Abstract
Photosynthesis is one of the biological processes most sensitive to heat stress in plants. Carbon assimilation, which depends on ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), is one of the major sites sensitive to heat stress in photosynthesis. In this study, the roles of a tomato (Solanum lycopersicum) chloroplast-targeted DnaJ protein (SlCDJ2) in resisting heat using sense and antisense transgenic tomatoes were examined. SlCDJ2 was found to be uniformly distributed in the thylakoids and stroma of the chloroplasts. Under heat stress, sense plants exhibited higher chlorophyll contents and fresh weights, and lower accumulation of reactive oxygen species (ROS) and membrane damage. Moreover, Rubisco activity, Rubisco large subunit (RbcL) content, and CO2 assimilation capacity were all higher in sense plants and lower in antisense plants compared with wild-type plants. Thus, SlCDJ2 contributes to maintenance of CO2 assimilation capacity mainly by protecting Rubisco activity under heat stress. SlCDJ2 probably achieves this by keeping the levels of proteolytic enzymes low, which prevents accelerated degradation of Rubisco under heat stress. Furthermore, a chloroplast heat-shock protein 70 was identified as a binding partner of SlCDJ2 in yeast two-hybrid assays. Taken together, these findings establish a role for SlCDJ2 in maintaining Rubisco activity in plants under heat stress.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
SlCDJ2 is uniformly distributed in the thylakoids and stroma of chloroplasts.
- Taxon
- tomato
Solanum lycopersicume0 - Organ / stage
- thylakoids and stroma of the chloroplastse1
- Gene
- SlCDJ2e1
- Outcome
- uniformly distributede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a tomato (Solanum lycopersicum) chloroplast-targeted DnaJ protein (SlCDJ2)”
e1“SlCDJ2 was found to be uniformly distributed in the thylakoids and stroma of the chloroplasts”
Claim · model-written summary
Under heat stress, sense plants had higher chlorophyll contents and fresh weights.
- Taxon
- tomatoSolanum lycopersicume0
- Material
- sense plantse2
- Treatment
- heat stresse2
- Outcome
- chlorophyll contents and fresh weightse2
- Direction
- highere2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a tomato (Solanum lycopersicum) chloroplast-targeted DnaJ protein (SlCDJ2)”
e2“Under heat stress, sense plants exhibited higher chlorophyll contents and fresh weights, and lower accumulation of reactive oxygen species (ROS) and membrane damage”
Claim · model-written summary
Under heat stress, sense plants had lower accumulation of reactive oxygen species and membrane damage.
- Taxon
- tomatoSolanum lycopersicume0
- Material
- sense plantse2
- Treatment
- heat stresse2
- Outcome
- accumulation of reactive oxygen species (ROS) and membrane damagee2
- Direction
- lowere2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a tomato (Solanum lycopersicum) chloroplast-targeted DnaJ protein (SlCDJ2)”
e2“Under heat stress, sense plants exhibited higher chlorophyll contents and fresh weights, and lower accumulation of reactive oxygen species (ROS) and membrane damage”
Claim · model-written summary
Sense plants had higher Rubisco activity, RbcL content and CO2 assimilation capacity than wild-type plants.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- sense plantse4
- Outcome
- Rubisco activity, Rubisco large subunit (RbcL) content, and CO2 assimilation capacitye4
- Direction
- highere4
- Comparator
- wild-type plantse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a tomato (Solanum lycopersicum) chloroplast-targeted DnaJ protein (SlCDJ2)”
e4“Rubisco activity, Rubisco large subunit (RbcL) content, and CO2 assimilation capacity were all higher in sense plants and lower in antisense plants compared with wild-type plants”
Claim · model-written summary
Antisense plants had lower Rubisco activity, RbcL content and CO2 assimilation capacity than wild-type plants.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- antisense plantse4
- Outcome
- Rubisco activity, Rubisco large subunit (RbcL) content, and CO2 assimilation capacitye4
- Direction
- lowere4
- Comparator
- wild-type plantse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a tomato (Solanum lycopersicum) chloroplast-targeted DnaJ protein (SlCDJ2)”
e4“Rubisco activity, Rubisco large subunit (RbcL) content, and CO2 assimilation capacity were all higher in sense plants and lower in antisense plants compared with wild-type plants”
Claim · model-written summary
SlCDJ2 contributes to maintenance of CO2 assimilation capacity by protecting Rubisco activity under heat stress.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- SlCDJ2e5
- Outcome
- maintenance of CO2 assimilation capacitye5
- Qualifier
- protecting Rubisco activity under heat stresse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a tomato (Solanum lycopersicum) chloroplast-targeted DnaJ protein (SlCDJ2)”
e5“SlCDJ2 contributes to maintenance of CO2 assimilation capacity mainly by protecting Rubisco activity under heat stress”
Claim · model-written summary
SlCDJ2 probably keeps proteolytic enzyme levels low, preventing accelerated Rubisco degradation under heat stress.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- SlCDJ2e6
- Outcome
- accelerated degradation of Rubiscoe6
- Qualifier
- keeping the levels of proteolytic enzymes lowe6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a tomato (Solanum lycopersicum) chloroplast-targeted DnaJ protein (SlCDJ2)”
e6“SlCDJ2 probably achieves this by keeping the levels of proteolytic enzymes low, which prevents accelerated degradation of Rubisco under heat stress”
Claim · model-written summary
A chloroplast heat-shock protein 70 binds SlCDJ2 in yeast two-hybrid assays.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- chloroplast heat-shock protein 70e7
- Outcome
- binding partner of SlCDJ2e7
- Setting
- yeast two-hybrid assayse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a tomato (Solanum lycopersicum) chloroplast-targeted DnaJ protein (SlCDJ2)”
e7“a chloroplast heat-shock protein 70 was identified as a binding partner of SlCDJ2 in yeast two-hybrid assays”
The same questions in other papers
heat · physiology: oxidative · Solanum lycopersicum (tomato)Results differ
5 papers in the same direction as this paper · 13 in the opposite direction
- negative
- negative
- negative
15 papers more in this group
See all 19 papers →heat · physiology: pigments · Solanum lycopersicum (tomato)Results differ
7 papers in the same direction as this paper · 12 in the opposite direction
- negative
- no effect
- negative
16 papers more in this group
See all 20 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.