Abstract
Low temperature induces a number of genes that encode the proteins promoting tolerance to freezing, mediated by ABA-dependent and ABA-independent pathways in plants. The cis-acting element called C/DRE is known to respond to low temperature independently of ABA action. To investigate the signalling and network of ABA-independent pathways, the transgenic Arabidopsis plants were generated containing several copies of the C/DRE derived from cor15a gene with a minimal promoter fused to a GUS reporter gene. The transgenic plants containing four copies of the C/DRE (4C/DRE-GUS) showed responsiveness to cold and drought treatments and were used for characterization of cold signalling and cross-talk. Cold-induced GUS expression was inhibited by okadaic acid at 1 nM, indicating that protein phosphatase 2A might act as a positive regulator. Light was shown to activate cold- and drought-induced GUS expression. Photo-reversibility of the GUS mRNA by red and far-red light with concomitant cold treatment suggests a role of phytochrome as a photoreceptor in mediating light signalling to activate the cold-induced gene expression through the C/DRE. Furthermore, GUS expression analysis in phyA or phyB or phyAphyB mutant backgrounds showed that phytochrome B is a primary photoreceptor responsible for the activation of cold-stress signalling in response to light. Light enhanced the induction kinetics of CBF1, 2, and 3 encoding the cognate transcription factors, and cor15a, in a consecutive manner compared to the dark condition in the cold, suggesting that the connection point between cold and light signalling mediated by phytochrome is at a higher step than the expression of CBF genes.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
The C/DRE reporter responded to cold and drought.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Treatment
- cold and drought treatmentse0
- Outcome
- responsivenesse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic plants containing four copies of the C/DRE (4C/DRE-GUS) showed responsiveness to cold and drought treatments”
t0“Arabidopsis thaliana”
Claim · model-written summary
Okadaic acid inhibited cold-induced GUS expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- okadaic acid at 1 nMe0
- Outcome
- Cold-induced GUS expressione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cold-induced GUS expression was inhibited by okadaic acid at 1 nM”
t0“Arabidopsis thaliana”
Claim · model-written summary
Light activated cold- and drought-induced GUS expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Lighte0
- Outcome
- cold- and drought-induced GUS expressione0
- Direction
- activatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Light was shown to activate cold- and drought-induced GUS expression.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Photoreversibility implicated phytochrome in cold-induced expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- phytochromee0
- Outcome
- cold-induced gene expression through the C/DREe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Photo-reversibility of the GUS mRNA by red and far-red light with concomitant cold treatment suggests a role of phytochrome as a photoreceptor in mediating light signalling to activate the cold-induced gene expression through the C/DRE.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Mutant analysis identified phytochrome B as the primary cold-signalling photoreceptor.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- phytochrome Be0
- Outcome
- activation of cold-stress signalling in response to lighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GUS expression analysis in phyA or phyB or phyAphyB mutant backgrounds showed that phytochrome B is a primary photoreceptor responsible for the activation of cold-stress signalling in response to light.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Light enhanced induction kinetics of CBF genes and cor15a relative to dark in cold.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Lighte0
- Outcome
- induction kinetics of CBF1, 2, and 3 encoding the cognate transcription factors, and cor15ae0
- Direction
- enhancede0
- Comparator
- dark conditione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Light enhanced the induction kinetics of CBF1, 2, and 3 encoding the cognate transcription factors, and cor15a, in a consecutive manner compared to the dark condition in the cold”
t0“Arabidopsis thaliana”
The same questions in other papers
light · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
31 papers in the same direction as this paper · 15 in the opposite direction
- negative
- no effect
- negative
43 papers more in this group
See all 47 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.