Abstract
SUMMARY The nodule occupancy of white clover cv. Milkanova three months after inoculation in an upland soil with a 1:1 mixture of an effective (CP3B) and an ineffective strain (R4) of R. trifolii , differed significantly depending on the lime level. The percentage of nodules occupied by the effective strain decreased from 64% to 29% with increasing lime level whilst the ineffective strain nodules increased from 4% to 24% with mixed nodule infections also increasing from 9% to 22% There was no such difference after 14 months with this cultivar nor with cv. S184 at either harvest. Inoculation with a higher ratio of the less preferred strain (R4) resulted in this strain dominating the nodules and significantly reducing the dry weight of the plants (first harvest) and clover cover of plots (second harvest). Where antibiotic resistant mutants of these strains were used, with one exception, there were no significant differences between the wild‐type strains and their mutant derivatives for any of the parameters measured. In a comparison of the two marker techniques used, fluorescent ELISA was less sensitive than the antibiotic resistance technique for the identification of the occupants of small ineffective nodules. The use of both techniques in large field experiments is discussed. Clover establishment was affected by lime level during the establishment phase but the beneficial effect of lime had disappeared by the second harvest year.
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Claims 12
Claim · model-written summary
Nodule occupancy of white clover cv. Milkanova three months after inoculation with a 1:1 mixture of effective strain CP3B and ineffective strain R4 of R. trifolii differed significantly with lime level.
- Taxon
- white clover
Trifolium repenst0 - Material
- cv. Milkanovae1
- Treatment
- lime levele1
- Interactor
- R. trifolii
Rhizobium leguminosarume1symbiont - Outcome
- nodule occupancye1
- Setting
- upland soile1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“white clover”
e1“The nodule occupancy of white clover cv. Milkanova three months after inoculation in an upland soil with a 1:1 mixture of an effective (CP3B) and an ineffective strain (R4) of R. trifolii , differed significantly depending on the lime level”
Claim · model-written summary
The percentage of nodules occupied by the effective strain decreased from 64% to 29% with increasing lime level.
- Taxon
- white cloverTrifolium repenst0
- Treatment
- increasing lime levele1
- Interactor
- effective straine1symbiont
- Outcome
- percentage of nodules occupied by the effective straine1
- Direction
- decreasede1
- Value
- 64% to 29%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“white clover”
e1“The percentage of nodules occupied by the effective strain decreased from 64% to 29% with increasing lime level”
Claim · model-written summary
Nodules occupied by the ineffective strain increased from 4% to 24% with increasing lime level.
- Taxon
- white cloverTrifolium repenst0
- Treatment
- increasing lime levele1
- Interactor
- ineffective straine2symbiont
- Outcome
- ineffective strain nodulese2
- Direction
- increasede2
- Value
- 4% to 24%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“white clover”
e1“with increasing lime level”
e2“the ineffective strain nodules increased from 4% to 24%”
Claim · model-written summary
Mixed nodule infections increased from 9% to 22% with increasing lime level.
- Taxon
- white cloverTrifolium repenst0
- Treatment
- increasing lime levele1
- Outcome
- mixed nodule infectionse2
- Direction
- increasinge2
- Value
- 9% to 22%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“white clover”
e1“with increasing lime level”
e2“mixed nodule infections also increasing from 9% to 22%”
Claim · model-written summary
No such difference in nodule occupancy by lime level was found after 14 months with cv. Milkanova, nor with cv. S184 at either harvest.
- Taxon
- white cloverTrifolium repenst0
- Material
- cv. S184e2
- Treatment
- lime levele1
- Outcome
- no such differencee2
- Direction
- no such differencee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“white clover”
e1“depending on the lime level”
e2“There was no such difference after 14 months with this cultivar nor with cv. S184 at either harvest”
Claim · model-written summary
Inoculation with a higher ratio of the less preferred strain R4 resulted in this strain dominating the nodules.
- Taxon
- white cloverTrifolium repenst0
- Treatment
- Inoculation with a higher ratio of the less preferred strain (R4)e1
- Interactor
- less preferred strain (R4)e1symbiont
- Outcome
- dominating the nodulese1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“white clover”
e1“Inoculation with a higher ratio of the less preferred strain (R4) resulted in this strain dominating the nodules”
Claim · model-written summary
Inoculation with a higher ratio of the less preferred strain R4 significantly reduced plant dry weight at the first harvest.
- Taxon
- white cloverTrifolium repenst0
- Treatment
- Inoculation with a higher ratio of the less preferred strain (R4)e1
- Interactor
- less preferred strain (R4)e1symbiont
- Outcome
- dry weight of the plantse1
- Direction
- reducinge1
- Qualifier
- first harveste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“white clover”
e1“Inoculation with a higher ratio of the less preferred strain (R4) resulted in this strain dominating the nodules and significantly reducing the dry weight of the plants (first harvest)”
Claim · model-written summary
Inoculation with a higher ratio of the less preferred strain R4 significantly reduced clover cover of plots at the second harvest.
- Taxon
- white cloverTrifolium repenst0
- Treatment
- Inoculation with a higher ratio of the less preferred strain (R4)e1
- Interactor
- less preferred strain (R4)e1symbiont
- Outcome
- clover cover of plotse1
- Direction
- reducinge1
- Qualifier
- second harveste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“white clover”
e1“Inoculation with a higher ratio of the less preferred strain (R4) resulted in this strain dominating the nodules and significantly reducing the dry weight of the plants (first harvest) and clover cover of plots (second harvest)”
Claim · model-written summary
Antibiotic resistant mutants of the strains showed, with one exception, no significant differences from the wild-type strains for any parameter measured.
- Taxon
- white cloverTrifolium repenst0
- Treatment
- antibiotic resistant mutantse1
- Outcome
- parameters measurede1
- Direction
- no significant differencese1
- Qualifier
- with one exceptione1
- Comparator
- wild‐type strainse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“white clover”
e1“Where antibiotic resistant mutants of these strains were used, with one exception, there were no significant differences between the wild‐type strains and their mutant derivatives for any of the parameters measured”
Claim · model-written summary
Fluorescent ELISA was less sensitive than the antibiotic resistance technique for identifying the occupants of small ineffective nodules.
Verbatim
e1“fluorescent ELISA was less sensitive than the antibiotic resistance technique for the identification of the occupants of small ineffective nodules”
Claim · model-written summary
Clover establishment was affected by lime level during the establishment phase.
- Taxon
- white cloverTrifolium repenst0
- Treatment
- lime levele1
- Outcome
- Clover establishmente1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“white clover”
e1“Clover establishment was affected by lime level during the establishment phase”
Claim · model-written summary
The beneficial effect of lime on clover had disappeared by the second harvest year.
- Taxon
- white cloverTrifolium repenst0
- Treatment
- limee1
- Outcome
- beneficial effecte1
- Direction
- disappearede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“white clover”
e1“the beneficial effect of lime had disappeared by the second harvest year”
The same questions in other papers
lime · growth · Trifolium repens (white clover)Results differ
9 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- no effect
- no effect
10 papers more in this group
See all 14 papers →inoculation · growth · Trifolium repens (white clover)Results differ
4 papers in the same direction as this paper · 31 in the opposite direction
- positive
- positive
- positive
32 papers more in this group
See all 36 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.