Abstract
Abiotic stress limits seed germination, plant growth, flowering and fruit quality, causing economic decrease. Small Heat Shock Proteins (sHSPs) are chaperons with roles in stress tolerance. Herein, we report the functional characterization of a cytosolic class CI sHSP (OpsHSP18) from Opuntia streptacantha during seed germination in Arabidopsis thaliana transgenic lines subjected to different stress and hormone treatments. The over-expression of the OpsHSP18 gene in A. thaliana increased the seed germination rate under salt (NaCl) and osmotic (glucose and mannitol) stress, and in ABA treatments, compared with WT. On the other hand, the over-expression of the OpsHSP18 gene enhanced tolerance to salt (150 mM NaCl) and osmotic (274 mM mannitol) stress in Arabidopsis seedlings treated during 14 and 21 days, respectively. These plants showed increased survival rates (52.00 and 73.33%, respectively) with respect to the WT (18.75 and 53.75%, respectively). Thus, our results show that OpsHSP18 gene might have an important role in abiotic stress tolerance, in particular in seed germination and survival rate of Arabidopsis plants under unfavorable conditions.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
OpsHSP18 overexpression increased germination under NaCl stress versus WT.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Treatment
- over-expression of the OpsHSP18 genee0
- Gene
- OpsHSP18e0
- Outcome
- seed germination ratee0
- Direction
- increasede0
- Qualifier
- salt (NaCl)e0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The over-expression of the OpsHSP18 gene in A. thaliana increased the seed germination rate under salt (NaCl) and osmotic (glucose and mannitol) stress, and in ABA treatments, compared with WT”
t0“Arabidopsis thaliana”
Claim · model-written summary
OpsHSP18 overexpression increased germination under glucose osmotic stress versus WT.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- over-expression of the OpsHSP18 genee0
- Gene
- OpsHSP18e0
- Outcome
- seed germination ratee0
- Direction
- increasede0
- Qualifier
- glucosee0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The over-expression of the OpsHSP18 gene in A. thaliana increased the seed germination rate under salt (NaCl) and osmotic (glucose and mannitol) stress, and in ABA treatments, compared with WT”
t0“Arabidopsis thaliana”
Claim · model-written summary
OpsHSP18 overexpression increased germination under mannitol osmotic stress versus WT.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- over-expression of the OpsHSP18 genee0
- Gene
- OpsHSP18e0
- Outcome
- seed germination ratee0
- Direction
- increasede0
- Qualifier
- mannitole0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The over-expression of the OpsHSP18 gene in A. thaliana increased the seed germination rate under salt (NaCl) and osmotic (glucose and mannitol) stress, and in ABA treatments, compared with WT”
t0“Arabidopsis thaliana”
Claim · model-written summary
OpsHSP18 overexpression increased germination under ABA treatment versus WT.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- over-expression of the OpsHSP18 genee0
- Gene
- OpsHSP18e0
- Outcome
- seed germination ratee0
- Direction
- increasede0
- Qualifier
- ABA treatmentse0
- Comparator
- WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The over-expression of the OpsHSP18 gene in A. thaliana increased the seed germination rate under salt (NaCl) and osmotic (glucose and mannitol) stress, and in ABA treatments, compared with WT”
t0“Arabidopsis thaliana”
Claim · model-written summary
OpsHSP18 seedlings had 52.00% survival under 150 mM NaCl versus WT at 18.75%.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- survival ratese0
- Direction
- increasede0
- Value
- 52.00e0
- Qualifier
- 18.75e0
- Comparator
- WTe0
Not reportedTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These plants showed increased survival rates (52.00 and 73.33%, respectively) with respect to the WT (18.75 and 53.75%, respectively)”
t0“Arabidopsis thaliana”
Claim · model-written summary
OpsHSP18 seedlings had 73.33% survival under 274 mM mannitol versus WT at 53.75%.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- survival ratese0
- Direction
- increasede0
- Value
- 73.33%e0
- Qualifier
- 53.75%e0
- Comparator
- WTe0
Not reportedTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These plants showed increased survival rates (52.00 and 73.33%, respectively) with respect to the WT (18.75 and 53.75%, respectively)”
t0“Arabidopsis thaliana”
Claim · model-written summary
OpsHSP18 may play a role in abiotic stress tolerance in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- OpsHSP18e0
- Outcome
- abiotic stress tolerancee0
- Qualifier
- seed germination and survival ratee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OpsHSP18 gene might have an important role in abiotic stress tolerance, in particular in seed germination and survival rate of Arabidopsis plants under unfavorable conditions”