Abstract
The hypoxic stress response of Zea mays L. seedling roots involves regulation of gene expression at transcriptional and posttranscriptional levels. We investigated the effect of hypoxia on the translational machinery of seedling roots. The levels of monoribosomes and ribosomal subunits increased dramatically within 1 hour of stress. Prolonged hypoxia resulted in continued accumulation of nontranslating ribosomes, as well as increased levels of small polyribosomes. The return of seedlings to normal aerobic conditions resulted in recovery of normal polyribosome levels. Comparison of ribosomal proteins from control and hypoxic roots revealed differences in quantity and electrophoretic mobility. In vivo labeling of roots with [(35)S]methionine revealed variations in newly synthesized ribosomal proteins. In vivo labeling of roots with [(32)P]orthophosphate revealed a major reduction in the phosphorylation of a 31 kilodalton ribosomal protein in hypoxic stressed roots. In vitro phosphorylation of ribosomal proteins by endogenous kinases was used to probe for differences in ribosome structure and composition. The patterns of in vitro kinased phosphoproteins of ribosomes from control and hypoxic roots were not identical. Variation in phosphoproteins of polyribosomes from control and hypoxic roots, as well as among polyribosomes from hypoxic roots were observed. These results indicate that modification of the translational machinery occurs in response to hypoxic stress.
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Claims 8
Claim · model-written summary
Hypoxia dramatically increased monoribosomes and ribosomal subunits within one hour.
- Treatment
- stresse0
- Outcome
- monoribosomes and ribosomal subunitse0
- Direction
- increasede0
- Qualifier
- within 1 houre0
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The levels of monoribosomes and ribosomal subunits increased dramatically within 1 hour of stress”
t0“Maize”
Claim · model-written summary
Prolonged hypoxia accumulated nontranslating ribosomes and increased small polyribosomes.
- Treatment
- Prolonged hypoxiae0
- Outcome
- nontranslating ribosomes, as well as increased levels of small polyribosomese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Prolonged hypoxia resulted in continued accumulation of nontranslating ribosomes, as well as increased levels of small polyribosomes”
t0“Maize”
Claim · model-written summary
Returning seedlings to aerobic conditions restored normal polyribosome levels.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The return of seedlings to normal aerobic conditions resulted in recovery of normal polyribosome levels”
t0“Maize”
Claim · model-written summary
Ribosomal proteins differed in quantity and electrophoretic mobility between control and hypoxic roots.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Comparison of ribosomal proteins from control and hypoxic roots revealed differences in quantity and electrophoretic mobility”
t0“Maize”
Claim · model-written summary
Newly synthesized ribosomal proteins varied in hypoxic roots.
- Outcome
- variations in newly synthesized ribosomal proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vivo labeling of roots with [(35)S]methionine revealed variations in newly synthesized ribosomal proteins”
t0“Maize”
Claim · model-written summary
Hypoxic stress markedly reduced phosphorylation of a 31 kilodalton ribosomal protein.
- Treatment
- hypoxic stressed rootse0
- Gene
- 31 kilodalton ribosomal proteine0
- Outcome
- phosphorylation of a 31 kilodalton ribosomal proteine0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a major reduction in the phosphorylation of a 31 kilodalton ribosomal protein in hypoxic stressed roots”
t0“Maize”
Claim · model-written summary
In vitro phosphoprotein patterns differed between ribosomes of control and hypoxic roots.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The patterns of in vitro kinased phosphoproteins of ribosomes from control and hypoxic roots were not identical”
t0“Maize”
Claim · model-written summary
Polyribosome phosphoproteins varied between control and hypoxic roots and among hypoxic roots.
- Outcome
- Variation in phosphoproteins of polyribosomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Variation in phosphoproteins of polyribosomes from control and hypoxic roots, as well as among polyribosomes from hypoxic roots were observed”
t0“Maize”
The same questions in other papers
flooding · molecular: expression · Zea mays (maize)Positive and negative results
3 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- positive
- positive
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.