Abstract
Arabidopsis and other Brassicaceae produce an enormous diversity of aliphatic glucosinolates, a group of methionine (Met)-derived plant secondary compounds containing a beta-thio-glucose moiety, a sulfonated oxime, and a variable side chain. We fine-scale mapped GSL-ELONG, a locus controlling variation in the side-chain length of aliphatic glucosinolates. Within this locus, a polymorphic gene was identified that determines whether Met is extended predominantly by either one or by two methylene groups to produce aliphatic glucosinolates with either three- or four-carbon side chains. Two allelic mutants deficient in four-carbon side-chain glucosinolates were shown to contain independent missense mutations within this gene. In cell-free enzyme assays, a heterologously expressed cDNA from this locus was capable of condensing 2-oxo-4-methylthiobutanoic acid with acetyl-coenzyme A, the initial reaction in Met chain elongation. The gene methylthioalkylmalate synthase1 (MAM1) is a member of a gene family sharing approximately 60% amino acid sequence similarity with 2-isopropylmalate synthase, an enzyme of leucine biosynthesis that condenses 2-oxo-3-methylbutanoate with acetyl-coenzyme A.
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Claims 5
Claim · model-written summary
GSL-ELONG was fine-scale mapped as a locus controlling aliphatic glucosinolate side-chain length.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- GSL-ELONGe0
- Outcome
- variation in the side-chain length of aliphatic glucosinolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We fine-scale mapped GSL-ELONG, a locus controlling variation in the side-chain length of aliphatic glucosinolates.”
t0“Arabidopsis”
Claim · model-written summary
A polymorphic gene determines whether methionine is extended by one or two methylene groups, yielding three- or four-carbon side chains.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- a polymorphic genee0
- Outcome
- aliphatic glucosinolates with either three- or four-carbon side chainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a polymorphic gene was identified that determines whether Met is extended predominantly by either one or by two methylene groups to produce aliphatic glucosinolates with either three- or four-carbon side chains”
t0“Arabidopsis”
Claim · model-written summary
Two allelic mutants deficient in four-carbon glucosinolates carried independent missense mutations in this gene.
- Taxon
- ArabidopsisArabidopsist0
- Material
- Two allelic mutantse0
- Outcome
- independent missense mutationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two allelic mutants deficient in four-carbon side-chain glucosinolates were shown to contain independent missense mutations within this gene.”
t0“Arabidopsis”
Claim · model-written summary
A heterologously expressed cDNA from the locus catalyzed condensation of 2-oxo-4-methylthiobutanoic acid with acetyl-coenzyme A.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- a heterologously expressed cDNAe0
- Outcome
- condensing 2-oxo-4-methylthiobutanoic acid with acetyl-coenzyme Ae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a heterologously expressed cDNA from this locus was capable of condensing 2-oxo-4-methylthiobutanoic acid with acetyl-coenzyme A”
t0“Arabidopsis”
Claim · model-written summary
MAM1 belongs to a gene family with approximately 60% amino acid sequence similarity to 2-isopropylmalate synthase.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- methylthioalkylmalate synthase1 (MAM1)e0
- Outcome
- amino acid sequence similarity with 2-isopropylmalate synthasee0
- Value
- approximately 60%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The gene methylthioalkylmalate synthase1 (MAM1) is a member of a gene family sharing approximately 60% amino acid sequence similarity with 2-isopropylmalate synthase”
t0“Arabidopsis”