Abstract
Trehalose is a non-reducing sugar that plays an important role in plant growth and development. To study the role of trehalose on lipid metabolism and gluconeogenesis, Arabidopsis thaliana wild type (WT) and TreF (a line expressing trehalase) were grown on ½ MS medium with or without 100 mM sucrose and or trehalose in light or continuous darkness. In dark, trehalose leads skotomorphogenesis in WT seedlings and inhibits hypocotyl elongation without altering root growth. Then, a knock out mutant of icl (defective in isocitrate lyase/ICL) was identified in the SALK T-DNA insertion line, yet plants of this line were not altered with regard to growth on MS medium supplemented with or without trehalose in light condition, compared to WT. But the hypocotyl length of icl seedling was shorter than WT when grown on trehalose in darkness. The current data revealed that trehalose feeding altered seedling establishment in both WT and icl mutant. ICL enzyme activity measurement showed that the patterns of changes were similar in all treatments. Meanwhile, trehalose feeding reduced icl gene expression and enzyme activity. Trehalose fed seedlings demonstrated a high accumulation of total lipids in darkness. Also fatty acids level was higher in seedlings grown in darkness, compared with the light condition. Therefore, trehalose may inhibit lipid utilization by suppressing icl gene expression and enzyme activity and thus restrict the supply of carbon sources to the growing seedling. These observations confirm that trehalose regulates plant metabolism in both light and dark.
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Claims 12
Claim · model-written summary
In darkness, trehalose induced skotomorphogenesis in WT seedlings.
- Taxon
- Arabidopsis
Arabidopsist0 - Material
- WT seedlingse0
- Treatment
- trehalosee0
- Outcome
- skotomorphogenesise0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In dark, trehalose leads skotomorphogenesis in WT seedlings”
t0“Arabidopsis”
Claim · model-written summary
In darkness, trehalose inhibited hypocotyl elongation in WT seedlings.
- Taxon
- ArabidopsisArabidopsist0
- Material
- WT seedlingse0
- Treatment
- trehalosee0
- Outcome
- hypocotyl elongatione0
- Direction
- inhibitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In dark, trehalose leads skotomorphogenesis in WT seedlings and inhibits hypocotyl elongation without altering root growth”
t0“Arabidopsis”
Claim · model-written summary
In darkness, trehalose did not alter WT root growth.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- trehalosee0
- Outcome
- root growthe0
- Direction
- without alteringe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In dark, trehalose leads skotomorphogenesis in WT seedlings and inhibits hypocotyl elongation without altering root growth”
t0“Arabidopsis”
Claim · model-written summary
The identified icl knockout grew similarly to WT on MS medium with or without trehalose in light.
- Taxon
- ArabidopsisArabidopsist0
- Material
- iclt0
- Treatment
- MS medium supplemented with or without trehalose in light conditione0
- Outcome
- growthe0
- Direction
- not alterede0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plants of this line were not altered with regard to growth on MS medium supplemented with or without trehalose in light condition, compared to WT”
t0“Arabidopsis icl Mutant”
Claim · model-written summary
On trehalose in darkness, icl seedlings had shorter hypocotyls than WT.
- Taxon
- ArabidopsisArabidopsist0
- Material
- icl seedlinge0
- Treatment
- trehalose in darknesse0
- Outcome
- hypocotyl lengthe0
- Direction
- shortere0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the hypocotyl length of icl seedling was shorter than WT when grown on trehalose in darkness”
t0“Arabidopsis”
Claim · model-written summary
Trehalose feeding altered seedling establishment in both WT and icl.
- Taxon
- ArabidopsisArabidopsist0
- Material
- WT and icl mutante0
- Treatment
- trehalose feedinge0
- Outcome
- seedling establishmente0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“trehalose feeding altered seedling establishment in both WT and icl mutant”
t0“Arabidopsis”
Claim · model-written summary
ICL enzyme activity showed similar change patterns across treatments.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ICLe0
- Outcome
- patterns of changese0
- Direction
- similare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ICL enzyme activity measurement showed that the patterns of changes were similar in all treatments”
t0“Arabidopsis”
Claim · model-written summary
Trehalose feeding reduced icl gene expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- trehalose feedinge0
- Gene
- icle0
- Outcome
- icl gene expressione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“trehalose feeding reduced icl gene expression and enzyme activity”
t0“Arabidopsis”
Claim · model-written summary
Trehalose feeding reduced ICL enzyme activity.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- trehalose feedinge0
- Gene
- icle0
- Outcome
- enzyme activitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“trehalose feeding reduced icl gene expression and enzyme activity”
t0“Arabidopsis”
Claim · model-written summary
Trehalose-fed seedlings accumulated high total lipids in darkness.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Trehalosee0
- Outcome
- total lipidse0
- Direction
- highe0
- Qualifier
- in darknesse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Trehalose fed seedlings demonstrated a high accumulation of total lipids in darkness”
t0“Arabidopsis”
Claim · model-written summary
Seedlings in darkness had higher fatty acid levels than those in light.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- darknesse0
- Outcome
- fatty acids levele0
- Direction
- highere0
- Comparator
- light conditione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fatty acids level was higher in seedlings grown in darkness, compared with the light condition”
t0“Arabidopsis”
Claim · model-written summary
Authors suggest trehalose suppresses icl expression and activity, inhibiting lipid utilization and carbon supply.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- trehalosee0
- Gene
- icle0
- Outcome
- lipid utilizatione0
- Qualifier
- restrict the supply of carbon sources to the growing seedlinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“trehalose may inhibit lipid utilization by suppressing icl gene expression and enzyme activity and thus restrict the supply of carbon sources to the growing seedling”
t0“Arabidopsis”
The same questions in other papers
trehalose · molecular: expression · ArabidopsisResults agree
2 papers in the same direction as this paper
- no effect
- negative
infection · molecular: expression · ArabidopsisPositive and negative results
8 papers in the same direction as this paper · 19 in the opposite direction
- positive
- positive
- positive
24 papers more in this group
See all 28 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.