Abstract
DNA binding and uptake by nuclei isolated from soybean (Glycine max L. Merr.) protoplasts were investigated using radioactive homogeneous DNA prepared from soybean cells. DNA binding to nuclei was found to decrease drastically with increased incubation time. Total uptake and acid-precipitable uptake reached a maximum after 20 minutes of incubation. Optimum DNA binding and uptake occurred at pH 6 and the process was enhanced by increasing the incubation temperature to 40 C. Salmonella typhimurium DNA and poly ([dA-dT]-[dA-dT]) competitively inhibited DNA binding whereas calf thymus DNA was less competitive; however, Micrococcus lysodeikticus DNA stimulated DNA binding and tobacco mosaic virus RNA had no effect. DNA binding and uptake was enhanced by addition of Mg ions, Ca ions, poly-l-lysine, and ATP. Increasing amounts of EDTA appeared to decrease DNA binding. Pronase strongly inhibited DNA binding and uptake.
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Claims 15
Claim · model-written summary
DNA binding decreased drastically with increased incubation time.
- Taxon
- soybean
Glycine maxe1 - Treatment
- increased incubation timee0
- Outcome
- DNA bindinge0
- Direction
- decrease drasticallye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA binding to nuclei was found to decrease drastically with increased incubation time”
e1“soybean (Glycine max L. Merr.) protoplasts”
Claim · model-written summary
Total and acid-precipitable DNA uptake peaked after 20 minutes.
- Taxon
- soybeanGlycine maxe1
- Outcome
- Total uptake and acid-precipitable uptakee0
- Direction
- maximume0
- Value
- 20 minutese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Total uptake and acid-precipitable uptake reached a maximum after 20 minutes of incubation”
e1“soybean (Glycine max L. Merr.) protoplasts”
Claim · model-written summary
DNA binding and uptake were optimum at pH 6.
- Taxon
- soybeanGlycine maxe1
- Outcome
- DNA binding and uptakee0
- Direction
- Optimume0
- Value
- pH 6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimum DNA binding and uptake occurred at pH 6”
e1“soybean (Glycine max L. Merr.) protoplasts”
Claim · model-written summary
Increasing incubation temperature to 40 C enhanced DNA binding and uptake.
- Taxon
- soybeanGlycine maxe1
- Treatment
- increasing the incubation temperature to 40 Ce0
- Outcome
- the processe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the process was enhanced by increasing the incubation temperature to 40 C”
e1“soybean (Glycine max L. Merr.) protoplasts”
Claim · model-written summary
Salmonella typhimurium DNA competitively inhibited DNA binding.
- Taxon
- soybeanGlycine maxe1
- Treatment
- Salmonella typhimurium DNAe0
- Outcome
- DNA bindinge0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Salmonella typhimurium DNA and poly ([dA-dT]-[dA-dT]) competitively inhibited DNA binding”
e1“soybean (Glycine max L. Merr.) protoplasts”
Claim · model-written summary
Poly ([dA-dT]-[dA-dT]) competitively inhibited DNA binding.
- Taxon
- soybeanGlycine maxe1
- Treatment
- poly ([dA-dT]-[dA-dT])e0
- Outcome
- DNA bindinge0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Salmonella typhimurium DNA and poly ([dA-dT]-[dA-dT]) competitively inhibited DNA binding”
e1“soybean (Glycine max L. Merr.) protoplasts”
Claim · model-written summary
Calf thymus DNA was less competitive in DNA binding.
- Taxon
- soybeanGlycine maxe1
- Treatment
- calf thymus DNAe0
- Outcome
- competitivee0
- Direction
- lesse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“calf thymus DNA was less competitive”
e1“soybean (Glycine max L. Merr.) protoplasts”
Claim · model-written summary
Micrococcus lysodeikticus DNA stimulated DNA binding.
- Taxon
- soybeanGlycine maxe1
- Treatment
- Micrococcus lysodeikticus DNAe0
- Outcome
- DNA bindinge0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Micrococcus lysodeikticus DNA stimulated DNA binding”
e1“soybean (Glycine max L. Merr.) protoplasts”
Claim · model-written summary
Tobacco mosaic virus RNA had no effect on DNA binding.
- Taxon
- soybeanGlycine maxe1
- Treatment
- tobacco mosaic virus RNAe0
- Outcome
- DNA bindinge2
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco mosaic virus RNA had no effect”
e1“soybean (Glycine max L. Merr.) protoplasts”
e2“Micrococcus lysodeikticus DNA stimulated DNA binding”
Claim · model-written summary
Mg ions enhanced DNA binding and uptake.
- Taxon
- soybeanGlycine maxe1
- Treatment
- Mg ionse0
- Outcome
- DNA binding and uptakee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA binding and uptake was enhanced by addition of Mg ions, Ca ions, poly-l-lysine, and ATP”
e1“soybean (Glycine max L. Merr.) protoplasts”
Claim · model-written summary
Ca ions enhanced DNA binding and uptake.
- Taxon
- soybeanGlycine maxe1
- Treatment
- Ca ionse0
- Outcome
- DNA binding and uptakee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA binding and uptake was enhanced by addition of Mg ions, Ca ions, poly-l-lysine, and ATP”
e1“soybean (Glycine max L. Merr.) protoplasts”
Claim · model-written summary
Poly-l-lysine enhanced DNA binding and uptake.
- Taxon
- soybeanGlycine maxe1
- Treatment
- poly-l-lysinee0
- Outcome
- DNA binding and uptakee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA binding and uptake was enhanced by addition of Mg ions, Ca ions, poly-l-lysine, and ATP”
e1“soybean (Glycine max L. Merr.) protoplasts”
Claim · model-written summary
ATP enhanced DNA binding and uptake.
- Taxon
- soybeanGlycine maxe1
- Treatment
- ATPe0
- Outcome
- DNA binding and uptakee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA binding and uptake was enhanced by addition of Mg ions, Ca ions, poly-l-lysine, and ATP”
e1“soybean (Glycine max L. Merr.) protoplasts”
Claim · model-written summary
Increasing EDTA appeared to decrease DNA binding.
- Taxon
- soybeanGlycine maxe1
- Treatment
- Increasing amounts of EDTAe0
- Outcome
- DNA bindinge0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing amounts of EDTA appeared to decrease DNA binding”
e1“soybean (Glycine max L. Merr.) protoplasts”
Claim · model-written summary
Pronase strongly inhibited DNA binding and uptake.
- Taxon
- soybeanGlycine maxe1
- Treatment
- Pronasee0
- Outcome
- DNA binding and uptakee0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pronase strongly inhibited DNA binding and uptake”
e1“soybean (Glycine max L. Merr.) protoplasts”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.