Abstract
In Arabidopsis, genes encoding functional enzymes for the synthesis and degradation of trehalose have been detected recently. In this study we analyzed how trehalose affects the metabolism and development of Arabidopsis seedlings. Exogenously applied trehalose (25 mM) strongly reduced the elongation of the roots and, concomitantly, induced a strong accumulation of starch in the shoots, whereas the contents of soluble sugars were not increased. When Arabidopsis seedlings were grown on trehalose plus sucrose (Suc), root elongation was restored, but starch still accumulated to a much larger extent than during growth on Suc alone. The accumulation of starch in the shoots of trehalose-treated seedlings was accompanied by an increased activity of ADP-glucose pyrophosphorylase and an induction of the expression of the ADP-glucose pyrophosphorylase gene, ApL3. Even in the presence of 50 mM Suc, which itself also slightly induced ApL3, trehalose (5 mM) led to a further increase in ApL3 expression. These results suggest that trehalose interferes with carbon allocation to the sink tissues by inducing starch synthesis in the source tissues. Furthermore, trehalose induced the expression of the beta-amylase gene, AT-beta-Amy, in combination with Suc but not when trehalose was supplied alone, indicating that trehalose can modulate sugar-mediated gene expression.
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Claims 12
Claim · model-written summary
Exogenous trehalose reduced root elongation.
- Taxon
- Arabidopsis
Arabidopsise1 - Treatment
- trehalose (25 mM)e0
- Outcome
- elongation of the rootse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenously applied trehalose (25 mM) strongly reduced the elongation of the roots”
e1“development of Arabidopsis seedlings”
Claim · model-written summary
Exogenous trehalose induced starch accumulation in shoots.
- Taxon
- ArabidopsisArabidopsise1
- Organ / stage
- shootse0
- Treatment
- trehalose (25 mM)e0
- Outcome
- accumulation of starche0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenously applied trehalose (25 mM) strongly reduced the elongation of the roots and, concomitantly, induced a strong accumulation of starch in the shoots”
e1“development of Arabidopsis seedlings”
Claim · model-written summary
Trehalose did not increase soluble sugar contents.
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- trehalose (25 mM)e1
- Outcome
- contents of soluble sugarse0
- Direction
- not increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“whereas the contents of soluble sugars were not increased”
e1“Exogenously applied trehalose (25 mM)”
e2“development of Arabidopsis seedlings”
Claim · model-written summary
Sucrose with trehalose restored root elongation.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- trehalose plus sucrose (Suc)e0
- Outcome
- root elongatione0
- Direction
- restorede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When Arabidopsis seedlings were grown on trehalose plus sucrose (Suc), root elongation was restored”
Claim · model-written summary
Trehalose plus sucrose produced more starch than sucrose alone.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- trehalose plus sucrose (Suc)e0
- Outcome
- starche0
- Direction
- much larger extente0
- Comparator
- Suc alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When Arabidopsis seedlings were grown on trehalose plus sucrose (Suc), root elongation was restored, but starch still accumulated to a much larger extent than during growth on Suc alone”
Claim · model-written summary
Trehalose-treated seedlings showed increased ADP-glucose pyrophosphorylase activity.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- trehalose-treatede0
- Gene
- ADP-glucose pyrophosphorylasee0
- Outcome
- activity of ADP-glucose pyrophosphorylasee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The accumulation of starch in the shoots of trehalose-treated seedlings was accompanied by an increased activity of ADP-glucose pyrophosphorylase”
e1“development of Arabidopsis seedlings”
Claim · model-written summary
Trehalose induced ApL3 expression.
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- trehalose-treatede1
- Gene
- ApL3e0
- Outcome
- expression of the ADP-glucose pyrophosphorylase gene, ApL3e0
- Direction
- inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an induction of the expression of the ADP-glucose pyrophosphorylase gene, ApL3”
e1“trehalose-treated seedlings”
e2“development of Arabidopsis seedlings”
Claim · model-written summary
Sucrose slightly induced ApL3 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“50 mM Suc, which itself also slightly induced ApL3”
e1“development of Arabidopsis seedlings”
Claim · model-written summary
Trehalose further increased ApL3 expression in the presence of sucrose.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- trehalose (5 mM)e0
- Gene
- ApL3e0
- Outcome
- ApL3 expressione0
- Direction
- increasee0
- Comparator
- 50 mM Suce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Even in the presence of 50 mM Suc, which itself also slightly induced ApL3, trehalose (5 mM) led to a further increase in ApL3 expression”
e1“development of Arabidopsis seedlings”
Claim · model-written summary
Trehalose induced AT-beta-Amy expression with sucrose.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- trehalosee0
- Gene
- AT-beta-Amye0
- Outcome
- expression of the beta-amylase gene, AT-beta-Amye0
- Direction
- inducede0
- Qualifier
- in combination with Suce0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“trehalose induced the expression of the beta-amylase gene, AT-beta-Amy, in combination with Suc”
e1“development of Arabidopsis seedlings”
Claim · model-written summary
Trehalose alone did not induce AT-beta-Amy expression.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- trehalosee0
- Gene
- AT-beta-Amye0
- Outcome
- expression of the beta-amylase gene, AT-beta-Amye0
- Direction
- not when trehalose was supplied alonee0
- Comparator
- in combination with Suce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“trehalose induced the expression of the beta-amylase gene, AT-beta-Amy, in combination with Suc but not when trehalose was supplied alone”
e1“development of Arabidopsis seedlings”
Claim · model-written summary
The authors suggest trehalose interferes with carbon allocation by inducing source-tissue starch synthesis.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- trehalosee0
- Outcome
- carbon allocation to the sink tissuese0
- Qualifier
- inducing starch synthesis in the source tissuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results suggest that trehalose interferes with carbon allocation to the sink tissues by inducing starch synthesis in the source tissues”
e1“development of Arabidopsis seedlings”
The same questions in other papers
trehalose · growth · ArabidopsisPositive and negative results
1 in the opposite direction
- negative
sucrose · growth · ArabidopsisPositive and negative results
8 papers in the same direction as this paper · 8 in the opposite direction
- positive
- negative
- negative
13 papers more in this group
See all 17 papers →trehalose · molecular: expression · ArabidopsisResults agree
2 papers in the same direction as this paper
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.