Abstract
Auxin is known to stimulate protein synthesis in many plant tissues, but the mechanisms involved in this process are unknown. The present research inquires whether auxin might regulate selective translation of mRNAs by inducing S6 ribosomal protein phosphorylation on the 40S ribosomal subunit in maize (Zea mays L.). Maize embryonic axes auxin-stimulated by natural (IAA) or synthetic (Dicamba or 1-NAA) auxins, selectively increased ribosomal protein synthesis. This effect was not reproduced by auxin inactive analogue 2-NAA. Enhanced S6 ribosomal protein phosphorylation on the 40S ribosomal subunit was also observed after auxin stimulation, as measured by [32P] incorporation into this protein. This increment did not occur when stimulation was performed with the inactive auxin analogue. Further, increased recruitment into polysomes of two 5'TOP-like mRNAs, encoding for the initiation translation factor eIF-iso4E and the S6 ribosomal protein, was also found after auxin stimulation of maize axes. A positive correlation was established between the levels of S6 ribosomal protein phosphorylation and the S6 ribosomal protein transcript recruitment into polysomes by means of okadaic acid or heat shock application to maize axes. These data indicate that auxin stimulates S6 ribosomal protein phosphorylation on maize ribosomes, concomitant to the recruitment of specific mRNAs (5'TOP-like mRNAs) into polysomes for translation. It is proposed that by this mechanism auxin regulate the synthesis of specific proteins in maize tissues.
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Claims 7
Claim · model-written summary
Auxin stimulation increased ribosomal protein synthesis in maize embryonic axes.
- Organ / stage
- embryonic axese0
- Treatment
- auxinse0
- Outcome
- ribosomal protein synthesise0
- Direction
- increasede0
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maize embryonic axes auxin-stimulated by natural (IAA) or synthetic (Dicamba or 1-NAA) auxins, selectively increased ribosomal protein synthesis.”
Claim · model-written summary
The inactive analogue 2-NAA did not reproduce the increase in ribosomal protein synthesis.
- Treatment
- auxin inactive analogue 2-NAAe0
- Outcome
- ribosomal protein synthesise1
- Direction
- not reproducede0
- Comparator
- auxinse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This effect was not reproduced by auxin inactive analogue 2-NAA.”
e1“Maize embryonic axes auxin-stimulated by natural (IAA) or synthetic (Dicamba or 1-NAA) auxins, selectively increased ribosomal protein synthesis.”
Claim · model-written summary
Auxin stimulation enhanced S6 ribosomal protein phosphorylation on the 40S subunit.
- Treatment
- auxin stimulatione0
- Gene
- S6 ribosomal proteine0
- Outcome
- S6 ribosomal protein phosphorylatione0
- Direction
- Enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Enhanced S6 ribosomal protein phosphorylation on the 40S ribosomal subunit was also observed after auxin stimulation”
e1“in maize (Zea mays L.)”
Claim · model-written summary
The inactive auxin analogue did not increase S6 phosphorylation.
- Treatment
- inactive auxin analoguee0
- Gene
- S6 ribosomal proteine1
- Outcome
- S6 ribosomal protein phosphorylatione1
- Direction
- did not occure0
- Comparator
- auxin stimulatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This increment did not occur when stimulation was performed with the inactive auxin analogue.”
e1“Enhanced S6 ribosomal protein phosphorylation on the 40S ribosomal subunit was also observed after auxin stimulation”
e2“in maize (Zea mays L.)”
Claim · model-written summary
Auxin stimulation increased polysome recruitment of eIF-iso4E and S6 protein mRNAs.
- Organ / stage
- axese0
- Treatment
- auxin stimulatione0
- Gene
- eIF-iso4E and the S6 ribosomal proteine0
- Outcome
- recruitment into polysomese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased recruitment into polysomes of two 5'TOP-like mRNAs, encoding for the initiation translation factor eIF-iso4E and the S6 ribosomal protein, was also found after auxin stimulation of maize axes”
Claim · model-written summary
S6 phosphorylation levels correlated positively with S6 transcript recruitment into polysomes under okadaic acid or heat shock.
- Treatment
- okadaic acid or heat shock applicatione0
- Gene
- S6 ribosomal proteine0
- Outcome
- S6 ribosomal protein transcript recruitment into polysomese0
- Direction
- positive correlatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A positive correlation was established between the levels of S6 ribosomal protein phosphorylation and the S6 ribosomal protein transcript recruitment into polysomes by means of okadaic acid or heat shock application to maize axes.”
Claim · model-written summary
The authors propose that S6 phosphorylation and transcript recruitment regulate synthesis of specific proteins in maize.
- Treatment
- auxine0
- Gene
- S6 ribosomal proteine1
- Outcome
- synthesis of specific proteinse0
- Qualifier
- recruitment of specific mRNAse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It is proposed that by this mechanism auxin regulate the synthesis of specific proteins in maize tissues.”
e1“These data indicate that auxin stimulates S6 ribosomal protein phosphorylation on maize ribosomes, concomitant to the recruitment of specific mRNAs (5'TOP-like mRNAs) into polysomes for translation.”
The same questions in other papers
class · quality: protein · Zea mays (maize)Positive and negative results
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- no effect
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See all 12 papers →class · molecular: expression · Zea mays (maize)Positive and negative results
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- negative
- no effect
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See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.