Abstract
MicroRNAs (miRNAs) are small noncoding RNAs in eukaryotes. Plant endogenous miRNAs play pivotal roles in regulating plant development and defense responses. MicroRNA394 (miR394) has been reported to regulate plant development, abiotic stresses and defense responses. Previous reports showed that miR394 responded to P. infestans inoculation in potato, indicating that miR394 may be involved in defense responses. In this study, we further investigated its role in potato defense against P. infestans. Stable expression of miR394 in tobacco and potato enhances the susceptibility to P. infestans, which is accompanied with the reduced accumulation of ROS and down-regulation of the PTI (pattern-triggered immunity) marker genes. Besides well-known target StLCR, miR394 also targets StA/N-INVE, which encodes a chloroplast Alkaline/Neutral Invertases (A/N-INVE). Both StLCR and StA/N-INVE positively regulate late blight resistance, while miR394 degrades them. Interestingly, StA/N-INVE is located in the chloroplast, indicating that miR394 may manipulate chloroplast immunity. Degradation of StA/N-INVE may affect the chloroplast function and hence lead to the compromised ROS (reactive oxygen species) burst and reduced retrograde signaling from the chloroplast to the nucleus and cytoplasm. In summary, this study provides new information that miR394 targets and degrades StA/N-INVE and StLCR, which are positive regulators, to enhance potato susceptibility to P. infestans.
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Claims 10
Claim · model-written summary
Stable miR394 expression increased tobacco susceptibility to P. infestans.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- Stable expression of miR394e0
- Interactor
- P. infestanse0pathogen
- Gene
- miR394e0
- Outcome
- susceptibility to P. infestanse0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stable expression of miR394 in tobacco and potato enhances the susceptibility to P. infestans”
Claim · model-written summary
Stable miR394 expression increased potato susceptibility to P. infestans.
- Taxon
- potato
Solanum tuberosume0 - Treatment
- Stable expression of miR394e0
- Interactor
- P. infestanse0pathogen
- Gene
- miR394e0
- Outcome
- susceptibility to P. infestanse0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stable expression of miR394 in tobacco and potato enhances the susceptibility to P. infestans”
Claim · model-written summary
Stable miR394 expression reduced ROS accumulation during P. infestans susceptibility.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- Stable expression of miR394e0
- Interactor
- P. infestanse0pathogen
- Gene
- miR394e0
- Outcome
- accumulation of ROSe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stable expression of miR394 in tobacco and potato enhances the susceptibility to P. infestans, which is accompanied with the reduced accumulation of ROS”
t0“Potato”
Claim · model-written summary
Stable miR394 expression downregulated PTI marker genes.
- Taxon
- PotatoSolanum tuberosumt0
- Treatment
- Stable expression of miR394e0
- Interactor
- P. infestanse0pathogen
- Gene
- miR394e0
- Outcome
- PTI (pattern-triggered immunity) marker genese0
- Direction
- down-regulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stable expression of miR394 in tobacco and potato enhances the susceptibility to P. infestans, which is accompanied with the reduced accumulation of ROS and down-regulation of the PTI (pattern-triggered immunity) marker genes”
t0“Potato”
Claim · model-written summary
miR394 targets StA/N-INVE.
- Taxon
- PotatoSolanum tuberosumt0
- Gene
- StA/N-INVEe0
- Outcome
- miR394 also targets StA/N-INVEe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR394 also targets StA/N-INVE”
t0“Potato”
Claim · model-written summary
StLCR positively regulates late blight resistance.
- Taxon
- PotatoSolanum tuberosumt0
- Gene
- StLCRe0
- Outcome
- late blight resistancee0
- Direction
- positively regulatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both StLCR and StA/N-INVE positively regulate late blight resistance, while miR394 degrades them”
t0“Potato”
Claim · model-written summary
StA/N-INVE positively regulates late blight resistance.
- Taxon
- PotatoSolanum tuberosumt0
- Gene
- StA/N-INVEe0
- Outcome
- late blight resistancee0
- Direction
- positively regulatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both StLCR and StA/N-INVE positively regulate late blight resistance, while miR394 degrades them”
t0“Potato”
Claim · model-written summary
miR394 degrades StLCR and StA/N-INVE.
- Taxon
- PotatoSolanum tuberosumt0
- Gene
- miR394e0
- Outcome
- degrades theme0
- Qualifier
- StLCR and StA/N-INVEe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both StLCR and StA/N-INVE positively regulate late blight resistance, while miR394 degrades them”
t0“Potato”
Claim · model-written summary
StA/N-INVE is located in the chloroplast.
- Taxon
- PotatoSolanum tuberosumt0
- Gene
- StA/N-INVEe0
- Outcome
- located in the chloroplaste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StA/N-INVE is located in the chloroplast”
t0“Potato”
Claim · model-written summary
The authors propose StA/N-INVE degradation may compromise ROS burst and chloroplast retrograde signaling.
- Taxon
- PotatoSolanum tuberosumt0
- Gene
- StA/N-INVEe0
- Outcome
- compromised ROS (reactive oxygen species) burst and reduced retrograde signalinge0
- Qualifier
- Degradation of StA/N-INVEe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Degradation of StA/N-INVE may affect the chloroplast function and hence lead to the compromised ROS (reactive oxygen species) burst and reduced retrograde signaling from the chloroplast to the nucleus and cytoplasm”
t0“Potato”
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See all 46 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.