Paper · 1991 · PLANT PHYSIOLOGY

Light Microscopy Study of Nodule Initiation in Pisum sativum L. cv Sparkle and in Its Low-Nodulating Mutant E2 (sym 5)

Frédérique C. Guinel; Thomas A. LaRue

10
claims
20
verbatim quotes
50
citations
2
research areas
✓
Open access

Abstract

We compared nodule initiation in lateral roots of Pisum sativum (L.) cv Sparkle and in a low-nodulating mutant E2 (sym 5). In Sparkle, about 25% of the infections terminated in the epidermis, a similar number stopped in the cortex, and 50% resulted in the formation of a nodule meristem or an emerged nodule. The mutant E2 (sym 5) was infected as often as was the parent, and it formed a normal infection thread. In the mutant, cell divisions rarely occurred in advance of the infection thread, and few nodule primordia were produced. Growing the mutant at a low root temperature or adding Ag(+) to the substrate increased the number of cell divisions and nodule primordia. We conclude that, in the E2 line, the infection process is arrested in the cortex, at the stage of initial cell divisions before the establishment of a nodule primordium.

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Claims 10

Observationobservedoriginal resultmeasured

Claim · model-written summary

In Sparkle, about 25% of infections ended in the epidermis.

Taxon
Pisum sativumLathyrus oleraceust0
Material
Sparklee0
Outcome
infections terminated in the epidermise0
Value
about 25%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“In Sparkle, about 25% of the infections terminated in the epidermis”

✓ located verbatim in the source · abstract, characters 123–190 · show in text ↑
t0

“Pisum sativum L. cv Sparkle”

✓ located verbatim in the source · title, characters 47–74 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

In Sparkle, a similar number of infections stopped in the cortex.

Taxon
Pisum sativumLathyrus oleraceust0
Material
Sparklet0
Outcome
stopped in the cortexe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“a similar number stopped in the cortex”

✓ located verbatim in the source · abstract, characters 192–230 · show in text ↑
t0

“Pisum sativum L. cv Sparkle”

✓ located verbatim in the source · title, characters 47–74 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

In Sparkle, 50% of infections formed a meristem or emerged nodule.

Taxon
Pisum sativumLathyrus oleraceust0
Material
Sparklet0
Outcome
formation of a nodule meristem or an emerged nodulee0
Value
50%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“50% resulted in the formation of a nodule meristem or an emerged nodule”

✓ located verbatim in the source · abstract, characters 236–307 · show in text ↑
t0

“Pisum sativum L. cv Sparkle”

✓ located verbatim in the source · title, characters 47–74 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

E2 was infected as often as the parent.

Taxon
Pisum sativumLathyrus oleraceust0
Material
E2 (sym 5)e0
Outcome
infected as often as was the parente0
Comparator
parente0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“The mutant E2 (sym 5) was infected as often as was the parent”

✓ located verbatim in the source · abstract, characters 309–370 · show in text ↑
t0

“Pisum sativum L. cv Sparkle”

✓ located verbatim in the source · title, characters 47–74 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

E2 formed normal infection threads.

Taxon
Pisum sativumLathyrus oleraceust0
Material
E2 (sym 5)t0
Outcome
normal infection threade0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“it formed a normal infection thread”

✓ located verbatim in the source · abstract, characters 376–411 · show in text ↑
t0

“Pisum sativum L. cv Sparkle and in Its Low-Nodulating Mutant E2 (sym 5)”

✓ located verbatim in the source · title, characters 47–118 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

E2 rarely divided cells ahead of infection threads.

Taxon
Pisum sativumLathyrus oleraceust0
Material
E2 (sym 5)t0
Outcome
cell divisions rarely occurred in advance of the infection threade0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“In the mutant, cell divisions rarely occurred in advance of the infection thread”

✓ located verbatim in the source · abstract, characters 413–493 · show in text ↑
t0

“Pisum sativum L. cv Sparkle and in Its Low-Nodulating Mutant E2 (sym 5)”

✓ located verbatim in the source · title, characters 47–118 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

E2 produced few nodule primordia.

Taxon
Pisum sativumLathyrus oleraceust0
Material
E2 (sym 5)t0
Outcome
few nodule primordia were producede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“few nodule primordia were produced”

✓ located verbatim in the source · abstract, characters 499–533 · show in text ↑
t0

“Pisum sativum L. cv Sparkle and in Its Low-Nodulating Mutant E2 (sym 5)”

✓ located verbatim in the source · title, characters 47–118 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Low root temperature increased cell divisions and nodule primordia in E2.

Taxon
Pisum sativumLathyrus oleraceust0
Material
E2 (sym 5)t0
Treatment
a low root temperaturee0
Outcome
number of cell divisions and nodule primordiae0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Growing the mutant at a low root temperature or adding Ag(+) to the substrate increased the number of cell divisions and nodule primordia.”

✓ located verbatim in the source · abstract, characters 535–673 · show in text ↑
t0

“Pisum sativum L. cv Sparkle and in Its Low-Nodulating Mutant E2 (sym 5)”

✓ located verbatim in the source · title, characters 47–118 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Silver addition increased cell divisions and nodule primordia in E2.

Taxon
Pisum sativumLathyrus oleraceust0
Material
E2 (sym 5)t0
Treatment
adding Ag(+) to the substratee0
Outcome
number of cell divisions and nodule primordiae0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“adding Ag(+) to the substrate increased the number of cell divisions and nodule primordia”

✓ located verbatim in the source · abstract, characters 583–672 · show in text ↑
t0

“Pisum sativum L. cv Sparkle and in Its Low-Nodulating Mutant E2 (sym 5)”

✓ located verbatim in the source · title, characters 47–118 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Authors conclude E2 infection arrests in cortex before nodule primordium formation.

Taxon
Pisum sativumLathyrus oleraceust0
Material
E2e0
Outcome
infection process is arrested in the cortexe0
Qualifier
at the stage of initial cell divisions before the establishment of a nodule primordiume0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

e0

“in the E2 line, the infection process is arrested in the cortex, at the stage of initial cell divisions before the establishment of a nodule primordium.”

✓ located verbatim in the source · abstract, characters 692–844 · show in text ↑
t0

“Pisum sativum L. cv Sparkle”

✓ located verbatim in the source · title, characters 47–74 · show in text ↑

silver atom · symbiosis · Lathyrus oleraceus (pea)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

growing medium · symbiosis · Lathyrus oleraceus (pea)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.