Abstract
Fusarium verticillioides (teleomorph Gibberella moniliformis) and F. graminearum (teleomorph G. zeae) are well known to cause devastating diseases on cereal crops. Despite their importance, our understanding of the molecular mechanisms involved in these host-pathogen interactions is limited. The FSR1 locus in F. verticillioides was identified by screening REMI mutants for loss of virulence in maize stalk rot inoculation studies. FSR1 encodes an 823-codon open reading frame interrupted by two introns. The Fsr1 protein shares 60% sequence identity with the Sordaria macrospora Pro11, a multimodular protein with four putative protein-protein binding domains (caveolin-binding domain, coiled-coil structure, calmodulin-binding motif, and seven-WD40 repeats), which plays a regulatory role in cell differentiation and ascocarp development. Our data demonstrate that FSR1 is essential for female fertility and virulence in F. verticillioides. Significantly, targeted disruption of the FSR1 ortholog in F. graminearum (FgFSR1) reduced virulence on barley and deterred perithecia formation. Cross-complementation experiments demonstrated that the gene function is conserved in the two Fusarium species. FSR1 is expressed constitutively, and we hypothesize that Fsr1 regulates virulence by acting as a scaffold for a signal transduction pathway. A survey of available genome databases indicates Fsr1 homologs are present in a number of filamentous fungi and animal systems but not in budding yeast or plants. A maximum likelihood analysis of this gene family reveals well-supported monophyletic clades associated with fungi and animals.
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Claims 4
Claim · model-written summary
FSR1 was identified in a screen for loss of virulence in maize stalk rot.
- Interactor
- F. verticillioides
Fusarium verticillioidese0pathogen - Gene
- FSR1e0
- Outcome
- loss of virulence in maize stalk rote0
Zea maysNot reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The FSR1 locus in F. verticillioides was identified by screening REMI mutants for loss of virulence in maize stalk rot inoculation studies.”
Claim · model-written summary
FSR1 is essential for F. verticillioides virulence.
- Interactor
- F. verticillioidesFusarium verticillioidese0pathogen
- Gene
- FSR1e0
- Outcome
- virulence in F. verticillioidese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our data demonstrate that FSR1 is essential for female fertility and virulence in F. verticillioides.”
e1“The FSR1 locus in F. verticillioides was identified by screening REMI mutants for loss of virulence in maize stalk rot inoculation studies.”
Claim · model-written summary
Disruption of FgFSR1 reduced F. graminearum virulence on barley.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Treatment
- targeted disruption of the FSR1 orthologe0
- Interactor
- F. graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume0pathogen - Gene
- FgFSR1e0
- Outcome
- virulence on barleye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e0“targeted disruption of the FSR1 ortholog in F. graminearum (FgFSR1) reduced virulence on barley and deterred perithecia formation.”
Claim · model-written summary
Disruption of FgFSR1 deterred perithecia formation.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- targeted disruption of the FSR1 orthologe0
- Interactor
- F. graminearumFusarium graminearume0pathogen
- Gene
- FgFSR1e0
- Outcome
- perithecia formatione0
- Direction
- deterrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“targeted disruption of the FSR1 ortholog in F. graminearum (FgFSR1) reduced virulence on barley and deterred perithecia formation.”
The same questions in other papers
loss of function · Fusarium graminearum · Hordeum vulgare (barley)Positive and negative results
2 papers in the same direction as this paper · 5 in the opposite direction
- negative
- no effect
- no effect
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.