Abstract
The induction of nitrate reductase activity in root tips of cotton (Gossypium hirsutum L.) was regulated by several amino acids and by ammonium. Glycine, glutamine, and asparagine strongly inhibited induction of activity by nitrate and also decreased growth of sterile-cultured roots on a nitrate medium. Methionine, serine, and alanine weakly inhibited induction, and 11 other amino acids had little or no effect. Ammonium also decreased induction in root tips, but was most effective only at pH 7 or higher. The optimum conditions for ammonium regulation of induction were identical to those for growth of sterile-cultured roots on ammonium as the sole nitrogen source. Aspartate and glutamate strongly stimulated induction, but several lines of evidence indicated that the mechanism of this response was different from that elicited by the other amino acids. The effects of amino acids on induction appeared to be independent of nitrate uptake.In green shoot tissues, all attempts to demonstrate regulation of induction by amino acids failed. The great difference in observed responses of root and shoot to amino acids suggests that their nitrate reductase activities are regulated differently. Differential regulation of this enzyme is consistent with the responses of root and shoot nitrate reductase activity to nitrate.
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Claims 11
Claim · model-written summary
Glycine, glutamine and asparagine strongly inhibited nitrate reductase induction in cotton roots.
- Taxon
- cotton
Gossypium hirsutume1 - Organ / stage
- root tipse1
- Treatment
- Glycine, glutamine, and asparaginee0
- Outcome
- induction of activity by nitratee0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glycine, glutamine, and asparagine strongly inhibited induction of activity by nitrate”
e1“root tips of cotton (Gossypium hirsutum L.)”
Claim · model-written summary
Glycine, glutamine and asparagine decreased sterile cultured cotton root growth on nitrate medium.
- Taxon
- cottonGossypium hirsutume1
- Treatment
- Glycine, glutamine, and asparaginee0
- Outcome
- growth of sterile-cultured rootse0
- Direction
- decreasede0
- Setting
- nitrate mediume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glycine, glutamine, and asparagine strongly inhibited induction of activity by nitrate and also decreased growth of sterile-cultured roots on a nitrate medium”
e1“root tips of cotton (Gossypium hirsutum L.)”
Claim · model-written summary
Methionine, serine and alanine weakly inhibited induction.
- Taxon
- cottonGossypium hirsutume1
- Treatment
- Methionine, serine, and alaninee0
- Outcome
- inductione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Methionine, serine, and alanine weakly inhibited induction”
e1“root tips of cotton (Gossypium hirsutum L.)”
Claim · model-written summary
Eleven other amino acids had little or no effect on induction.
- Taxon
- cottonGossypium hirsutume2
- Treatment
- 11 other amino acidse0
- Outcome
- inductione1
- Direction
- little or no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“11 other amino acids had little or no effect”
e1“Methionine, serine, and alanine weakly inhibited induction, and 11 other amino acids had little or no effect”
e2“root tips of cotton (Gossypium hirsutum L.)”
Claim · model-written summary
Ammonium decreased nitrate reductase induction in root tips, most effectively at pH 7 or higher.
- Taxon
- cottonGossypium hirsutume1
- Organ / stage
- root tipse0
- Treatment
- Ammoniume0
- Outcome
- inductione0
- Direction
- decreasede0
- Qualifier
- pH 7 or highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ammonium also decreased induction in root tips, but was most effective only at pH 7 or higher”
e1“cotton (Gossypium hirsutum L.)”
Claim · model-written summary
Optimal ammonium regulation conditions matched conditions for root growth with ammonium as sole nitrogen source.
- Taxon
- cottonGossypium hirsutume1
- Outcome
- optimum conditions for ammonium regulation of inductione0
- Comparator
- growth of sterile-cultured roots on ammonium as the sole nitrogen sourcee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The optimum conditions for ammonium regulation of induction were identical to those for growth of sterile-cultured roots on ammonium as the sole nitrogen source”
e1“cotton (Gossypium hirsutum L.)”
Claim · model-written summary
Aspartate and glutamate strongly stimulated nitrate reductase induction.
- Taxon
- cottonGossypium hirsutume1
- Treatment
- Aspartate and glutamatee0
- Outcome
- inductione0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aspartate and glutamate strongly stimulated induction”
e1“cotton (Gossypium hirsutum L.)”
Claim · model-written summary
The authors infer that aspartate and glutamate act through a different regulatory mechanism from the other amino acids.
- Taxon
- cottonGossypium hirsutume2
- Treatment
- Aspartate and glutamatee1
- Outcome
- inductione1
- Qualifier
- mechanism of this response was differente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mechanism of this response was different from that elicited by the other amino acids”
e1“Aspartate and glutamate strongly stimulated induction”
e2“cotton (Gossypium hirsutum L.)”
Claim · model-written summary
Amino acid effects on induction appeared independent of nitrate uptake.
- Taxon
- cottonGossypium hirsutume1
- Treatment
- amino acidse0
- Outcome
- inductione0
- Qualifier
- independent of nitrate uptakee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effects of amino acids on induction appeared to be independent of nitrate uptake”
e1“cotton (Gossypium hirsutum L.)”
Claim · model-written summary
Amino acids did not demonstrably regulate induction in green shoots.
- Taxon
- cottonGossypium hirsutume1
- Organ / stage
- green shoot tissuese0
- Treatment
- amino acidse0
- Outcome
- regulation of inductione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In green shoot tissues, all attempts to demonstrate regulation of induction by amino acids failed”
e1“cotton (Gossypium hirsutum L.)”
Claim · model-written summary
The authors infer different regulation of nitrate reductase activities in roots and shoots.
- Taxon
- cottonGossypium hirsutume1
- Organ / stage
- root and shoote0
- Treatment
- amino acidse0
- Outcome
- nitrate reductase activities are regulated differentlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The great difference in observed responses of root and shoot to amino acids suggests that their nitrate reductase activities are regulated differently”
e1“cotton (Gossypium hirsutum L.)”
The same questions in other papers
glutamine · growth · Gossypium hirsutum (cotton)Positive and negative results
1 in the opposite direction
- positive
asparagine · molecular: expression · Gossypium hirsutum (cotton)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.