Abstract
Membrane deterioration differs in aging and senescent tissues. Involvement of free radicals in the process is generally recognized. Little is known about the physiological effects of gamma irradiation on plant tissues. Degradation of microsomal membranes by the action of free radicals, generated in vivo by gamma rays, was investigated. Cauliflower florets (Brassica oleracea L., Botrytis group) were exposed to 2 or 4 kiloGray of gamma radiation. Membrane deterioration was assessed during 8-day storage at 13 degrees C. Some senescence was indicated in nonirradiated controls by a parallel depletion of lipid phosphate and protein. Irradiation caused an immediate increase in tissue electrolyte leakage and a small increase in the free fatty acid content of membranes. In irradiated samples, leakage of electrolytes and the ratios of sterol to phospholipid and of free fatty acid to phospholipid increased with storage. During this period, membrane protein was progressively lost and the lipid phosphate-to-protein ratio increased markedly. Polyunsaturated fatty acids were selectively depleted from the free fatty acid fraction for all treatments, suggesting lipoxygenase activity. No change in lipid saturation was observed in the polar lipid fraction. The results suggest an enzyme-catalyzed senescence-like membrane deterioration, probably induced by chemical deesterification of phospholipids by free radicals generated during irradiation.
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Claims 11
Claim · model-written summary
Nonirradiated cauliflower florets showed parallel lipid phosphate and protein depletion.
- Taxon
- Cauliflower
Brassica oleracea var. botrytist0 - Treatment
- nonirradiated controlse0
- Outcome
- parallel depletion of lipid phosphate and proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Some senescence was indicated in nonirradiated controls by a parallel depletion of lipid phosphate and protein.”
t0“Cauliflower”
Claim · model-written summary
Irradiation immediately increased tissue electrolyte leakage.
- Taxon
- CauliflowerBrassica oleracea var. botrytist0
- Treatment
- Irradiatione0
- Outcome
- tissue electrolyte leakagee0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Irradiation caused an immediate increase in tissue electrolyte leakage and a small increase in the free fatty acid content of membranes.”
t0“Cauliflower”
Claim · model-written summary
Irradiation caused a small immediate increase in membrane free fatty acid content.
- Taxon
- CauliflowerBrassica oleracea var. botrytist0
- Treatment
- Irradiatione0
- Outcome
- free fatty acid content of membranese0
- Direction
- increasee0
- Qualifier
- smalle0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Irradiation caused an immediate increase in tissue electrolyte leakage and a small increase in the free fatty acid content of membranes.”
t0“Cauliflower”
Claim · model-written summary
Electrolyte leakage in irradiated cauliflower increased during storage.
- Taxon
- CauliflowerBrassica oleracea var. botrytist0
- Treatment
- irradiated samplese0
- Outcome
- leakage of electrolytese0
- Direction
- increasede0
- Qualifier
- with storagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In irradiated samples, leakage of electrolytes and the ratios of sterol to phospholipid and of free fatty acid to phospholipid increased with storage.”
t0“Cauliflower”
Claim · model-written summary
Sterol to phospholipid ratio increased during storage after irradiation.
- Taxon
- CauliflowerBrassica oleracea var. botrytist0
- Treatment
- irradiated samplese0
- Outcome
- ratios of sterol to phospholipide0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In irradiated samples, leakage of electrolytes and the ratios of sterol to phospholipid and of free fatty acid to phospholipid increased with storage.”
t0“Cauliflower”
Claim · model-written summary
Free fatty acid to phospholipid ratio increased during storage after irradiation.
- Taxon
- CauliflowerBrassica oleracea var. botrytist0
- Treatment
- irradiated samplese0
- Outcome
- free fatty acid to phospholipide0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In irradiated samples, leakage of electrolytes and the ratios of sterol to phospholipid and of free fatty acid to phospholipid increased with storage.”
t0“Cauliflower”
Claim · model-written summary
Membrane protein was progressively lost in irradiated cauliflower during storage.
- Taxon
- CauliflowerBrassica oleracea var. botrytist0
- Outcome
- membrane proteine0
- Direction
- progressively loste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During this period, membrane protein was progressively lost and the lipid phosphate-to-protein ratio increased markedly.”
t0“Cauliflower”
Claim · model-written summary
Lipid phosphate to protein ratio increased markedly during storage.
- Taxon
- CauliflowerBrassica oleracea var. botrytist0
- Outcome
- lipid phosphate-to-protein ratioe0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During this period, membrane protein was progressively lost and the lipid phosphate-to-protein ratio increased markedly.”
t0“Cauliflower”
Claim · model-written summary
Polyunsaturated fatty acids were selectively depleted from the free fatty acid fraction under all treatments.
- Taxon
- CauliflowerBrassica oleracea var. botrytist0
- Outcome
- Polyunsaturated fatty acidse0
- Direction
- selectively depletede0
- Qualifier
- for all treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Polyunsaturated fatty acids were selectively depleted from the free fatty acid fraction for all treatments, suggesting lipoxygenase activity.”
t0“Cauliflower”
Claim · model-written summary
Polar lipid saturation did not change.
- Taxon
- CauliflowerBrassica oleracea var. botrytist0
- Organ / stage
- polar lipid fractione0
- Outcome
- lipid saturatione0
- Direction
- No changee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No change in lipid saturation was observed in the polar lipid fraction.”
t0“Cauliflower”
Claim · model-written summary
Authors infer enzyme-catalyzed senescence-like membrane deterioration induced by deesterification from irradiation-generated radicals.
- Taxon
- CauliflowerBrassica oleracea var. botrytist0
- Outcome
- senescence-like membrane deterioratione0
- Qualifier
- chemical deesterification of phospholipids by free radicalse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results suggest an enzyme-catalyzed senescence-like membrane deterioration, probably induced by chemical deesterification of phospholipids by free radicals generated during irradiation.”
t0“Cauliflower”
The same questions in other papers
radiation · physiology: oxidative · Brassica oleracea (wild cabbage)Positive and negative results
2 in the opposite direction
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.