Paper · 1990 · Pesticide Science

Imidazolinone inhibition of acetohydroxyacid synthase in vitro and in vivo

Mark A. Stidham; Dale L. Shaner

3
claims
6
verbatim quotes
18
citations
2
research areas
—
Not open access

Abstract

Abstract Imidazolinone herbicides inhibit the first enzyme of branched‐chain amino acid biosynthesis, acetohydroxyacid synthase. The inhibition of the enzyme in vitro by these herbicides increases with incubation time, but is not irreversible, as deduced by reaction progress curves. In contrast to this result is the apparent irreversible inhibition of the enzyme by these imidazolinones that occurs when the herbicide is applied to intact corn plants. Plants treated with imidazolinone herbicide and then extracted showed dramatically reduced enzyme activity. This effect on extractable enzyme level occurred with several different imidazolinone herbicides in either foliar or soil application. The decrease in extractable enzyme activity could be observed within four hours after treatment. Herbicides other than imidazolinones did not reduce the extractable enzyme level. These findings suggest that the enzyme‐ imidazolinone interaction in vivo may be different from the interaction observed in vitro.

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Claims 3

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Corn plants treated with imidazolinone herbicide and then extracted showed dramatically reduced enzyme activity.

Treatment
imidazolinone herbicidee1
Outcome
enzyme activitye1
Direction
reducede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“intact corn plants”

✓ located verbatim in the source · abstract, characters 434–452 · show in text ↑
e1

“Plants treated with imidazolinone herbicide and then extracted showed dramatically reduced enzyme activity”

✓ located verbatim in the source · abstract, characters 454–560 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The decrease in extractable enzyme activity in corn could be observed within four hours after treatment.

Outcome
decrease in extractable enzyme activitye1
Qualifier
within four hours after treatmente1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“intact corn plants”

✓ located verbatim in the source · abstract, characters 434–452 · show in text ↑
e1

“The decrease in extractable enzyme activity could be observed within four hours after treatment”

✓ located verbatim in the source · abstract, characters 697–792 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Herbicides other than imidazolinones did not reduce the extractable enzyme level in corn.

Treatment
Herbicides other than imidazolinonese1
Outcome
extractable enzyme levele1
Direction
did not reducee1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“intact corn plants”

✓ located verbatim in the source · abstract, characters 434–452 · show in text ↑
e1

“Herbicides other than imidazolinones did not reduce the extractable enzyme level”

✓ located verbatim in the source · abstract, characters 794–874 · show in text ↑

herbicide · physiology: enzyme activity · Zea mays (maize)Positive and negative results

4 papers: 1 positive, 3 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. no effect
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.