Abstract
Abstract Effective utilization of cell and tissue culture methods in Zea mays research requires cultures capable of plant regeneration. These differentiated plants would provide a direct link with conventional genetic and breeding procedures. Maize callus from embryo scutellar tissues was initiated and maintained on MS medium inorganic components, Straus medium vitamins and amino acids, 20 g sucrose and 8 g agar per liter, and 2 mg 2,4‐dichlorophenoxyacetic acid (2,4‐D)/liter. Callus has been maintained subculture every 21 to 28 days and has remained capable of differentiation for 9 months. Regeneration of complete plants was accomplished by subculture of callus to 0.25 mg 2,4‐D/liter for 30 days followed by transfer to 2,4‐D‐free culture medium. At 0.25 mg 2,4‐D/liter numerous curled and wrinkled leaves developed. Approximately 200 complete plants have been differentiated. after transfer to the 2,4‐D‐free medium. Root tip cells from five plants indicated that each had 20 chromosomes. After transplantation to soil, 10 to 15% of the plants survived and grew normally. The optimum embryo age for scutellar callus initiation was 18 days post‐pollination. Hormone combinations such as 1 mg 2,4‐D, 4 mg a‐naphthaleneacetic acid (NAA), and 0.05 mg 6‐(γ,γ‐dimethyl allylamino)‐purine (2iP)/liter may increase the efficiency of scutellar callus initiation.
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Claims 7
Claim · model-written summary
Maize callus remained capable of differentiation for 9 months.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maize callus from embryo scutellar tissues was initiated and maintained on MS medium”
e1“Callus has been maintained subculture every 21 to 28 days and has remained capable of differentiation for 9 months”
Claim · model-written summary
Complete maize plants were regenerated from callus after 2,4-D subculture and transfer to 2,4-D-free medium.
- Treatment
- subculture of callus to 0.25 mg 2,4‐D/liter for 30 days followed by transfer to 2,4‐D‐free culture mediume2
- Outcome
- Regeneration of complete plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maize callus from embryo scutellar tissues was initiated and maintained on MS medium”
e2“Regeneration of complete plants was accomplished by subculture of callus to 0.25 mg 2,4‐D/liter for 30 days followed by transfer to 2,4‐D‐free culture medium”
Claim · model-written summary
Approximately 200 complete maize plants were differentiated.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maize callus from embryo scutellar tissues was initiated and maintained on MS medium”
e3“Approximately 200 complete plants have been differentiated”
Claim · model-written summary
Root tip cells of five regenerated maize plants each had 20 chromosomes.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maize callus from embryo scutellar tissues was initiated and maintained on MS medium”
e4“Root tip cells from five plants indicated that each had 20 chromosomes”
Claim · model-written summary
After transplantation to soil, 10 to 15% of regenerated maize plants survived.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maize callus from embryo scutellar tissues was initiated and maintained on MS medium”
e5“After transplantation to soil, 10 to 15% of the plants survived and grew normally”
Claim · model-written summary
The optimum embryo age for scutellar callus initiation was 18 days post-pollination.
- Organ / stage
- embryoe6
- Outcome
- optimum embryo age for scutellar callus initiatione6
- Value
- 18 days post‐pollinatione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maize callus from embryo scutellar tissues was initiated and maintained on MS medium”
e6“The optimum embryo age for scutellar callus initiation was 18 days post‐pollination”
Claim · model-written summary
Hormone combinations of 2,4-D, NAA and 2iP may increase the efficiency of scutellar callus initiation.
- Treatment
- Hormone combinations such as 1 mg 2,4‐De7
- Outcome
- efficiency of scutellar callus initiatione7
- Direction
- increasee7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maize callus from embryo scutellar tissues was initiated and maintained on MS medium”
e7“Hormone combinations such as 1 mg 2,4‐D, 4 mg a‐naphthaleneacetic acid (NAA), and 0.05 mg 6‐(γ,γ‐dimethyl allylamino)‐purine (2iP)/liter may increase the efficiency of scutellar callus initiation”
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