Paper · 1990 · Photochemistry and Photobiology

PHYTOCHROME INDUCTION OF PHOTOREACTIVATING ENZYME IN Phaseolus vulgaris L. SEEDLINGS*

Bernd Langer; Eckard Wellmann

6
claims
12
verbatim quotes
52
citations
2
research areas
—
Not open access

Abstract

Abstract— Photoreactivating enzyme (PRE) activity was measured in hypocotyls of Phaseolus vulgaris L. seedlings using a radioimmunoassay for thymine dimers. In dark‐grown seedlings a five‐fold increase in PRE activity was observed after 6 h of irradiation with blue or far‐red light. Short time irradiations with red light were also effective. Reversibility of this red‐light‐effect by a subsequent short term irradiation with far‐red light and also the high effectiveness of continuous far‐red light indicate that PRE activity is under phytochrome control. This observation points to PRE induction via gene activation.

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Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Blue light produced a five-fold increase in PRE activity in dark-grown seedlings after 6 h.

Taxon
Phaseolus vulgaris L.Phaseolus vulgarist0
Treatment
bluee0
Gene
PREe0
Outcome
PRE activitye0
Direction
increasee0
Value
five‐folde0
Qualifier
after 6 he0
Comparator
dark‐grown seedlingse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In dark‐grown seedlings a five‐fold increase in PRE activity was observed after 6 h of irradiation with blue or far‐red light”

✓ located verbatim in the source · abstract, characters 157–282 · show in text ↑
t0

“Phaseolus vulgaris L.”

✓ located verbatim in the source · title, characters 53–74 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Far-red light produced a five-fold increase in PRE activity in dark-grown seedlings after 6 h.

Taxon
Phaseolus vulgaris L.Phaseolus vulgarist0
Treatment
far‐red lighte0
Gene
PREe0
Outcome
PRE activitye0
Direction
increasee0
Value
five‐folde0
Qualifier
after 6 he0
Comparator
dark‐grown seedlingse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In dark‐grown seedlings a five‐fold increase in PRE activity was observed after 6 h of irradiation with blue or far‐red light”

✓ located verbatim in the source · abstract, characters 157–282 · show in text ↑
t0

“Phaseolus vulgaris L.”

✓ located verbatim in the source · title, characters 53–74 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Brief red-light irradiation also raised PRE activity.

Taxon
Phaseolus vulgaris L.Phaseolus vulgarist0
Treatment
red lighte0
Outcome
irradiations with red lighte0
Direction
effectivee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Short time irradiations with red light were also effective”

✓ located verbatim in the source · abstract, characters 284–342 · show in text ↑
t0

“Phaseolus vulgaris L.”

✓ located verbatim in the source · title, characters 53–74 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Subsequent far-red light reversed the red-light effect on PRE activity.

Taxon
Phaseolus vulgaris L.Phaseolus vulgarist0
Treatment
subsequent short term irradiation with far‐red lighte0
Outcome
Reversibilitye0
Direction
Reversibilitye0
Comparator
red‐light‐effecte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Reversibility of this red‐light‐effect by a subsequent short term irradiation with far‐red light”

✓ located verbatim in the source · abstract, characters 344–440 · show in text ↑
t0

“Phaseolus vulgaris L.”

✓ located verbatim in the source · title, characters 53–74 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The light-response pattern indicates phytochrome control of PRE activity.

Taxon
Phaseolus vulgaris L.Phaseolus vulgarist0
Gene
phytochromee0
Outcome
PRE activitye0
Qualifier
phytochrome controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the high effectiveness of continuous far‐red light indicate that PRE activity is under phytochrome control”

✓ located verbatim in the source · abstract, characters 450–556 · show in text ↑
t0

“Phaseolus vulgaris L.”

✓ located verbatim in the source · title, characters 53–74 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The authors infer PRE induction via gene activation.

Taxon
Phaseolus vulgaris L.Phaseolus vulgarist0
Gene
PREe0
Outcome
PRE inductione0
Qualifier
gene activatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“This observation points to PRE induction via gene activation”

✓ located verbatim in the source · abstract, characters 558–618 · show in text ↑
t0

“Phaseolus vulgaris L.”

✓ located verbatim in the source · title, characters 53–74 · show in text ↑

light · physiology: enzyme activity · Phaseolus vulgaris (common bean)Positive and negative results

7 papers: 5 positive, 2 negative or no effect

4 papers in the same direction as this paper · 1 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. no effect
  3. positive

3 papers more in this group

See all 7 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.