersensitive cell death, stimulated medium alkalinization accompanied by massive callose deposition, but did not induce accumulation of hydrogen peroxide from the oxidative burst. Transcripts of genes encoding diverse families of the pathogenesis-related proteins accumulated rapidly after MeJA application, followed by salicylic acid production. After several days systemic accumulation of a large number of defense-associated proteins, including pathogenesis-related proteins, peroxidase, cell wall extensin and enzymes involved in the phenylpropanoid biosynthetic pathway was induced. These cumulative results suggest that grapevine cells that perceived MeJA generated a cascade of events acting at both local and long distances, and causing the sequential and coordinated expression of specific defense responses with a timing and magnitude similar to the typical hypersensitive response against pathogens.
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Claims 8
Claim · model-written summary
Methyl jasmonate induced development of necrotic lesions in grapevine.
VitisNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MeJA induces development of necrotic lesions”
t0“Grapevine”
Claim · model-written summary
Sustained exposure of grapevine leaves and cell-suspension cultures to 50 μM MeJA provoked hypersensitive cell death.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sustained exposure of both leaves and cell-suspension cultures to 50 μM MeJA provoked hypersensitive cell death”
t0“Grapevine”
Claim · model-written summary
Sustained 50 μM MeJA exposure stimulated medium alkalinization accompanied by massive callose deposition in grapevine leaves and cell suspensions.
- Treatment
- 50 μM MeJAe0
- Outcome
- medium alkalinization accompanied by massive callose depositione0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sustained exposure of both leaves and cell-suspension cultures to 50 μM MeJA provoked hypersensitive cell death, stimulated medium alkalinization accompanied by massive callose deposition”
t0“Grapevine”
Claim · model-written summary
Sustained 50 μM MeJA exposure did not induce accumulation of hydrogen peroxide from the oxidative burst in grapevine leaves and cell suspensions.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sustained exposure of both leaves and cell-suspension cultures to 50 μM MeJA provoked hypersensitive cell death, stimulated medium alkalinization accompanied by massive callose deposition, but did not induce accumulation of hydrogen peroxide from the oxidative burst”
t0“Grapevine”
Claim · model-written summary
Transcripts of genes encoding diverse pathogenesis-related protein families accumulated rapidly after MeJA application in grapevine.
- Treatment
- MeJA applicatione0
- Outcome
- Transcripts of genes encoding diverse families of the pathogenesis-related proteinse0
- Direction
- accumulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcripts of genes encoding diverse families of the pathogenesis-related proteins accumulated rapidly after MeJA application”
t0“Grapevine”
Claim · model-written summary
Salicylic acid production followed the accumulation of pathogenesis-related transcripts after MeJA application in grapevine.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcripts of genes encoding diverse families of the pathogenesis-related proteins accumulated rapidly after MeJA application, followed by salicylic acid production”
t0“Grapevine”
Claim · model-written summary
After several days, MeJA induced systemic accumulation of many defense-associated proteins (pathogenesis-related proteins, peroxidase, cell wall extensin, phenylpropanoid pathway enzymes) in grapevine.
- Treatment
- MeJA applicatione1
- Outcome
- systemic accumulation of a large number of defense-associated proteinse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After several days systemic accumulation of a large number of defense-associated proteins, including pathogenesis-related proteins, peroxidase, cell wall extensin and enzymes involved in the phenylpropanoid biosynthetic pathway was induced”
e1“accumulated rapidly after MeJA application”
t0“Grapevine”
Claim · model-written summary
The authors conclude that grapevine cells perceiving MeJA generated a cascade of local and long-distance events causing sequential, coordinated expression of specific defense responses.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“grapevine cells that perceived MeJA generated a cascade of events acting at both local and long distances, and causing the sequential and coordinated expression of specific defense responses”
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.