Abstract
Plastidial phosphoglucomutase (PGM) plays an important role in starch synthesis and degradation. Nonetheless, the impact of enhanced plastidial PGM activity on metabolism in photosynthetic tissue is yet to be elucidated. In this study, we generated transplastomic tobacco plants overproducing Arabidopsis thaliana plastidial PGM (AtptPGM) in chloroplasts and analyzed the consequent metabolic and physiological parameters in the transplastomic plants. AtptPGM accumulated in the chloroplasts to up to 16% of total soluble protein in the leaves. PGM activity in leaves increased 100-fold relative to that of wild-type plants. The transplastomic plants were phenotypically indistinguishable in their growth rates, photosynthetic activities, and starch synthesis from wild-type plants, but hexose partitioning in the light period was dramatically different. Furthermore, alteration of extracellular invertase activity was observed in the lower leaves of the transplastomic plants. These observations suggest that high-level expression of plastidial PGM alters hexose partitioning in light periods via modification of extracellular invertase activity.
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Claims 6
Claim · model-written summary
AtptPGM accumulated to up to 16% of total soluble protein in leaves.
- Taxon
- tobacco
Nicotiana tabacumt0 - Organ / stage
- leavese0
- Gene
- AtptPGMe0
- Outcome
- total soluble proteine0
- Value
- 16%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtptPGM accumulated in the chloroplasts to up to 16% of total soluble protein in the leaves.”
t0“Tobacco Chloroplasts”
Claim · model-written summary
Leaf PGM activity increased 100-fold relative to wild type.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- leavese0
- Treatment
- AtptPGMe1
- Gene
- PGMe0
- Outcome
- PGM activitye0
- Direction
- increasede0
- Value
- 100-folde0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PGM activity in leaves increased 100-fold relative to that of wild-type plants.”
t0“Tobacco Chloroplasts”
e1“transplastomic tobacco plants overproducing Arabidopsis thaliana plastidial PGM (AtptPGM) in chloroplasts”
Claim · model-written summary
Transplastomic plants had similar growth rates, photosynthetic activities and starch synthesis to wild type.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- transplastomic plantse0
- Outcome
- growth rates, photosynthetic activities, and starch synthesise0
- Direction
- phenotypically indistinguishablee0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transplastomic plants were phenotypically indistinguishable in their growth rates, photosynthetic activities, and starch synthesis from wild-type plants”
t0“Tobacco Chloroplasts”
Claim · model-written summary
Transplastomic plants had dramatically different light-period hexose partitioning.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- transplastomic plantse1
- Outcome
- hexose partitioninge0
- Direction
- dramatically differente0
- Qualifier
- in the light periode0
- Comparator
- wild-type plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“hexose partitioning in the light period was dramatically different.”
t0“Tobacco Chloroplasts”
e1“The transplastomic plants were phenotypically indistinguishable in their growth rates, photosynthetic activities, and starch synthesis from wild-type plants, but hexose partitioning in the light period was dramatically different.”
Claim · model-written summary
Extracellular invertase activity was altered in lower leaves of transplastomic plants.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- lower leavese0
- Gene
- extracellular invertasee0
- Outcome
- alteration of extracellular invertase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“alteration of extracellular invertase activity was observed in the lower leaves of the transplastomic plants.”
t0“Tobacco Chloroplasts”
Claim · model-written summary
Authors infer that high plastidial PGM expression alters light-period hexose partitioning through extracellular invertase activity.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- plastidial PGMe0
- Outcome
- hexose partitioninge0
- Qualifier
- via modification of extracellular invertase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high-level expression of plastidial PGM alters hexose partitioning in light periods via modification of extracellular invertase activity.”
t0“Tobacco Chloroplasts”