Abstract
The effect of potato plant ( Solanum tuberosum L., cv. Desnitsa) transformation with the desA gene from Synechocystis sp. PCC 6803, encoding Δ12 acyl-lipid desaturase, on the development of plant tolerance to oxidative stress was studied. To initiate oxidative stress, plants were treated with 1 mM paraquat; this treatment enhanced oxidative processes in both wild-type and transformed potato plants via the activation of superoxide anion-radical generation. This resulted in the activated oxidation of membrane lipids and the formation of a great amount of fatty acids with coupled double bonds (conjugated dienes, CD), further breakdown of lipid molecules, and enhanced production of MDA in tissues of wild-type and transformed plants. The characteristics of oxidative stress, including lipid peroxidation, were less pronounced in transformants as compared with wild-type plants. After treatment with paraquat, activities of main antioxidant enzymes (superoxide dismutase, catalase, and peroxidase) were much higher in wild-type than in transformed plants. Thus, expression of inserted heterologous desA gene for Δ12 acyl-lipid desaturase in potato plants resulted in improved tolerance of transformants to oxidative stress due to the more efficient maintenance of stable cell membrane structure functioning, and this permits prevention of electron “jump” to oxygen and, as a result, of accelerated ROS generation. More developed and regularly arranged chloroplast membrane system in transformants may also favor their improved tolerance.
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Claims 4
Claim · model-written summary
Paraquat enhanced oxidative processes in wild-type and transformed potato.
- Taxon
- potato
Solanum tuberosume0 - Treatment
- 1 mM paraquate0
- Outcome
- oxidative processese0
- Direction
- enhancede0
- Qualifier
- activation of superoxide anion-radical generatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“To initiate oxidative stress, plants were treated with 1 mM paraquat; this treatment enhanced oxidative processes in both wild-type and transformed potato plants via the activation of superoxide anion-radical generation.”
Claim · model-written summary
Oxidative stress and lipid peroxidation were less pronounced in transformants than wild-type potato.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- transformantse0
- Outcome
- oxidative stress, including lipid peroxidatione0
- Direction
- less pronouncede0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The characteristics of oxidative stress, including lipid peroxidation, were less pronounced in transformants as compared with wild-type plants.”
t0“potato”
Claim · model-written summary
Paraquat-treated wild-type potato had higher antioxidant enzyme activities than transformants.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- paraquate0
- Outcome
- activities of main antioxidant enzymes (superoxide dismutase, catalase, and peroxidase)e0
- Direction
- much highere0
- Comparator
- transformed plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After treatment with paraquat, activities of main antioxidant enzymes (superoxide dismutase, catalase, and peroxidase) were much higher in wild-type than in transformed plants.”
t0“potato”
Claim · model-written summary
desA expression improved potato tolerance to oxidative stress.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- expression of inserted heterologous desA genee0
- Gene
- desAe0
- Outcome
- tolerance of transformants to oxidative stresse0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of inserted heterologous desA gene for Δ12 acyl-lipid desaturase in potato plants resulted in improved tolerance of transformants to oxidative stress”
The same questions in other papers
transgenic · physiology: oxidative · Solanum tuberosum (potato)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- positive
- negative
- negative
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.