Abstract
A cDNA fragment corresponding to an elongation factor lα subunit (EF‐lα) messenger was amplified and used to isolate a corresponding full‐length copy from an apple ( Malus domestica [L.] Borkh) cv. McIntosh ‘Wijcik’ cDNA library. Genomic Southern blot analysis using this probe confirmed that the EF‐1α factor is encoded by a multigenic family in apple. Northern blot analysis of corresponding transcript levels in apple tree organs at various developmental stages revealed that very young leaves contain higher levels of EF‐1α mRNA than older leaves, although significant levels could be detected in all organs tested. In response to wounding, a transient decrease in EF‐1α mRNA levels was observed in apple plantlets, followed by a slight increase. A 24‐h incubation at low temperature (5°C) induced a significant decrease in corresponding transcript abundance. In contrast, when dark‐grown, etiolated, apple plantlets were transferred to light conditions and analyzed with the same probe, few modifications in EF‐1α transcript abundance were detected.
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Claims 7
Claim · model-written summary
The EF-1alpha factor is encoded by a multigenic family in apple.
- Taxon
- apple
Malus domesticae1 - Gene
- EF‐1αe1
- Outcome
- encoded by a multigenic familye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Genomic Southern blot analysis using this probe confirmed that the EF‐1α factor is encoded by a multigenic family in apple”
Claim · model-written summary
Very young apple leaves contain higher EF-1alpha mRNA levels than older leaves.
- Taxon
- apple treeMalus domesticae2
- Organ / stage
- very young leavese2
- Gene
- EF‐1αe2
- Outcome
- levels of EF‐1α mRNAe2
- Direction
- highere2
- Comparator
- older leavese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Northern blot analysis of corresponding transcript levels in apple tree organs at various developmental stages revealed that very young leaves contain higher levels of EF‐1α mRNA than older leaves, although significant levels could be detected in all organs tested”
Claim · model-written summary
Significant EF-1alpha mRNA levels could be detected in all apple organs tested.
- Taxon
- apple treeMalus domesticae2
- Organ / stage
- all organs testede2
- Outcome
- significant levels could be detectede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Northern blot analysis of corresponding transcript levels in apple tree organs at various developmental stages revealed that very young leaves contain higher levels of EF‐1α mRNA than older leaves, although significant levels could be detected in all organs tested”
Claim · model-written summary
Wounding caused a transient decrease in EF-1alpha mRNA levels in apple plantlets.
- Taxon
- apple plantletsMalus domesticae3
- Treatment
- woundinge3
- Outcome
- EF‐1α mRNA levelse3
- Direction
- transient decreasee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“In response to wounding, a transient decrease in EF‐1α mRNA levels was observed in apple plantlets, followed by a slight increase”
Claim · model-written summary
After the transient decrease, EF-1alpha mRNA levels in apple plantlets showed a slight increase following wounding.
- Taxon
- apple plantletsMalus domesticae3
- Treatment
- woundinge3
- Outcome
- EF‐1α mRNA levelse3
- Direction
- slight increasee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“In response to wounding, a transient decrease in EF‐1α mRNA levels was observed in apple plantlets, followed by a slight increase”
Claim · model-written summary
A 24-h incubation at 5°C significantly decreased EF-1alpha transcript abundance in apple plants.
- Taxon
- apple plantsMalus domesticat0
- Treatment
- 24‐h incubation at low temperature (5°C)e4
- Outcome
- transcript abundancee4
- Direction
- decreasee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“apple plants”
e4“A 24‐h incubation at low temperature (5°C) induced a significant decrease in corresponding transcript abundance”
Claim · model-written summary
Transfer of dark-grown apple plantlets to light caused few modifications in EF-1alpha transcript abundance.
- Taxon
- apple plantletsMalus domesticae5
- Treatment
- transferred to light conditionse5
- Outcome
- EF‐1α transcript abundancee5
- Direction
- no_changeinferrede5
- Comparator
- dark‐grown, etiolatede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“when dark‐grown, etiolated, apple plantlets were transferred to light conditions and analyzed with the same probe, few modifications in EF‐1α transcript abundance were detected”
The same questions in other papers
light · molecular: expression · Malus domestica (apple)Positive and negative results
1 paper in the same direction as this paper · 6 reporting no difference from an alternative
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.