Abstract
Tropospheric ozone pollution is described as having major negative effects on plants, compromising plant survival. Carbon metabolism is especially affected. In the present work, the effects of chronic ozone exposure were evaluated at the proteomic level in developing leaves of young poplar plants exposed to 120 ppb of ozone for 35 days. Soluble proteins (excluding intrinsic membrane proteins) were extracted from leaves after 3, 14 and 35 days of ozone exposure, as well as 10 days after a recovery period. Proteins (pI 4 to 7) were analyzed by 2-D DIGE experiments, followed by MALDI-TOF-TOF identification. Additional observations were obtained on growth, lesion formation, and leaf pigments analysis. Although treated plants showed large necrotic spots and chlorosis in mature leaves, growth decreased only slightly and plant height was not affected. The number of abscised leaves was higher in treated plants, but new leaf formation was not affected. A decrease in chlorophylls and lutein contents was recorded. A large number of proteins involved in carbon metabolism were identified. In particular, proteins associated with the Calvin cycle and electron transport in the chloroplast were down-regulated. In contrast, proteins associated with glucose catabolism increased in response to ozone exposure. Other identified enzymes are associated with protein folding, nitrogen metabolism and oxidoreductase activity.
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Claims 9
Claim · model-written summary
Ozone treated poplars developed necrotic spots and chlorosis in mature leaves.
PopulusNot reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treated plants showed large necrotic spots and chlorosis in mature leaves”
t0“poplar leaves”
Claim · model-written summary
Ozone treated poplars had slightly decreased growth but unaffected height.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“growth decreased only slightly and plant height was not affected”
e1“plants exposed to 120 ppb of ozone for 35 days”
t0“poplar leaves”
Claim · model-written summary
Ozone treatment did not affect poplar height.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plant height was not affected”
e1“plants exposed to 120 ppb of ozone for 35 days”
t0“poplar leaves”
Claim · model-written summary
Ozone treatment increased abscised leaves.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The number of abscised leaves was higher in treated plants”
e1“plants exposed to 120 ppb of ozone for 35 days”
t0“poplar leaves”
Claim · model-written summary
Ozone treatment did not affect new leaf formation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“new leaf formation was not affected”
e1“plants exposed to 120 ppb of ozone for 35 days”
t0“poplar leaves”
Claim · model-written summary
Ozone reduced chlorophyll and lutein contents.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A decrease in chlorophylls and lutein contents was recorded.”
e1“plants exposed to 120 ppb of ozone for 35 days”
t0“poplar leaves”
Claim · model-written summary
Ozone downregulated Calvin cycle and chloroplast electron transport proteins.
- Treatment
- 120 ppb of ozone for 35 dayse1
- Outcome
- proteins associated with the Calvin cycle and electron transport in the chloroplaste0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proteins associated with the Calvin cycle and electron transport in the chloroplast were down-regulated”
e1“plants exposed to 120 ppb of ozone for 35 days”
t0“poplar leaves”
Claim · model-written summary
Ozone increased proteins associated with glucose catabolism.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proteins associated with glucose catabolism increased in response to ozone exposure”
t0“poplar leaves”
Claim · model-written summary
Identified proteins included enzymes linked to folding, nitrogen metabolism and oxidoreductase activity.
- Outcome
- enzymes are associated with protein folding, nitrogen metabolism and oxidoreductase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Other identified enzymes are associated with protein folding, nitrogen metabolism and oxidoreductase activity.”
t0“poplar leaves”
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