Paper · 1992 · FEBS Letters

ATPase state and activity in thylakoids from normal and water‐stressed lupin

S Meyer; Y de Kouchkovsky

7
claims
14
verbatim quotes
9
citations
1
research areas
—
Not open access

Abstract

To compare with the case of phosphorylation, ATP hydrolysis was investigated in thylakoids from plants submitted to drought. For medium stresses the relationship between hydrolysis rate and deactivation constant of the de-energized enzyme when delta pH varies is unchanged, hence the basic mechanisms are preserved; (PS1-driven) energization than is neither affected, at any light intensity. As drought intensifies, hydrolysis and monogalactolipids fall somewhat in parallel, whereas the deactivation constant changes with digalactolipids and phosphatidic acid content. This alteration of the F0 lipid environment must be transduced to F1, the subunits of which, however, are preserved, as shown by LDS-PAGE. ATP synthesis (thiol-oxidized enzyme) and hydrolysis (thiol-reduced enzyme) show an identical decrease with stress. Thus the latter impairs a common early step, probably the activation of the oxidized enzyme.

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Claims 7

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Relationship between ATP hydrolysis rate and deactivation constant was unchanged under medium stresses

Taxon
lupinLupinust0
Treatment
medium stressese0
Outcome
relationship between hydrolysis rate and deactivation constante0
Direction
unchangede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lupinus
Lupinus · Taxon · picture of the genus (one of its species)Jean-Claude Echardour [CC BY-SA], via Tela Botanica · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

t0

“lupin”

✓ located verbatim in the source · title, characters 71–76 · show in text ↑
e0

“For medium stresses the relationship between hydrolysis rate and deactivation constant of the de-energized enzyme when delta pH varies is unchanged”

✓ located verbatim in the source · abstract, characters 125–272 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Energization of thylakoids was not affected at any light intensity

Taxon
lupinLupinust0
Treatment
any light intensitye1
Outcome
energizatione1
Direction
neither affectede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lupinus
Lupinus · Taxon · picture of the genus (one of its species)Jean-Claude Echardour [CC BY-SA], via Tela Botanica · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

t0

“lupin”

✓ located verbatim in the source · title, characters 71–76 · show in text ↑
e1

“(PS1-driven) energization than is neither affected, at any light intensity”

✓ located verbatim in the source · abstract, characters 316–390 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Hydrolysis and monogalactolipids fell somewhat in parallel as drought intensified

Taxon
lupinLupinust0
Treatment
droughte2
Compound
monogalactolipidse2
Outcome
hydrolysise2
Direction
falle2
Qualifier
somewhat in parallele2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lupinus
Lupinus · Taxon · picture of the genus (one of its species)Jean-Claude Echardour [CC BY-SA], via Tela Botanica · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

t0

“lupin”

✓ located verbatim in the source · title, characters 71–76 · show in text ↑
e2

“As drought intensifies, hydrolysis and monogalactolipids fall somewhat in parallel”

✓ located verbatim in the source · abstract, characters 392–474 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Deactivation constant changes with digalactolipids and phosphatidic acid content

Taxon
lupinLupinust0
Compound
digalactolipids and phosphatidic acid contente3
Outcome
deactivation constante3
Direction
changese3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lupinus
Lupinus · Taxon · picture of the genus (one of its species)Jean-Claude Echardour [CC BY-SA], via Tela Botanica · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

t0

“lupin”

✓ located verbatim in the source · title, characters 71–76 · show in text ↑
e3

“whereas the deactivation constant changes with digalactolipids and phosphatidic acid content”

✓ located verbatim in the source · abstract, characters 476–568 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

Thylakoid F1 subunits were preserved under drought

Taxon
lupinLupinust0
Gene
F1e4
Outcome
subunitse4
Direction
preservede4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lupinus
Lupinus · Taxon · picture of the genus (one of its species)Jean-Claude Echardour [CC BY-SA], via Tela Botanica · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

t0

“lupin”

✓ located verbatim in the source · title, characters 71–76 · show in text ↑
e4

“This alteration of the F0 lipid environment must be transduced to F1, the subunits of which, however, are preserved, as shown by LDS-PAGE”

✓ located verbatim in the source · abstract, characters 570–707 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

ATP synthesis and hydrolysis decreased identically with stress

Taxon
lupinLupinust0
Treatment
stresse5
Outcome
ATP synthesis (thiol-oxidized enzyme) and hydrolysis (thiol-reduced enzyme)e5
Direction
identical decreasee5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lupinus
Lupinus · Taxon · picture of the genus (one of its species)Jean-Claude Echardour [CC BY-SA], via Tela Botanica · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

t0

“lupin”

✓ located verbatim in the source · title, characters 71–76 · show in text ↑
e5

“ATP synthesis (thiol-oxidized enzyme) and hydrolysis (thiol-reduced enzyme) show an identical decrease with stress”

✓ located verbatim in the source · abstract, characters 709–823 · show in text ↑
Mechanismhypothesisoriginal resultmeasured

Claim · model-written summary

Stress probably impairs a common early step, the activation of the oxidized enzyme

Taxon
lupinLupinust0
Outcome
common early stepe6
Qualifier
activation of the oxidized enzymee6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lupinus
Lupinus · Taxon · picture of the genus (one of its species)Jean-Claude Echardour [CC BY-SA], via Tela Botanica · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

t0

“lupin”

✓ located verbatim in the source · title, characters 71–76 · show in text ↑
e6

“Thus the latter impairs a common early step, probably the activation of the oxidized enzyme”

✓ located verbatim in the source · abstract, characters 825–916 · show in text ↑