Abstract
Abstract Plants grown in hydroponic culture make useful experimental models for soil‐grown plants. However, a major problem of nutrient solution culture is the adequate buffering of solution pH. Four methods of controlling pH in hydroponic culture of winter wheat ( Triticum aestivum L. ‘Centurk’) were compared at two levels of Ca (0.2 and 2.0 m M ) to determine the buffering method with the least effect on the uptake of essential nutrient elements. These methods were: (i) manual titration with NaOH; (ii) Amberlite DP‐1 weakly acidic cation exchange resin loaded with Ca, Mg, K, and H; (iii) the organic buffer, 2‐(N‐morpholino)ethanesulfonic acid (MES), at 1m M ; and (iv) MES a t 5 m M . Five‐millimolar MES gave the most consistent control of solution pH. However, it also inhibited Zn accumulation by wheat. One‐millimolar MES did not maintain the solution pH as well as 5 m M MES, but it did not significantly alter the uptake of any essential nutrient element. It is recommended as a pH buffer for the hydroponic culture of winter wheat. Resin in membrane bags did not adequately buffer the solution pH. It also removed some Mn and Zn from solution, thus depressing their uptake by wheat. Resin in a pump system adequately buffered the solution pH until its effectiveness was limited by the flow rate through the resin bed. Adequate buffering of solution pH was found to be critical for experiments that study the interactions between Ca and other nutrient elements. With the pH buffer, MES, the previously reported effects of Ca on depressing Mg, Mn, and Zn accumulation were observed. However, in the poorly buffered treatments, these elements tended to increase slightly with increasing Ca levels, perhaps due to the effect of Ca on maintaining root membrane integrity in an acidic environment.
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Claims 9
Claim · model-written summary
Five millimolar MES controlled solution pH most consistently.
Not reportedTaxonComparator
Verbatim
e0“Five‐millimolar MES gave the most consistent control of solution pH.”
Claim · model-written summary
Five millimolar MES inhibited wheat zinc accumulation.
- Taxon
- wheat
Triticum aestivume0 - Outcome
- Zn accumulation by wheate0
- Direction
- inhibitede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, it also inhibited Zn accumulation by wheat.”
Claim · model-written summary
One millimolar MES buffered solution pH less well than five millimolar MES.
Not reportedTaxon
Verbatim
e0“One‐millimolar MES did not maintain the solution pH as well as 5 m M MES”
Claim · model-written summary
One millimolar MES did not significantly alter essential nutrient uptake.
Not reportedTaxonTreatmentComparator
Verbatim
e0“it did not significantly alter the uptake of any essential nutrient element.”
Claim · model-written summary
Resin in membrane bags did not adequately buffer solution pH.
- Outcome
- buffer the solution pHe0
Not reportedTaxonComparatorValue
Verbatim
e0“Resin in membrane bags did not adequately buffer the solution pH.”
Claim · model-written summary
Membrane bag resin depressed wheat Mn and Zn uptake after removing these elements from solution.
- Taxon
- wheatTriticum aestivume0
- Outcome
- their uptake by wheate0
- Direction
- depressinge0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It also removed some Mn and Zn from solution, thus depressing their uptake by wheat.”
Claim · model-written summary
Pump system resin buffered pH adequately until flow rate limited effectiveness.
- Outcome
- buffered the solution pHe0
Not reportedTaxonComparatorValue
Verbatim
e0“Resin in a pump system adequately buffered the solution pH until its effectiveness was limited by the flow rate through the resin bed.”
Claim · model-written summary
With MES buffering, calcium depressed Mg, Mn and Zn accumulation.
Not reportedTaxonComparator
Verbatim
e0“With the pH buffer, MES, the previously reported effects of Ca on depressing Mg, Mn, and Zn accumulation were observed.”
Claim · model-written summary
Under poor buffering, these nutrient levels tended to rise slightly with calcium.
- Treatment
- increasing Ca levelse0
- Outcome
- these elementse0
- Direction
- increase slightlye0
- Qualifier
- poorly buffered treatmentse0
Not reportedTaxonComparator
Verbatim
e0“in the poorly buffered treatments, these elements tended to increase slightly with increasing Ca levels”
The same questions in other papers
calcium atom · nutrient: content · Triticum aestivum (wheat)Positive and negative results
3 papers in the same direction as this paper · 18 in the opposite direction
- positive
- positive
- positive
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See all 22 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.