Abstract
A high efficiency shoot organogenesis from callus cultures and plant establishment protocol has been developed for cotton (Gossypium hirsutum L. cv SVPR-2) through shoot tip. After 6 weeks of culture, green-compact-nodular organogenic callus induction was observed on the medium fortified with (MS) basal salts, B5 vitamins, 2.0 mgl-1 of benzylaminopurine (BAP), 1.0 mgl-1 kinetin (KN), 30 gl-1 glucose and 8 gl-1 agar. The highest rate of shoot proliferation was achieved at 12th week and 8.6 shoots were produced per callus clump. Among the different concentrations tested media fortified with MS salts, B5 vitamins, 2 isopentynyl adenine(2-iP) (1.5 mgl-1), gibberellic acid (GA3) (0.6 mgl-1), glutamine (25 mgl-1) and glucose (30 gl-1) showed best response for multiple shoot proliferation from the induced callus. All the in vitro regenerated shoots were rooted on the medium fortified with MS salts, B5 vitamins, 30 gl-1 sucrose, 8 gl-1 agar and 1.5 mgl-1 of indole butyric acid (IBA). The regenerated plantlets, with tertiary roots are considered as matured plantlets and they were hardened on paper pots containing sand, soil and vermiculite in 1:1:1 ratio. The hardenedplants showed 85% survival rate and showed parental phenotypic characters.
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Claims 6
Claim · model-written summary
Green-compact-nodular organogenic callus was induced on medium with 2.0 mgl-1 BAP and 1.0 mgl-1 kinetin.
- Taxon
- Gossypium hirsutum
Gossypium hirsutumt0 - Material
- SVPR 2t0
- Organ / stage
- calluse1
- Treatment
- 2.0 mgl-1 of benzylaminopurine (BAP), 1.0 mgl-1 kinetin (KN)e1
- Outcome
- green-compact-nodular organogenic callus inductione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton (Gossypium hirsutum L. cv. SVPR 2)”
e1“green-compact-nodular organogenic callus induction was observed on the medium fortified with (MS) basal salts, B5 vitamins, 2.0 mgl-1 of benzylaminopurine (BAP), 1.0 mgl-1 kinetin (KN)”
Claim · model-written summary
The highest rate of shoot proliferation was reached at week 12, with 8.6 shoots per callus clump.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Material
- SVPR 2t0
- Organ / stage
- callus clumpe2
- Outcome
- shoot proliferatione2
- Direction
- highest ratee2
- Value
- 8.6 shootse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton (Gossypium hirsutum L. cv. SVPR 2)”
e2“The highest rate of shoot proliferation was achieved at 12th week and 8.6 shoots were produced per callus clump”
Claim · model-written summary
Multiple shoot proliferation from induced callus showed best response on medium with 2-iP, GA3, glutamine and glucose.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Material
- SVPR 2t0
- Treatment
- 2 isopentynyl adenine(2-iP) (1.5 mgl-1), gibberellic acid (GA3) (0.6 mgl-1), glutamine (25 mgl-1) and glucose (30 gl-1)e3
- Outcome
- multiple shoot proliferatione3
- Direction
- showed best responsee3
- Comparator
- different concentrations testede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton (Gossypium hirsutum L. cv. SVPR 2)”
e3“Among the different concentrations tested media fortified with MS salts, B5 vitamins, 2 isopentynyl adenine(2-iP) (1.5 mgl-1), gibberellic acid (GA3) (0.6 mgl-1), glutamine (25 mgl-1) and glucose (30 gl-1) showed best response for multiple shoot proliferation from the induced callus”
Claim · model-written summary
All in vitro regenerated shoots were rooted on medium with 1.5 mgl-1 indole butyric acid.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Material
- SVPR 2t0
- Treatment
- 1.5 mgl-1 of indole butyric acid (IBA)e4
- Outcome
- in vitro regenerated shoots were rootede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton (Gossypium hirsutum L. cv. SVPR 2)”
e4“All the in vitro regenerated shoots were rooted on the medium fortified with MS salts, B5 vitamins, 30 gl-1 sucrose, 8 gl-1 agar and 1.5 mgl-1 of indole butyric acid (IBA)”
Claim · model-written summary
Hardened regenerated plantlets had an 85% survival rate.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Material
- SVPR 2t0
- Outcome
- survival ratee5
- Value
- 85%e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton (Gossypium hirsutum L. cv. SVPR 2)”
e5“The hardenedplants showed 85% survival rate”
Claim · model-written summary
Hardened regenerated plantlets showed parental phenotypic characters.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Material
- SVPR 2t0
- Outcome
- phenotypic characterse6
- Direction
- parentale6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton (Gossypium hirsutum L. cv. SVPR 2)”
e6“showed parental phenotypic characters”
The same questions in other papers
glutamine · growth · Gossypium hirsutum (cotton)Positive and negative results
1 in the opposite direction
- negative
glucose · growth · Gossypium hirsutum (cotton)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.