Abstract
An efficient method for in vitro micro propagation and genetic transformation of plants are crucial for both basic and applied research. Maize is one of the most important cereal crops around the world. Regeneration from immature embryo is hampered due to its unavailability round the year. On the contrary mature embryo especially tropical maize is recalcitrant toward tissue culture. Here we report a highly efficient regeneration (90%) system for maize by using 2 different approaches i.e., embryogenic and organogenic callus cultures. Seeds were germinated on MS medium supplemented with 5 mg/l 2,4-D and 3 mg/l BAP. Nodal regions of 2 wks old seedlings were longitudinally split upon isolation and subsequently placed on callus initiation medium. The maximum frequency of embryogenic callus formation (90%) was obtained on MS medium supplemented with 2 mg/l 2,4-D and 1 mg/l BAP in the dark conditions. The compact granular organogenic callus formation (85% frequency) was obtained on MS medium supplemented with 2.5 mg/l 2,4-D and 1.5 mg/l BAP at light conditions. MS medium supplemented with 2 mg/l BAP, 1 mg/l Kinetin and 0.5 mg/l NAA promoted the highest frequency of shoot induction. The highest frequency of root formation was observed when shoots were grown on MS medium. The regenerated plants were successfully hardened in earthen pots after adequate acclimatization. The important advantage of this improved method is shortening of regeneration time by providing an efficient and rapid regeneration tool for obtaining more stable transformants from mature seeds of Indian tropical maize cultivar (HQPM-1).
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Claims 7
Claim · model-written summary
The maize regeneration system reached 90% efficiency using embryogenic and organogenic callus approaches.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here we report a highly efficient regeneration (90%) system for maize by using 2 different approaches i.e., embryogenic and organogenic callus cultures.”
Claim · model-written summary
MS medium with 2 mg/l 2,4-D and 1 mg/l BAP in darkness produced 90% embryogenic callus formation.
- Treatment
- MS medium supplemented with 2 mg/l 2,4-D and 1 mg/l BAPe0
- Outcome
- embryogenic callus formatione0
- Direction
- maximume0
- Value
- 90%e0
- Setting
- dark conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maximum frequency of embryogenic callus formation (90%) was obtained on MS medium supplemented with 2 mg/l 2,4-D and 1 mg/l BAP in the dark conditions.”
t0“maize”
Claim · model-written summary
MS medium with 2.5 mg/l 2,4-D and 1.5 mg/l BAP under light produced 85% organogenic callus formation.
- Treatment
- MS medium supplemented with 2.5 mg/l 2,4-D and 1.5 mg/l BAPe0
- Outcome
- organogenic callus formatione0
- Value
- 85% frequencye0
- Setting
- light conditionse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The compact granular organogenic callus formation (85% frequency) was obtained on MS medium supplemented with 2.5 mg/l 2,4-D and 1.5 mg/l BAP at light conditions.”
t0“maize”
Claim · model-written summary
MS medium with BAP, kinetin and NAA promoted the highest shoot induction frequency.
- Treatment
- MS medium supplemented with 2 mg/l BAP, 1 mg/l Kinetin and 0.5 mg/l NAAe0
- Outcome
- frequency of shoot inductione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS medium supplemented with 2 mg/l BAP, 1 mg/l Kinetin and 0.5 mg/l NAA promoted the highest frequency of shoot induction.”
t0“maize”
Claim · model-written summary
Shoot growth on MS medium yielded the highest root formation frequency.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest frequency of root formation was observed when shoots were grown on MS medium.”
t0“maize”
Claim · model-written summary
Regenerated plants were hardened successfully in earthen pots after acclimatization.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The regenerated plants were successfully hardened in earthen pots after adequate acclimatization.”
t0“maize”
Claim · model-written summary
The method shortened regeneration time for mature seeds of Indian tropical maize HQPM-1.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The important advantage of this improved method is shortening of regeneration time by providing an efficient and rapid regeneration tool for obtaining more stable transformants from mature seeds of Indian tropical maize cultivar (HQPM-1).”
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