Abstract
The soybean vegetative storage protein genes vspA and vspB are highly expressed in developing leaves, stems, flowers, and pods as compared with roots, seeds, and mature leaves and stems. In this paper, we report that physiological levels of methyl jasmonate (MeJA) and soluble sugars synergistically stimulate accumulation of vsp mRNAs. Treatment of excised mature soybean (Glycine max Merr. cv Williams) leaves with 0.2 molar sucrose and 10 micromolar MeJA caused a large accumulation of vsp mRNAs, whereas little accumulation occurred when these compounds were supplied separately. In soybean cell suspension cultures, the synergistic effect of sucrose and MeJA on the accumulation of vspB mRNA was maximal at 58 millimolar sucrose and was observed with fructose or glucose substituted for sucrose. In dark-grown soybean seedlings, the highest levels of vsp mRNAs occurred in the hypocotyl hook, which also contained high levels of MeJA and soluble sugars. Lower levels of vsp mRNAs, MeJA, and soluble sugars were found in the cotyledons, roots, and nongrowing regions of the stem. Wounding of mature soybean leaves induced a large accumulation of vsp mRNAs when wounded plants were incubated in the light. Wounded plants kept in the dark or illuminated plants sprayed with dichlorophenyldimethylurea, an inhibitor of photosynthetic electron transport, showed a greatly reduced accumulation of vsp mRNAs. The time courses for the accumulation of vsp mRNAs induced by wounding or sucrose/MeJA treatment were similar. These results strongly suggest that vsp expression is coregulated by endogenous levels of MeJA (or jasmonic acid) and soluble carbohydrate during normal vegetative development and in wounded leaves.
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Claims 10
Claim · model-written summary
Soybean vspA and vspB are more highly expressed in developing leaves, stems, flowers and pods than in roots, seeds and mature tissues.
- Taxon
- soybean
Glycine maxe0 - Organ / stage
- developing leaves, stems, flowers, and podse0
- Gene
- vspA and vspBe0
- Outcome
- highly expressede0
- Direction
- highlye0
- Comparator
- roots, seeds, and mature leaves and stemse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean vegetative storage protein genes vspA and vspB are highly expressed in developing leaves, stems, flowers, and pods as compared with roots, seeds, and mature leaves and stems”
Claim · model-written summary
Methyl jasmonate and soluble sugars synergistically stimulated vsp mRNA accumulation.
- Taxon
- soybeanGlycine maxt0
- Treatment
- methyl jasmonate (MeJA) and soluble sugarse0
- Outcome
- accumulation of vsp mRNAse0
- Direction
- stimulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“physiological levels of methyl jasmonate (MeJA) and soluble sugars synergistically stimulate accumulation of vsp mRNAs”
t0“Soybean”
Claim · model-written summary
Combined sucrose and MeJA caused large vsp mRNA accumulation whereas separate treatments caused little accumulation.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 0.2 molar sucrose and 10 micromolar MeJAe0
- Outcome
- accumulation of vsp mRNAse0
- Direction
- largee0
- Comparator
- these compounds were supplied separatelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“0.2 molar sucrose and 10 micromolar MeJA caused a large accumulation of vsp mRNAs, whereas little accumulation occurred when these compounds were supplied separately”
t0“Soybean”
Claim · model-written summary
In soybean cell cultures, the synergistic vspB response peaked at 58 millimolar sucrose and persisted with fructose or glucose substitution.
- Taxon
- soybeanGlycine maxt0
- Gene
- vspBe0
- Outcome
- accumulation of vspB mRNAe0
- Direction
- maximale0
- Value
- 58 millimolar sucrosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“synergistic effect of sucrose and MeJA on the accumulation of vspB mRNA was maximal at 58 millimolar sucrose and was observed with fructose or glucose substituted for sucrose”
t0“Soybean”
Claim · model-written summary
Dark-grown soybean hypocotyl hooks had the highest vsp mRNA, MeJA and soluble sugar levels.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- hypocotyl hooke0
- Outcome
- levels of vsp mRNAse0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“highest levels of vsp mRNAs occurred in the hypocotyl hook, which also contained high levels of MeJA and soluble sugars”
t0“Soybean”
Claim · model-written summary
Cotyledons, roots and nongrowing stem regions had lower vsp mRNA, MeJA and soluble sugar levels.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- cotyledons, roots, and nongrowing regions of the steme0
- Outcome
- levels of vsp mRNAs, MeJA, and soluble sugarse0
- Direction
- Lowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lower levels of vsp mRNAs, MeJA, and soluble sugars were found in the cotyledons, roots, and nongrowing regions of the stem”
t0“Soybean”
Claim · model-written summary
Wounding soybean leaves in light induced large vsp mRNA accumulation.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Wounding of mature soybean leavese0
- Outcome
- accumulation of vsp mRNAse0
- Direction
- largee0
- Qualifier
- incubated in the lighte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wounding of mature soybean leaves induced a large accumulation of vsp mRNAs when wounded plants were incubated in the light”
Claim · model-written summary
Dark incubation reduced wound-induced vsp mRNA accumulation.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- kept in the darke0
- Outcome
- accumulation of vsp mRNAse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wounded plants kept in the dark or illuminated plants sprayed with dichlorophenyldimethylurea, an inhibitor of photosynthetic electron transport, showed a greatly reduced accumulation of vsp mRNAs”
t0“Soybean”
Claim · model-written summary
Dichlorophenyldimethylurea treatment reduced vsp mRNA accumulation in illuminated wounded plants.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- sprayed with dichlorophenyldimethylureae0
- Outcome
- accumulation of vsp mRNAse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“illuminated plants sprayed with dichlorophenyldimethylurea, an inhibitor of photosynthetic electron transport, showed a greatly reduced accumulation of vsp mRNAs”
t0“Soybean”
Claim · model-written summary
Wounding and sucrose/MeJA treatment induced similar time courses of vsp mRNA accumulation.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- time courses for the accumulation of vsp mRNAse0
- Direction
- similare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“time courses for the accumulation of vsp mRNAs induced by wounding or sucrose/MeJA treatment were similar”
t0“Soybean”
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