Abstract
In cotton, factors such as carbon sources, hormonal combination, and type of explant influence the responses to callus induction. In this study callus induction was optimized to get high-frequency callus in cotton. Different carbon sources were tested, and among them, glucose at 3% was found to be the best carbohydrate in callus induction. Culture medium supplemented with 2,4-D (0.1 mg.L-1) and KIN (0.5 mg.L-1) gave a high callogenesis response, unlike other hormonal combinations. With explants, hypocotyl (72.93%) appeared easier to induce callus than cotyledon (48.75%) following root (37.5%). So, carbone sources, hormone, and their concentration affect significantly callus induction. The highest frequency of callus induction was then observed when hypocotyl explants were cultured on medium supplemented with 0.1 mg.L-1 of 2,4-D in combination with 0.5 mg.L-1 of KIN and 3% glucose. The induced-callus obtained were friable and of high quality. The other types of combinations resulted in the formation of compact callus with sometimes roots emerging from it. The present study may provide an efficient protocol to improve the callogenesis responses by adjusting the carbohydrates, the hormonal regime, and the type of explant to be cultured for a high-frequency callus induction of the Y331B-R5 cotton cultivar.
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Claims 8
Claim · model-written summary
Glucose at 3% was the best carbohydrate for cotton callus induction.
- Taxon
- cotton
Gossypium hirsutumt0 - Treatment
- glucose at 3%e0
- Outcome
- callus inductione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glucose at 3% was found to be the best carbohydrate in callus induction.”
t0“Cotton”
Claim · model-written summary
Medium with 2,4-D and KIN gave high callogenesis relative to other hormone combinations.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- 2,4-D (0.1 mg.L-1) and KIN (0.5 mg.L-1)e0
- Outcome
- callogenesis responsee0
- Direction
- highe0
- Comparator
- other hormonal combinationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Culture medium supplemented with 2,4-D (0.1 mg.L-1) and KIN (0.5 mg.L-1) gave a high callogenesis response, unlike other hormonal combinations.”
t0“Cotton”
Claim · model-written summary
Hypocotyl explants had 72.93% callus induction, above cotyledon and root explants.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- hypocotyle0
- Treatment
- hypocotyle0
- Outcome
- induce calluse0
- Direction
- easiere0
- Value
- 72.93%e0
- Comparator
- cotyledone0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hypocotyl (72.93%) appeared easier to induce callus than cotyledon (48.75%) following root (37.5%).”
t0“Cotton”
Claim · model-written summary
Cotyledon explants had 48.75% callus induction.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- cotyledone0
- Outcome
- cotyledone0
- Value
- 48.75%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cotyledon (48.75%) following root (37.5%).”
t0“Cotton”
Claim · model-written summary
Root explants had 37.5% callus induction.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- roote0
- Outcome
- roote0
- Value
- 37.5%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root (37.5%).”
t0“Cotton”
Claim · model-written summary
The combined hypocotyl, 2,4-D, KIN and glucose treatment gave the highest callus induction frequency.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- hypocotyle0
- Treatment
- 0.1 mg.L-1 of 2,4-D in combination with 0.5 mg.L-1 of KIN and 3% glucosee0
- Outcome
- frequency of callus inductione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest frequency of callus induction was then observed when hypocotyl explants were cultured on medium supplemented with 0.1 mg.L-1 of 2,4-D in combination with 0.5 mg.L-1 of KIN and 3% glucose.”
t0“Cotton”
Claim · model-written summary
The induced callus was friable and high quality.
- Taxon
- cottonGossypium hirsutumt0
- Outcome
- friable and of high qualitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The induced-callus obtained were friable and of high quality.”
t0“Cotton”
Claim · model-written summary
Other treatment combinations formed compact callus, sometimes with roots.
- Taxon
- cottonGossypium hirsutumt0
- Outcome
- formation of compact callus with sometimes roots emerging from ite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The other types of combinations resulted in the formation of compact callus with sometimes roots emerging from it.”
t0“Cotton”
The same questions in other papers
2,4-D · growth · Gossypium hirsutum (cotton)Positive and negative results
11 papers in the same direction as this paper · 8 in the opposite direction
- positive
- negative
- no effect
16 papers more in this group
See all 20 papers →glucose · growth · Gossypium hirsutum (cotton)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.